Outdoor HEPA filter Collection in Sante Fe, NM

[Guest guest]

http://www.carnicom.com/bio10.htm

ERYTHROCYTES, MATRIX & MOTILE BACTERIA

Clifford E Carnicom

Dec 17 2002

Microscopic sessions have again been conducted upon particulates collected

from an outdoor HEPA filter in operation at Santa Fe, NM. It is with some

dismay that I must report that the earlier findings of unexpected biological

components have again been repeated. There now exists a continuous record

of similar findings over a two to three year period, and the issue has now

evolved far beyond that of debate alone. The best analysis available from a

combination of sources continues to affirm the identification of the shown

biological components as being that of erythrocytes, or red blood cells.

The refusal of the United States Environmental Protection Agency to identify

certain fibrous materials and biological components within that material is

deeply entrenched into these current findings.

Any efforts to confirm, refute, discredit or affirm the current findings

must be done in a public venue under professional laboratory conditions with

independent verification. It is acknowledged that the laboratory facilities

available to this researcher are extremely limited in scope, and they are

inadequate to allow final judgment on the grave concerns that are raised.

The repetition of the findings from multiple methods and sources across a

long span of time is sufficient to mandate that such public and professional

testing now occur. The interests of the public welfare must be served in a

legitimate manner, and inflammatory debate prior to those test results is a

moot exercise. In the interim, citizens may wish to consider and act upon

the health implications inherent within this report.

(photo)

Biological components extracted from HEPA filter

Week of Dec 8 - 15 2002, Santa Fe NM

Approx. Magnification 5000x

Cell size approx 6-7 microns

'Matrix " material visible on left side of microphotograph

Concavities characteristic of erythrocytes visible under close inspection.

The samples identified appear to be composed of three primary structural

features, which are visible using multiple stain techniques:

The first component is that of the apparent erythrocytes. These usually

occur in clusters, although they occasionally occur individually. The cells

appear to be of a desiccated form, as has been mentioned and inferred from

the onset of these findings over three years ago. This conclusion is based

upon the measurement of the cells in their original form, which approximates

4 microns. A table of blood cell sizes for various species is available at

this page: Biological Operations Confirmed (Feb 25 2001). When the cells

are subjected to certain solutes, however, the cells enlarge and attain a

final size of approximately 6 to 7 microns; this size approximates that of

the human cell. Eosin and iodine stains have been used in the current

investigation; iodine appears to play a role in the reconstitution to the

larger size. Upon extended exposure to the iodine stain, the cells will

eventually become distorted and the characteristic biconcavities will

diminish. The stain used, eosin or iodine, is of value primarily for the

visual detection of the matrix structure, and to enhance contrast for the

cell structures shown.

The second component within this discussion is to be designated as the

'matrix' material. The composition of this structure is unidentified in all

ways, other than it appears to be of a definite biological nature. The term

'matrix' has been contributed by an independent researcher involved in the

video microscopy session referred to earlier, where a binding structure

within the earlier fibrous sample was observed and noted by that individual.

The term is used in the same sense here, as it appears this granular

material may also serve a binding function. It may also serve as a nutrient

source to the bacteria which will be described later. The cells are usually

found to be interspersed within this matrix material. The matrix is

distinctively visible under both eosin and iodine stains.

The third and final component has not been observed in prior sessions, and

is a result of modifications to the sampling method that have developed.

The method of sampling will be described in more detail at a later stage.

In the presence of a dilute eosin stain, a motile form of bacteria can be

repeatedly observed circulating in and about the granular matrix structure.

The bacteria (presumed) are extremely small in size, and are estimated at

1-2 microns in size. This size range is at the limit of visible light

microscopy and thus further evaluation will be difficult from this station.

The bacteria appear frequently in a combined or colony form, and they appear

to be attracted to the matrix - cellular structures. This observed behavior

is the basis of suggestion for the matrix serving as a potential nutrient

source for the bacteria. The dilute eosin stain makes the bacteria visible

and does not cause immediate death; the use of iodine stain in contrast

immediately kills these bacteria under observation. The species of bacteria

observed requires prompt identification, along with all other components

that are shown. The ability to observe these bacteria in a live form is a

direct result of the solution method of sampling that has been incorporated

into the current research.

Biological components extracted from HEPA filter

Week of Dec 8 - 15 2002, Santa Fe NM

Approx. Magnification 5000x

Cell size approx 6-7 micron

'Matrix " material visible on left side of microphotographs

Concavities characteristic of erythrocytes visible under close inspection.

It may be beneficial to the reader to be familiar with the history of

research on this topic, which now demonstrates a remarkable consistency in

the results that have been observed. Earlier research over the last two to

three years, at a minimum, is available on the following pages:

Biological Components Identified, May 11 2000

Additional Biological Components Identified, July 21 2000

Biological Operations Confirmed, Feb 25 2001

HEPA Biologicals Confirmed Mar 06 2001

Colorado HEPA Biologicals Confirmed Mar 16 2001

Biologicals Reaffirmed, April 08 2001

Identification Requested, April 19 2001

Erythrocytes : Positive Visual Identification May 03 2001

Erythrocytes : May 22 2001

EPA Refuses to Identify, Returns Sample, 18 Month Delay Jul 25 2001

Biological components extracted from HEPA filter

Week of Dec 8 - 15 2002, Santa Fe NM

Approx. Magnification 500x

Cell size approx 6-7 micron

'Matrix " material visible across span of sample.

Concavities characteristic of erythrocytes visible with shading.

The presentation of the microphotographs on this page at a magnification of

approximately 5000 times is an extension to typical light microscopy. The

normal limit of magnification with visible light microscopy is on the order

of 1000x to 2000x, and the collection of adequate light at the higher

magnification levels becomes increasingly difficult. A method has been

developed which combines the use of a digital eyepiece(CCD-charge coupled

device) with a conventional microscope objective. This method, combined

with the use of oil immersion techniques, has provided for the greater

magnification of images shown here. Availability of adequate light as well

as resolution remain as limiting factors as to what can be accomplished with

available equipment..

PRELIMINARY VIDEO OF INDIVIDUAL BACTERIA IN MOTION

OUTSIDE BOUNDARY OF MATRIX MATERIAL

(.AVI FILE , 10sec., 370K, Download and Use Windows Media Player)

Peculiar double nuclei structure.

Isolated example found and photographed.

Magnification approx 500x.

Note similarity to image captured from fibrous material video

on May 11, 2000

(Dark Field Microscopy Session)

May 11 2000 Biological Component Report

The method of collection and sampling is as follows:

A HEPA filter has again been placed outdoors in a highly rural environment.

The elevation of the filter during the most recent session is at

approximately 6 feet above ground level. Earlier sessions have had the

filter approximately 12 feet above ground level. The filter was operated

full time for approximately 5 days before the sampling process began. The

filter element was temporarily removed, and small sections of the filter

were removed with small scissors before returning the filter to operation

outdoors. Approximately 3 to 4 sections of the paper filter element

approximately 5mm by 15mm were removed for each trial. The small cuts from

the filter element were then placed into approximately 3-4ml of distilled

water within a test tube and allowed to stand for approximately 15 minutes

at room temperature. Some trials allowed the solution to be warmed to

approximately 75-80deg F. with external heat, and also the test tube was

shaken at various intervals. Two drops of the solution were then placed

onto a microscope slide. Initial tests were conducted with the use of eosin

stain, which allows cytoplasm detection. One drop of eosin stain was added

to the two drops of solution on the slide and a slide cover placed on top to

create a wet slide. Detection and magnification of the various components

up to a maximum of 5000x was then accomplished. Additional trials used

iodine stains, with the differences in visibility of the various components

as described above. The use of the dilute eosin stain allows the bacteria

to be observed alive; the use of the iodine stain appears favorable to the

matrix detection. Sampling was conducted consecutively for approximately 5

days with the same results being achieved on each occasion.