Guest guest Posted December 8, 2008 Report Share Posted December 8, 2008 The term " electroporation " refers to the use of a very powerful jolt of electricity applied to a cell for a very brief period of time, only a fraction of a second. It opens the cell's " pores " temporarily. They usually close up again after 5 seconds or less. With me so far? No? Ok, think of something stuck in your throat. Someone slaps you on the back. Your mouth opens very wide, you blast out the food. Same idea here but in reverse. The cell is hit with electricity. It opens its mouth suddenly. And there, right next to its mouth is some experimental DNA put there by scientists. The cell swallows it, and closes its mouth. The process of opening the pores up is called " electroPORATION " . The process of the cell swallowing the DNA is called " transfection " from " trans=across " " fect=make happen " . Knew that Latin class would come in handy someday. But this is done with voltages in excess of 1000 volts, not 3 or 6. It only happens when it is first applied, too. Once it is " on " the pores close up anyway. They don't stay open if the electricity stays on. It is only the spike of the first shock that opens the pores. Beck's device caused spikes. He thought that could open cell pores. Well it probably does but to so small an extent at the low voltage that it would not allow anything, not even a molecule, to enter the cell. Some people reported " interractions " however, and Beck was concerned about that. So he figured there might be a transfection effect. If there is one, however, it would not be an " electroporation " effect, but possibly some other way the electricity could cause drug interractions. Here's some possible ways besides transfection: 1. Electricity of low power tends to raise cell metabolic rates. The cells could simply get more active and consume more of whatever is around, drugs, vitamins, herbs, caffeine, alcohol, etc. 2. Electricity could cause some subtle effects on the drugs or chemicals themselves, making them act differently in the body. 3. Electricity could act as a catalyst and allow things to combine that normally would not. Transfection does not significantly occur with DC current. It is highest when current is pulsed on and off, or reverses direction about once a second at high enough voltages to open up cells walls. We don't come anywhere near the voltages needed. If you could elecroporate cells with a common battery, the companies that make the multi-thousand dollar electroporators would be out of business. It takes a pretty powerful, ruggedly built machine to deliver the effect, and we are not likely to be electroporating cells. One or two people have reported drug/chemical interractions. It's always better to electrify first, then take meds, etc, rather than the other way around, in case there is any effect. In the vast majority of cases nothing bad was reported. SOTA instruments sells more Beck devices than anyone and in all their years they have not had any reports of the effects Beck mentioned from interractions with substances in the body and electricity applied to the bloodstream. This comes up a lot, but we don't get significant repeatable reports of issues. What we do see is sore fingers from electrifying the bloodstream on wrist arteries and not reversing the current every 5-10 minutes. Someone might get sore fingers, but it has always gone away. I'd like to hear of any trouble like this so we can avoid it. bG Quote Link to comment Share on other sites More sharing options...
Guest guest Posted December 10, 2008 Report Share Posted December 10, 2008 O.K. this makes sense, thanks! How do you reverse the current ? I guess by switching the electrodes ? Does this apply to the Godzilla settings as well? Then,my assumption is wrong that the 2sec, 1.5min, 3min settings mean that the current gets reversed at these time intervals? > > The term " electroporation " refers to the use of a very powerful jolt > of electricity applied to a cell for a very brief period of time, > only a fraction of a second. It opens the cell's " pores " > temporarily. They usually close up again after 5 seconds or less. > With me so far? > > No? Ok, think of something stuck in your throat. Someone slaps you > on the back. Your mouth opens very wide, you blast out the food. > Same idea here but in reverse. The cell is hit with electricity. It > opens its mouth suddenly. And there, right next to its mouth is some > experimental DNA put there by scientists. The cell swallows it, and > closes its mouth. The process of opening the pores up is > called " electroPORATION " . The process of the cell swallowing the DNA > is called " transfection " from " trans=across " " fect=make happen " . > Knew that Latin class would come in handy someday. > > But this is done with voltages in excess of 1000 volts, not 3 or 6. > It only happens when it is first applied, too. Once it is " on " the > pores close up anyway. They don't stay open if the electricity stays > on. It is only the spike of the first shock that opens the pores. > > Beck's device caused spikes. He thought that could open cell pores. > Well it probably does but to so small an extent at the low voltage > that it would not allow anything, not even a molecule, to enter the > cell. > > Some people reported " interractions " however, and Beck was concerned > about that. So he figured there might be a transfection effect. If > there is one, however, it would not be an " electroporation " effect, > but possibly some other way the electricity could cause drug > interractions. > > Here's some possible ways besides transfection: > 1. Electricity of low power tends to raise cell metabolic rates. The > cells could simply get more active and consume more of whatever is > around, drugs, vitamins, herbs, caffeine, alcohol, etc. > > 2. Electricity could cause some subtle effects on the drugs or > chemicals themselves, making them act differently in the body. > > 3. Electricity could act as a catalyst and allow things to combine > that normally would not. > > Transfection does not significantly occur with DC current. It is > highest when current is pulsed on and off, or reverses direction > about once a second at high enough voltages to open up cells walls. > We don't come anywhere near the voltages needed. > > If you could elecroporate cells with a common battery, the companies > that make the multi-thousand dollar electroporators would be out of > business. It takes a pretty powerful, ruggedly built machine to > deliver the effect, and we are not likely to be electroporating > cells. > > One or two people have reported drug/chemical interractions. It's > always better to electrify first, then take meds, etc, rather than > the other way around, in case there is any effect. > > In the vast majority of cases nothing bad was reported. SOTA > instruments sells more Beck devices than anyone and in all their > years they have not had any reports of the effects Beck mentioned > from interractions with substances in the body and electricity > applied to the bloodstream. > > This comes up a lot, but we don't get significant repeatable reports > of issues. > > What we do see is sore fingers from electrifying the bloodstream on > wrist arteries and not reversing the current every 5-10 minutes. > Someone might get sore fingers, but it has always gone away. I'd > like to hear of any trouble like this so we can avoid it. > > bG > Quote Link to comment Share on other sites More sharing options...
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