Some research on the three mushrooms

[Guest guest]

Sally,

Reishi:

Ganoderic acid produced from submerged culture of Ganoderma lucidum

induces cell cycle arrest and cytotoxicity in human hepatoma cell

line BEL7402.

med research follows.

Mike

Life Sci. 2003 Nov 14;73(26):3363-74.

Related Articles, Links

Lentin, a novel and potent antifungal protein from shitake mushroom

with inhibitory effects on activity of human immunodeficiency virus-1

reverse transcriptase and proliferation of leukemia cells.

Ngai PH, Ng TB.

Department of Biochemistry, Faculty of Medicine, The Chinese

University of Hong Kong, Shatin, New Territories, Hong Kong, China.

From the fruiting bodies of the edible mushroom Lentinus edodes, a

novel protein designated lentin with potent antifungal activity was

isolated. Lentin was unadsorbed on DEAE-cellulose, and adsorbed on

Affi-gel blue gel and Mono S. The N-terminal sequence of lentin

manifested similarity to endoglucanase. Lentin, which had a molecular

mass of 27.5 kDa, inhibited mycelial growth in a variety of fungal

species including Physalospora piricola, Botrytis cinerea and

Mycosphaerella arachidicola. Lentin also exerted an inhibitory

activity on HIV-1 reverse transcriptase and proliferation of leukemia

cells.

PMID: 14572878 [PubMed - indexed for MEDLINE]

Reishi:

Ganoderic acid produced from submerged culture of Ganoderma lucidum

induces cell cycle arrest and cytotoxicity in human hepatoma cell

line BEL7402.

Yang HL.

School of Life & Environmental Science, Wenzhou University, Wenzhou,

People's Republic of China. yanghl999@...

Ganoderic acid (GA), produced by submerged culture of Ganoderma

lucidum, at 500 microg/ml, caused nearly a 70% inhibition of the

growth of human hepatoma cell line BEL7402 but not of a normal human

liver cell line L02. Flow cytometry analyses showed that GA blocked

the BEL7402 cell cycle at the transition from G(1 )to S phase.

PMID: 16086244 [PubMed - indexed for MEDLINE]

Ganoderma lucidum suppresses angiogenesis through the inhibition of

secretion of VEGF and TGF-beta1 from prostate cancer cells.

Stanley G, Harvey K, Slivova V, Jiang J, Sliva D.

Cancer Research Laboratory, Methodist Research Institute, 1800 N

Capitol Ave, E504, Indianapolis, IN 46202, USA.

Ganoderma lucidum (G. lucidum) is a popular medicinal mushroom that

has been used as a home remedy for the general promotion of health

and longevity in East Asia. The dried powder of G. lucidum, which was

recommended as a cancer chemotherapy agent in traditional Chinese

medicine, is currently popularly used worldwide in the form of

dietary supplements. We have previously demonstrated that G. lucidum

induces apoptosis, inhibits cell proliferation, and suppresses cell

migration of highly invasive human prostate cancer cells PC-3.

However, the molecular mechanism(s) responsible for the inhibitory

effects of G. lucidum on the prostate cancer cells has not been fully

elucidated. In the present study, we examined the effect of G.

lucidum on angiogenesis related to prostate cancer. We found that G.

lucidum inhibits the early event in angiogenesis, capillary

morphogenesis of the human aortic endothelial cells. These effects

are caused by the inhibition of constitutively active AP-1 in

prostate cancer cells, resulting in the down-regulation of secretion

of VEGF and TGF-beta1 from PC-3 cells. Thus, G. lucidum modulates the

phosphorylation of Erk1/2 and Akt kinases in PC-3 cells, which in

turn inhibits the activity of AP-1. In summary, our results suggest

that G. lucidum inhibits prostate cancer-dependent angiogenesis by

modulating MAPK and Akt signaling and could have potential

therapeutic use for the treatment of prostate cancer.

PMID: 15781230 [PubMed - indexed for MEDLINE]

Growth inhibition of U937 leukemia cells by aqueous extract of

Cordyceps militaris through induction of apoptosis.

Park C, Hong SH, Lee JY, Kim GY, Choi BT, Lee YT, Park DI, Park YM,

Jeong YK, Choi YH.

Department of Oriental Medicine, Dongeui University College of

Oriental Medicine, Biomedical Research Center of Oriental Medicine,

Busan 614-052, Korea.

Cordyceps militaris is a traditional herbal ingredient, which has

been used for patients suffering from cancer in Oriental medicine. In

the present study, we investigated the biochemical mechanisms of anti-

proliferative effects by aqueous extract of C. militaris (AECM) in

human leukemia U937 cells. It was found that AECM could inhibit cell

growth of U937 cells in a dose-dependent manner, which was associated

with morphological change and apoptotic cell death such as formation

of apoptotic bodies and DNA fragmentation. We observed the down-

regulation of anti-apoptotic Bcl-2 expression and proteolytic

activation of caspase-3 in AECM-treated U937 cells. However, AECM did

not affect the pro-apoptotic Bax expression and activity of caspase-

9. Furthermore, Western blotting and RT-PCR revealed that AECM

treatment caused a dose-dependent inhibition of cyclooxygenase-2 and

prostaglandin E2 accumulation. Taken together, these results

indicated that the anti-proliferative effects of AECM were associated

with the induction of apoptotic cell death through regulation of

several major growth regulatory gene products such as Bcl-2 family

expression and caspase protease activity, and AECM may have

therapeutic potential in human leukemia treatment.

PMID: 15870944 [PubMed - indexed for MEDLINE]