Composition of inflammatory infiltrate and its correlation with HBV/HCV antigen expression

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World J Gastroenterol. 2008 Jul 7;14(25):4040-6.

Composition of inflammatory infiltrate and its correlation with HBV/HCV antigen

expression.

Walewska-Zielecka B, Madalinski K, Jablonska J, Godzik P, Cielecka-Kuszyk J,

Litwinska B.

Laboratory of Viral Immunopathology, Department of Virology, National Institute

of Public Health, National Institute of Hygiene, Warsaw 00-791, Poland.

kmadalinski@....

AIM: To study the composition of liver inflammatory infiltrate in biopsy

material from patients chronically infected with hepatotropic viruses and to

evaluate the correlation of inflammatory infiltrate with hepatitis B virus (HBV)

and hepatitis C virus (HCV) viral antigen expression in chronic B and C

hepatitis. METHODS: The phenotype of inflammatory cells was evaluated by the

EnVision system, using a panel of monoclonal antibodies. HBV and HCV antigens

were detected with the use of monoclonal anti-HBs, polyclonal anti-HBc and

anti-HCV antibodies, respectively. RESULTS: The cellular composition of liver

inflammatory infiltrate was similar in the patients with B and C hepatitis:

~50%-60% of cells were T helper lymphocytes. Approximately 25% were T cytotoxic

lymphocytes; B lymphocytes comprised 15% of inflammatory infiltrate; other

cells, including NK, totalled 10%. Expression of HLA antigens paralleled

inflammatory activity. Portal lymphadenoplasia was found more often in hepatitis

C (54.5%) than in hepatitis B (30.6%). Expression of HBcAg was found more often

in chronic B hepatitis of moderate or severe activity. Overall inflammatory

activity in HBV-infected cases did not correlate with the intensity of HBsAg

expression in hepatocytes. Inflammatory infiltrates accompanied the focal

expression of HCV antigens. A direct correlation between antigen expression and

inflammatory reaction in situ was noted more often in hepatitis C than B.

CONCLUSION: Irrespective of the etiology and activity of hepatitis, components

of the inflammatory infiltrate in liver were similar. Overall inflammatory

activity did not correlate with the expression of HBsAg and HCVAg; HBcAg

expression, however, accompanied chronic hepatitis B of moderate and severe

activity.

PMID: 18609688 [PubMed - in process]

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