Surface antibody and cytokine response to recombinant Chinese hamster ovary cell (CHO) hepatitis B vaccine.

[Guest guest]

Vaccine. 2011 Jun 28. [Epub ahead of print]

Surface antibody and cytokine response to recombinant Chinese hamster ovary cell

(CHO) hepatitis B vaccine.

Zhang W, Han L, Lin C, Wang H, Pang X, Li L, Gao P, Lin H, Gong X, Tang Y, Ma J,

Zhang H, Wang C, Yang P, Li H, Sun M, He X.

Source

Beijing Center for Disease Control and Prevention, Beijing 100013, China.

Abstract

OBJECTIVE:

To compare the immune responses of the 10ìg and 20ìg doses of CHO hepatitis B

vaccine on adults.

METHODS:

Adults aged 18-45 years who gave a history of never having received hepatitis B

vaccine and lacked serologic evidence of infection to hepatitis B virus (HBV)

infection or previous vaccination were enrolled into the study. A total of 642

eligible participants were randomized to receive 3 doses of either the 10ìg or

the 20ìg formulation of CHO hepatitis B vaccine in a 0-1-6 month schedule. Each

study subject had a serologic specimen collected one month following the third

vaccine dose that was tested for markers of HBV infection and anti-HBs by Abbott

I2000. Persons who tested negative for anti-HBs negative persons were tested for

HBV DNA. Logistic regression was used to identify factors associated with

antibody response. Among the participants, 153 subjects had their lymphocytes

cultivated and tested for cytokine production. Enzyme-linked immunospot

(ELISPOT) was used to test spot numbers of IL-4, IFN-ã which produced by

lymphocyte.

RESULTS:

The anti-HBs seroconversion rate was 88.8% (95% CI: 85.4-92.2%) and 95.3% (95%

CI: 93.0-97.6%), respectively in 10ìg and 20ìg group. Geometric mean titers

(GMT) were 173.42mIU/ml and 585.51mIU/ml, respectively in 10ìg and 20ìg groups.

Multivariate analysis demonstrated that diabetes, spouse is hepatitis B virus

infector, older age and receipt of the 10ìg dose were all negatively associated

with antibody response (P<.05). Cellular immunity results showed: IL-4 immunity

spot numbers in 20ìg group was higher than 10ìg group. With anti-HBs increased,

the IL-4 immunity spot numbers increased significantly which had significant

positive correlation (Spearman coefficient=0.538, P<0.0001). IFN-ã spot numbers

had no statistical significant between the two groups.

CONCLUSION:

The humoral immunity and cytokines response among the group that received the

20ìg CHO hepatitis B vaccine dose was superior compared to the group that

received the 10ìg dose. The 20ìg dose of CHO hepatitis B vaccine should be

prioritized for adult vaccination programs in China.

Copyright © 2011. Published by Elsevier Ltd.

PMID: 21722684 [PubMed - as supplied by publisher]