Asialoglycoprotein receptor interacts with the preS1 domain of hepatitis B virus in vivo and in vitro.

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Arch Virol. 2011 Jan 5. [Epub ahead of print]

Asialoglycoprotein receptor interacts with the preS1 domain of hepatitis B virus

in vivo and in vitro.

Zhang X, Lin SM, Chen TY, Liu M, Ye F, Chen YR, Shi L, He YL, Wu LX, Zheng SQ,

Zhao YR, Zhang SL.

Department of Infectious Diseases, The First Affiliated Hospital of Medical

college of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an 710061,

Shaanxi Province, China.

Abstract

BACKGROUND: The preS1 domain of the large envelope protein has been identified

as an essential viral structure involved in hepatitis B virus (HBV) attachment.

However, the cellular receptor(s) for HBV has not yet been identified.

AIMS: To identify a cell-surface receptor for HBV, which could elucidate the

molecular mechanism of HBV infection.

METHODS: A novel yeast two-hybrid system was used to screen proteins interacting

with the preS1 region of HBV. Their interaction was verified by yeast

cotransformation, coimmunoprecipitation and mammalian two-hybrid assay, while

their intracellular and tissue localization was analyzed by confocal microscopy

and immunohistochemistry, respectively.

RESULTS: Asialoglycoprotein receptor (ASGPR) interacted specifically and

directly with the preS1 domain of HBV in vivo and in vitro. The levels of

expression of preS1 and ASGPR in the liver were similar and correlated with each

other.

CONCLUSIONS: ASGPR is a candidate receptor for HBV that mediates further steps

of HBV entry.

PMID: 21207081 [PubMed - as supplied by publisher]