Correlation between gastrointestinal fungi and varying degrees of chronic hepatitis B virus infection

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http://www.dmidjournal.com/article/PIIS0732889310001288/abstract?rss=yes

DIAGNOSTIC MICROBIOLOGY & INFECTIOUS DISEASE

Volume 70, Issue 4, Pages 492-498 (August 2011)

Correlation between gastrointestinal fungi and varying degrees of chronic

hepatitis B virus infection

Yu Chen, Zhenjing Chen, Renyong Guo, Nan Chen, Haifeng Lu, Shuai Huang, Jie

Wang, Lanjuan Li

Received 10 December 2009; accepted 9 April 2010. published online 16 September

2010.

Abstract

This study aims to compare the diversity of intestinal fungal microbiota in

patients with different degrees of chronic hepatitis B virus (HBV) infection.

Culture-independent and culture-dependent methods were performed on 38 patients

with hepatitis B cirrhosis, 35 patients with chronic hepatitis B, 33 HBV

carriers, and 55 healthy volunteers. An overall fungal biodiversity of 37

different operational taxonomic units was found in the clone libraries; only

Candida spp. and Saccharomyces cerevisiae were obtained by the culture-dependent

analysis. There was a higher richness of fungal species in patients with

hepatitis B cirrhosis than in patients with chronic hepatitis B, and the latter

was higher than that in HBV carriers and healthy volunteers. There was little

difference in enteric fungal diversity between HBV carriers and healthy

volunteers. The results indicate that the diversity of enteric fungi was

positively correlated with the disease progression of patients with different

degrees of chronic HBV infection.

Keywords: 18S rDNA, Culture-independent method, Diversity, Intestinal fungi, HBV

infection

State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First

Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou,

Zhejiang 310003, PR China

Corresponding author. Tel.: +86-571-87236458; fax: +86-571-87236459.

PII: S0732-8893(10)00128-8

doi:10.1016/j.diagmicrobio.2010.04.005