The novel use of a routine quantitative system to analyze the activity, content and affinity of an antibody to hepatitis B core antigen

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http://www.journalofclinicalvirology.com/article/PIIS1386653211003659/abstract?r\

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The novel use of a routine quantitative system to analyze the activity, content

and affinity of an antibody to hepatitis B core antigen

Yanan Han, Bo Wang, Hui Liu

Received 19 July 2011; received in revised form 23 July 2011; accepted 5

September 2011. published online 05 October 2011.

Corrected Proof

Abstract

Background

Currently, quantitative measurement of serum markers of HBV infection has been

widely used, but commonly used analysis for specific antibodies only involves

the measurement of the total antibody activity, and the binding affinity or

protein content is rarely analyzed.

Objective

To investigate the detailed features of an antibody to hepatitis B core antigen

(anti-HBc) during different periods of hepatitis B virus (HBV) infection with a

new method of analysis.

Study design

Serum samples were collected from patients that were positive for the anti-HBc

antibody. On the basis of the other serological markers in the samples, all

patients were divided into a hepatitis B surface antigen (HBsAg)-positive group

and an antibody to hepatitis B surface antigen (anti-HBs)-positive group. All

samples were diluted 2-, 20- and 200-fold. Anti-HBc quantification was measured

with a chemiluminescent microparticle immunoassay; total anti-HBc activity,

protein content and affinity were calculated according to a measured value of

each dilution. Serum HBV DNA load and alanine aminotransferase (ALT) levels were

also measured.

Results

The total anti-HBc activity in the HBsAg-positive group was statistically higher

than that in the anti-HBs-positive group (p<0.05). The anti-HBc protein content

during active HBV infection was statistically higher than during the

convalescence stage (p<0.05), while anti-HBc affinity during HBV infection was

lower than during recovery. There were correlations among total activity,

affinity, protein content of anti-HBc, and ALT, HBV-DNA (p<0.05).

Conclusions

It is potentially possible to predict the status of HBV infection by measuring

total activity, protein content and affinity of anti-HBc.

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The novel use of a routine quantitative system to analyze the activity, content

and affinity of an antibody to hepatitis B core antigen

Journal of Clinical Virology, 10/07/2011

Han Y et al. – It is potentially possible to predict the status of hepatitis B

virus (HBV) infection by measuring total activity, protein content and affinity

of antibody to hepatitis B core antigen (anti–HBc).

Methods

• Serum samples were collected from patients that were positive for the anti–HBc

antibody.

• On the basis of the other serological markers in the samples, all patients

were divided into a hepatitis B surface antigen (HBsAg)–positive group and an

antibody to hepatitis B surface antigen (anti–HBs)–positive group.

• All samples were diluted 2–, 20– and 200–fold. Anti–HBc quantification was

measured with a chemiluminescent microparticle immunoassay; total anti–HBc

activity, protein content and affinity were calculated according to a measured

value of each dilution.

• Serum HBV DNA load and alanine aminotransferase (ALT) levels were also

measured.

Results

• Total anti–HBc activity in the HBsAg–positive group was statistically higher

than that in the anti–HBs–positive group (p<0.05).

• Anti–HBc protein content during active HBV infection was statistically higher

than during the convalescence stage (p<0.05), while anti–HBc affinity during HBV

infection was lower than during recovery.

• There were correlations among total activity, affinity, protein content of

anti–HBc, and ALT, HBV–DNA (p<0.05).