Influence of mutation of the HFE gene on the progression of chronic viral hepatitis B and C in Moroccan patients.

[Guest guest]

J Med Virol. 2011 Dec;83(12):2096-102. doi: 10.1002/jmv.22245.

Influence of mutation of the HFE gene on the progression of chronic viral

hepatitis B and C in Moroccan patients.

Ezzikouri S, Rebbani K, Mostafa A, El Feydi AE, Afifi R, Brahim I, Kitab B,

Benazzouz M, Kandil M, Nadifi S, Pineau P, Benjelloun S.

Source

Viral Hepatitis Laboratory, Pasteur Institute of Morocco, Casablanca, Morocco.

sayeh.ezzikouri@....

Abstract

The implication of hemochromatosis (HFE) gene mutations in chronic viral

hepatitis remains controversial. The aim of the present study was to measure the

frequencies of the common HFE gene mutations in Moroccan subjects with chronic

viral hepatitis B and C and to assess their influence on the progression of

liver disease. H63D and C282Y mutations were screened by the polymerase chain

reaction followed by restriction fragment length polymorphism analysis in 170

chronic hepatitis B patients, 168 chronic hepatitis C patients, and 200 healthy

controls. A very small proportion of patients infected with hepatitis B virus

(HBV) or hepatitis C virus (HCV; 1.8% and none, respectively) were heterozygous

for the C282Y mutation, that is, rates not statistically different from those

observed in healthy control (2%, P > 0.05). Similarly, the frequency of the

H63D allele was not significantly different between HBV (13.8%) or HCV (14.3%)

patients and controls (13.5%, P > 0.05). Multivariate analysis showed that

carriers of the H63D mutation infected with HBV are at higher risk to progress

towards an advanced liver disease when compared with patients infected with HBV

with wild-type (OR = 2.45, 95% CI = 1.07-5.58). In contrast, no

association between HFE mutated HCV-infected patients and an increased risk of

disease progression was found (OR = 1.24, 95% CI = 0.61-2.50,

P = 0.547). In conclusion, in Morocco the frequency of the HFE C282Y allele

is very low and H63D mutation carriage occurs in almost 14% of the patients, a

rate similar in chronic hepatitis patients and healthy controls. In the case of

chronic hepatitis B, the carriage of the H63D variant represents a risk factor

of evolution towards a more active disease. J. Med. Virol. 83:2096-2102, 2011.

© 2011 Wiley Periodicals, Inc.

Copyright © 2011 Wiley Periodicals, Inc.

PMID: 22012716 [PubMed - in process]

[Guest guest]

J Med Virol. 2011 Dec;83(12):2096-102. doi: 10.1002/jmv.22245.

Influence of mutation of the HFE gene on the progression of chronic viral

hepatitis B and C in Moroccan patients.

Ezzikouri S, Rebbani K, Mostafa A, El Feydi AE, Afifi R, Brahim I, Kitab B,

Benazzouz M, Kandil M, Nadifi S, Pineau P, Benjelloun S.

Source

Viral Hepatitis Laboratory, Pasteur Institute of Morocco, Casablanca, Morocco.

sayeh.ezzikouri@....

Abstract

The implication of hemochromatosis (HFE) gene mutations in chronic viral

hepatitis remains controversial. The aim of the present study was to measure the

frequencies of the common HFE gene mutations in Moroccan subjects with chronic

viral hepatitis B and C and to assess their influence on the progression of

liver disease. H63D and C282Y mutations were screened by the polymerase chain

reaction followed by restriction fragment length polymorphism analysis in 170

chronic hepatitis B patients, 168 chronic hepatitis C patients, and 200 healthy

controls. A very small proportion of patients infected with hepatitis B virus

(HBV) or hepatitis C virus (HCV; 1.8% and none, respectively) were heterozygous

for the C282Y mutation, that is, rates not statistically different from those

observed in healthy control (2%, P > 0.05). Similarly, the frequency of the

H63D allele was not significantly different between HBV (13.8%) or HCV (14.3%)

patients and controls (13.5%, P > 0.05). Multivariate analysis showed that

carriers of the H63D mutation infected with HBV are at higher risk to progress

towards an advanced liver disease when compared with patients infected with HBV

with wild-type (OR = 2.45, 95% CI = 1.07-5.58). In contrast, no

association between HFE mutated HCV-infected patients and an increased risk of

disease progression was found (OR = 1.24, 95% CI = 0.61-2.50,

P = 0.547). In conclusion, in Morocco the frequency of the HFE C282Y allele

is very low and H63D mutation carriage occurs in almost 14% of the patients, a

rate similar in chronic hepatitis patients and healthy controls. In the case of

chronic hepatitis B, the carriage of the H63D variant represents a risk factor

of evolution towards a more active disease. J. Med. Virol. 83:2096-2102, 2011.

© 2011 Wiley Periodicals, Inc.

Copyright © 2011 Wiley Periodicals, Inc.

PMID: 22012716 [PubMed - in process]