Inactivation and Survival of Hepatitis C Virus on Inanimate Surfaces

[Guest guest]

J Infect Dis. 2011 Oct 19. [Epub ahead of print]

Inactivation and Survival of Hepatitis C Virus on Inanimate Surfaces.

Doerrbecker J, Friesland M, Ciesek S, hsen TJ, Mateu-Gelabert P, Steinmann

J, Steinmann J, Pietschmann T, Steinmann E.

Source

Division of Experimental Virology, Twincore, Centre for Experimental and

Clinical, Infection Research, a joint venture between the Medical School

Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI).

Abstract

Background. Hepatitis C virus (HCV) cross-contamination from inanimate

surfaces or objects has been implicated in transmission of HCV in health-care

settings and among injection drug users. We established HCV-based carrier and

drug transmission assays that simulate practical conditions to study

inactivation and survival of HCV on inanimate surfaces.Methods. Studies were

performed with authentic cell culture derived viruses. HCV was dried on steel

discs and biocides were tested for their virucidal efficacy against HCV.

Infectivity was determined by a limiting dilution assay. HCV stability was

analyzed in a carrier assay for several days or in a drug transmission assay

using a spoon as cooker.Results. HCV can be dried and recovered efficiently in

the carrier assay. The most effective alcohol to inactivate the virus was

1-propanol, and commercially available disinfectants reduced infectivity of HCV

to undetectable levels. Viral infectivity on inanimate surfaces was detectable

in the presence of serum for up to 5 days, and temperatures of about 65-70°C

were required to eliminate infectivity in the drug transmission

assay.Conclusions. These findings are important for assessment of HCV

transmission risks and should facilitate the definition of stringent public

health interventions to prevent HCV infections.

PMID: 22013220 [PubMed - as supplied by publisher

[Guest guest]

J Infect Dis. 2011 Oct 19. [Epub ahead of print]

Inactivation and Survival of Hepatitis C Virus on Inanimate Surfaces.

Doerrbecker J, Friesland M, Ciesek S, hsen TJ, Mateu-Gelabert P, Steinmann

J, Steinmann J, Pietschmann T, Steinmann E.

Source

Division of Experimental Virology, Twincore, Centre for Experimental and

Clinical, Infection Research, a joint venture between the Medical School

Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI).

Abstract

Background. Hepatitis C virus (HCV) cross-contamination from inanimate

surfaces or objects has been implicated in transmission of HCV in health-care

settings and among injection drug users. We established HCV-based carrier and

drug transmission assays that simulate practical conditions to study

inactivation and survival of HCV on inanimate surfaces.Methods. Studies were

performed with authentic cell culture derived viruses. HCV was dried on steel

discs and biocides were tested for their virucidal efficacy against HCV.

Infectivity was determined by a limiting dilution assay. HCV stability was

analyzed in a carrier assay for several days or in a drug transmission assay

using a spoon as cooker.Results. HCV can be dried and recovered efficiently in

the carrier assay. The most effective alcohol to inactivate the virus was

1-propanol, and commercially available disinfectants reduced infectivity of HCV

to undetectable levels. Viral infectivity on inanimate surfaces was detectable

in the presence of serum for up to 5 days, and temperatures of about 65-70°C

were required to eliminate infectivity in the drug transmission

assay.Conclusions. These findings are important for assessment of HCV

transmission risks and should facilitate the definition of stringent public

health interventions to prevent HCV infections.

PMID: 22013220 [PubMed - as supplied by publisher