Evaluation of dual priming oligonucleotide-based multiplex PCR for detection of HBV YMDD mutants

[Guest guest]

Arch Virol. 2008;153(11):2019-25. Epub 2008 Oct 5.

Evaluation of dual priming oligonucleotide-based multiplex PCR for detection of

HBV YMDD mutants.

Woo HY, Park H, Kim BI, Jeon WK, Kim YJ.

Department of Laboratory Medicine, Kangbuk Samsung Hospital, Sungkyunkwan

University School of Medicine, #108 Pyeong-dong, Jongno-gu, Seoul, South Korea.

We evaluated the usefulness of dual priming oligonucleotide (DPO)-based

multiplex PCR, Seeplex HBV Lami-DR assay (Seegene Institute of Life Sciences,

Seoul, Korea), to detect lamivudine-resistant HBV mutants in a comparison with

the use of TRUGENE HBV genotyping and restriction fragment mass polymorphism

(RFMP). Sera from 44 chronic hepatitis B patients were analyzed for the presence

of mutations at codons 180 and 204 by performing DPO-based multiplex PCR, RFMP,

and TRUGENE. The overall concordance rate among the three assays was 40.9%

(18/44). Concordance rates between multiplex PCR and RFMP or multiplex PCR and

TRUGENE were 61.4% (27/44) and 50.0% (22/44), respectively. In ten patients,

multiplex PCR identified additional mutants not found using the other two

methods. DPO-based multiplex PCR is a highly sensitive method to identify minor

mutant populations and could be a practical tool in the monitoring of lamivudine

resistance.

PMID: 18836856 [PubMed - indexed for MEDLINE

[Guest guest]

Arch Virol. 2008;153(11):2019-25. Epub 2008 Oct 5.

Evaluation of dual priming oligonucleotide-based multiplex PCR for detection of

HBV YMDD mutants.

Woo HY, Park H, Kim BI, Jeon WK, Kim YJ.

Department of Laboratory Medicine, Kangbuk Samsung Hospital, Sungkyunkwan

University School of Medicine, #108 Pyeong-dong, Jongno-gu, Seoul, South Korea.

We evaluated the usefulness of dual priming oligonucleotide (DPO)-based

multiplex PCR, Seeplex HBV Lami-DR assay (Seegene Institute of Life Sciences,

Seoul, Korea), to detect lamivudine-resistant HBV mutants in a comparison with

the use of TRUGENE HBV genotyping and restriction fragment mass polymorphism

(RFMP). Sera from 44 chronic hepatitis B patients were analyzed for the presence

of mutations at codons 180 and 204 by performing DPO-based multiplex PCR, RFMP,

and TRUGENE. The overall concordance rate among the three assays was 40.9%

(18/44). Concordance rates between multiplex PCR and RFMP or multiplex PCR and

TRUGENE were 61.4% (27/44) and 50.0% (22/44), respectively. In ten patients,

multiplex PCR identified additional mutants not found using the other two

methods. DPO-based multiplex PCR is a highly sensitive method to identify minor

mutant populations and could be a practical tool in the monitoring of lamivudine

resistance.

PMID: 18836856 [PubMed - indexed for MEDLINE