Evaluation of peptide nucleic acid array for the detection of hepatitis B virus mutations associated with antiviral resistance.

June 3, 2011

Arch Virol. 2011 May 19. [Epub ahead of print]

Evaluation of peptide nucleic acid array for the detection of hepatitis B virus

mutations associated with antiviral resistance.

Choi YJ, Kim HS, Lee SH, Park JS, Nam HS, Kim HJ, Kim CJ, Jeong DJ, Park KS,

Baek KA.

Source

Department of Laboratory Medicine, Cheonan Hospital, Soonchunhyang University,

23-20, Bongmyung-dong, Cheonan-si, South Korea, clinpath@....

Abstract

A major problem of long-term antiviral therapy in chronic hepatitis B patients

is the emergence of hepatitis B virus (HBV) mutations associated with drug

resistance. Recently, a new array using peptide nucleic acids (PNAs), which are

synthetic nucleic acid analogues, was developed for the detection of HBV

mutations at six different codon positions associated with lamivudine (LAM) and

adefovir (ADV) resistance. We compared the PNA array with direct sequencing and

reverse hybridization (INNO-LiPA) in 73 samples obtained from chronic hepatitis

B patients. The PNA array detected mutations associated with LAM and/or ADV

resistance in 60 (82.2%) of the 73 samples. The overall concordance rate of PNA

array and INNO-LiPA compared with direct sequencing was 99.5% and 98.2%,

respectively. The rate of complete concordance between PNA array and INNO-LiPA

was 92.7%. The PNA array assay results were comparable with INNO-LiPA for

detection of HBV mutations associated with antiviral resistance.

PMID: 21594598 [PubMed - as supplied by publisher]

June 3, 2011