HBsAg-negative mono-infection with hepatitis B virus genotype G

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http://www.ingentaconnect.com/content/bsc/jvh/2011/00000018/00000011/art00008

HBsAg-negative mono-infection with hepatitis B virus genotype G

Authors: Zaaijer, H. L.; Boot, H. J.1; van Swieten, P.2; Koppelman, M. H. G.

M.3; Cuypers, H. T. M.2

Source: Journal of Viral Hepatitis, Volume 18, Number 11, 1 November 2011 , pp.

815-819(5)

Publisher: Wiley-Blackwell

Abstract:

Summary.  Infection with a genotype G strain of hepatitis B virus (HBV-G)

often occurs as a co-infection with HBV genotype A. In mono-infection with

HBV-G, the production of hepatitis B surface antigen (HBsAg), HBe antigen and

anti-HBe seems diminished, hampering the serological diagnosis of HBV-G

mono-infection. To corroborate this notion, we studied in detail a series of

samples of a blood donor with transient HBV-G infection. In this donor, during

the temporary presence of HBV DNA and the seroconversion to HBcore antibodies

(anti-HBc), no HBsAg or hepatitis B e antigen was detected. During follow-up, no

anti-HBe appeared. Multiple resistance mutations to lamivudine were present,

demonstrating primary infection with a resistant HBV strain. Cloning and

sequencing indicated that no other HBV genotype but genotype G was present. Like

other HBV-G isolates, the DNA sequence of the HBsAg a-determinant showed no

mutations that could explain the failure to detect HBsAg. Our findings

demonstrate that HBV genotype G mono-infection occurs and that routine serology

is unsuitable for its detection.

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1365-2893.2010.01397.x

Affiliations:1: Laboratory for Infectious Disease and Screening, National

Institute for Public Health and the Environment (RIVM), Bilthoven 2: Virology

Department, Sanquin, Amsterdam 3: National Screening Laboratory, Sanquin,

Amsterdam, the Netherlands

Publication date: 2011-11-01

[Guest guest]

http://www.ingentaconnect.com/content/bsc/jvh/2011/00000018/00000011/art00008

HBsAg-negative mono-infection with hepatitis B virus genotype G

Authors: Zaaijer, H. L.; Boot, H. J.1; van Swieten, P.2; Koppelman, M. H. G.

M.3; Cuypers, H. T. M.2

Source: Journal of Viral Hepatitis, Volume 18, Number 11, 1 November 2011 , pp.

815-819(5)

Publisher: Wiley-Blackwell

Abstract:

Summary.  Infection with a genotype G strain of hepatitis B virus (HBV-G)

often occurs as a co-infection with HBV genotype A. In mono-infection with

HBV-G, the production of hepatitis B surface antigen (HBsAg), HBe antigen and

anti-HBe seems diminished, hampering the serological diagnosis of HBV-G

mono-infection. To corroborate this notion, we studied in detail a series of

samples of a blood donor with transient HBV-G infection. In this donor, during

the temporary presence of HBV DNA and the seroconversion to HBcore antibodies

(anti-HBc), no HBsAg or hepatitis B e antigen was detected. During follow-up, no

anti-HBe appeared. Multiple resistance mutations to lamivudine were present,

demonstrating primary infection with a resistant HBV strain. Cloning and

sequencing indicated that no other HBV genotype but genotype G was present. Like

other HBV-G isolates, the DNA sequence of the HBsAg a-determinant showed no

mutations that could explain the failure to detect HBsAg. Our findings

demonstrate that HBV genotype G mono-infection occurs and that routine serology

is unsuitable for its detection.

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1365-2893.2010.01397.x

Affiliations:1: Laboratory for Infectious Disease and Screening, National

Institute for Public Health and the Environment (RIVM), Bilthoven 2: Virology

Department, Sanquin, Amsterdam 3: National Screening Laboratory, Sanquin,

Amsterdam, the Netherlands

Publication date: 2011-11-01