Low prevalence of hepatitis B virus pre-s deletion mutation in Indonesia.

[Guest guest]

J Med Virol. 2011 Oct;83(10):1717-26. doi: 10.1002/jmv.22172.

Low prevalence of hepatitis B virus pre-s deletion mutation in Indonesia.

Utama A, Siburian MD, Fanany I, Intan MD, Dhenni R, Kurniasih TS, Lelosutan SA,

Achwan WA, Arnelis, Lukito B, Yusuf I, Lesmana LA, Sulaiman A, Tai S.

Source

Molecular Epidemiology Division, Mochtar Riady Institute for Nanotechnology,

Universitas Pelita Harapan, Lippo Karawaci, Tangerang, Indonesia.

autama@....

Abstract

The molecular epidemiological study of hepatitis B virus (HBV) in Indonesia is

still limited. This study was aimed to identify the prevalence of HBV pre-S

deletion/insertion mutations, and to assess the association of pre-S deletion

mutation with liver disease progression in Indonesia. Pre-S mutations were

identified by direct sequencing. Of the 265 subjects, 32 samples (12.1%)

harbored pre-S deletion/insertion mutations. The prevalence of those pre-S

mutations was 2.7% (2/75), 12.9% (8/62), 16.7% (11/66), and 17.7% (11/62) in

asymptomatic carrier, chronic hepatitis, liver cirrhosis, and hepatocellular

carcinoma groups, respectively. Statistical analysis showed significant

difference among them (P = 0.024). In HBV genotype B (HBV/B), pre-S1,

pre-S1/S2, and pre-S2 deletion mutations were detected respectively in 3

(17.6%), 4 (23.5%), and 9 (52.9%) of 17 samples. On the other hand, in HBV/C, 12

of 15 samples (80.0%) showed a pre-S2 deletion mutation, and only 2 samples

(13.3%) demonstrated a pre-S1/S2 deletion mutation. These results suggest that

in HBV/B deletion mutation tends to occur in pre-S1 or pre-S1/S2 region, while

in HBV/C the deletion mutation usually occurs in the pre-S2 region. Analysis of

complete genome of four viruses confirmed that 3 isolates were classified into

HBV/B3, and 1 isolate was HBV/C1. However, SimPlot and BootScan analyses showed

that isolate 08.10.002 was an intragenotypic recombinant between HBV/B3 and

HBV/B4. As conclusion, the prevalence of HBV pre-S mutations was relatively low

in Indonesian patients compared to those from Taiwan, Japan, and other Asian

countries. There was a weak association between pre-S deletion mutation and

progressive liver disease. J. Med. Virol. 83:1717-1726, 2011. © 2011

Wiley-Liss, Inc.

Copyright © 2011 Wiley-Liss, Inc.

PMID: 21837787 [PubMed - in process]

[Guest guest]

J Med Virol. 2011 Oct;83(10):1717-26. doi: 10.1002/jmv.22172.

Low prevalence of hepatitis B virus pre-s deletion mutation in Indonesia.

Utama A, Siburian MD, Fanany I, Intan MD, Dhenni R, Kurniasih TS, Lelosutan SA,

Achwan WA, Arnelis, Lukito B, Yusuf I, Lesmana LA, Sulaiman A, Tai S.

Source

Molecular Epidemiology Division, Mochtar Riady Institute for Nanotechnology,

Universitas Pelita Harapan, Lippo Karawaci, Tangerang, Indonesia.

autama@....

Abstract

The molecular epidemiological study of hepatitis B virus (HBV) in Indonesia is

still limited. This study was aimed to identify the prevalence of HBV pre-S

deletion/insertion mutations, and to assess the association of pre-S deletion

mutation with liver disease progression in Indonesia. Pre-S mutations were

identified by direct sequencing. Of the 265 subjects, 32 samples (12.1%)

harbored pre-S deletion/insertion mutations. The prevalence of those pre-S

mutations was 2.7% (2/75), 12.9% (8/62), 16.7% (11/66), and 17.7% (11/62) in

asymptomatic carrier, chronic hepatitis, liver cirrhosis, and hepatocellular

carcinoma groups, respectively. Statistical analysis showed significant

difference among them (P = 0.024). In HBV genotype B (HBV/B), pre-S1,

pre-S1/S2, and pre-S2 deletion mutations were detected respectively in 3

(17.6%), 4 (23.5%), and 9 (52.9%) of 17 samples. On the other hand, in HBV/C, 12

of 15 samples (80.0%) showed a pre-S2 deletion mutation, and only 2 samples

(13.3%) demonstrated a pre-S1/S2 deletion mutation. These results suggest that

in HBV/B deletion mutation tends to occur in pre-S1 or pre-S1/S2 region, while

in HBV/C the deletion mutation usually occurs in the pre-S2 region. Analysis of

complete genome of four viruses confirmed that 3 isolates were classified into

HBV/B3, and 1 isolate was HBV/C1. However, SimPlot and BootScan analyses showed

that isolate 08.10.002 was an intragenotypic recombinant between HBV/B3 and

HBV/B4. As conclusion, the prevalence of HBV pre-S mutations was relatively low

in Indonesian patients compared to those from Taiwan, Japan, and other Asian

countries. There was a weak association between pre-S deletion mutation and

progressive liver disease. J. Med. Virol. 83:1717-1726, 2011. © 2011

Wiley-Liss, Inc.

Copyright © 2011 Wiley-Liss, Inc.

PMID: 21837787 [PubMed - in process]