Cohort effect of HCV infection in liver cirrhosis assessed by a 25 year study

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Posted 5/24/00

J Clin Virol 2000 Jun 1;17(1):51-56

Cohort effect of HCV infection in liver cirrhosis assessed by a 25 year

study.

Stanta G, Croce LS, Bonin S, Tisminetzky SG, Baralle FE, Tiribelli C

Istituto di Anatomia Patologica, University of Trieste, 34100, Trieste,

Italy

[Record supplied by publisher]

Background: The role of HCV infection in the development of chronic liver

disease is still unclear. Objectives: Assess the presence of HCV infection

in

patients with liver cirrhosis. Study design: 123 cases of cirrhotic liver

randomly selected over a 25 years period (1969-1994) from the autopsy

archives of the Pathology Department of the University of Trieste, Italy,

were analyzed for the presence of HCV viral genome. Methods: Total RNA was

extracted from formalin-fixed paraffin-embedded tissues of the cirrhotic

liver. Genotype analysis for HCV was performed after RT-PCR by dot-blot

hybridization with the three major genotype-specific probes (G1, G2 and G3).

Results: The overall HCV genome frequency was 50.4% (62/123). The positivity

was quite constant in the 1969-1979 period (35-38%), rose to 65% in 1984,

peaked to 77% in 1989 (P<0.005 vs. the previous decade), and decreased to

50%

in 1994. HCV genotype G1 was found in 89% of the 62 positive samples. The

mean age of death of HCV-positive and HCV-negative patients was comparable

(69+/-12 vs. 67+/-16 years, NS). Conclusions: These data show an increasing

frequency of HCV infection in cirrhotic liver tissues from 1969 to 1994,

which peaked in 1989. The genotype G1 was the almost uniquely associated

with

cirrhosis. These findings indicate that the HCV infection occurred around

the

late 1950s-early 1960s, thus supporting the hypothesis of a cohort effect.

HCV infection seems not to alter the natural history of liver cirrhosis as

indicated by the comparable age at death of HCV positive and HCV negative

patients.

PMID: 10814939

________________________________________________________________________

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[Guest guest]

Posted 5/24/00

J Clin Virol 2000 Jun 1;17(1):51-56

Cohort effect of HCV infection in liver cirrhosis assessed by a 25 year

study.

Stanta G, Croce LS, Bonin S, Tisminetzky SG, Baralle FE, Tiribelli C

Istituto di Anatomia Patologica, University of Trieste, 34100, Trieste,

Italy

[Record supplied by publisher]

Background: The role of HCV infection in the development of chronic liver

disease is still unclear. Objectives: Assess the presence of HCV infection

in

patients with liver cirrhosis. Study design: 123 cases of cirrhotic liver

randomly selected over a 25 years period (1969-1994) from the autopsy

archives of the Pathology Department of the University of Trieste, Italy,

were analyzed for the presence of HCV viral genome. Methods: Total RNA was

extracted from formalin-fixed paraffin-embedded tissues of the cirrhotic

liver. Genotype analysis for HCV was performed after RT-PCR by dot-blot

hybridization with the three major genotype-specific probes (G1, G2 and G3).

Results: The overall HCV genome frequency was 50.4% (62/123). The positivity

was quite constant in the 1969-1979 period (35-38%), rose to 65% in 1984,

peaked to 77% in 1989 (P<0.005 vs. the previous decade), and decreased to

50%

in 1994. HCV genotype G1 was found in 89% of the 62 positive samples. The

mean age of death of HCV-positive and HCV-negative patients was comparable

(69+/-12 vs. 67+/-16 years, NS). Conclusions: These data show an increasing

frequency of HCV infection in cirrhotic liver tissues from 1969 to 1994,

which peaked in 1989. The genotype G1 was the almost uniquely associated

with

cirrhosis. These findings indicate that the HCV infection occurred around

the

late 1950s-early 1960s, thus supporting the hypothesis of a cohort effect.

HCV infection seems not to alter the natural history of liver cirrhosis as

indicated by the comparable age at death of HCV positive and HCV negative

patients.

PMID: 10814939

________________________________________________________________________

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