Anti-CD81 antibodies can prevent a hepatitis C virus infection in vivo

[Guest guest]

Hepatology. 2008 Jul 28;48(6):1761-1768. [Epub ahead of print]

Anti-CD81 antibodies can prevent a hepatitis C virus infection in vivo.

Meuleman P, Hesselgesser J, son M, Vanwolleghem T, Desombere I, Reiser H,

Leroux-Roels G.

Center for Vaccinology, Ghent University and Hospital, Ghent, Belgium.

The viral life cycle of the hepatitis C virus (HCV) has been studied mainly

using different in vitro cell culture models. Studies using pseudoviral

particles (HCVpp) and more recently cell culture-derived virus (HCVcc) suggest

that at least three host cell molecules are important for HCV entry in vitro:

the tetraspanin CD81, the scavenger receptor class B member I, and the tight

junction protein Claudin-1. Whether these receptors are equally important for an

in vivo infection remains to be demonstrated. We show that CD81 is indispensable

for an authentic in vivo HCV infection. Prophylactic treatment with anti-CD81

antibodies completely protected human liver-uPA-SCID mice from a subsequent

challenge with HCV consensus strains of different genotypes. Administration of

anti-CD81 antibodies after viral challenge had no effect. Conclusion: Our

experiments provide evidence for the critical role of CD81 in a genuine HCV

infection in vivo and open new perspectives for the prevention of allograft

reinfection after orthotopic liver transplantation in chronically infected HCV

patients.

(HEPATOLOGY 2008;48:1761-1768.).

PMID: 19030166 [PubMed - as supplied by publisher]

[Guest guest]

Hepatology. 2008 Jul 28;48(6):1761-1768. [Epub ahead of print]

Anti-CD81 antibodies can prevent a hepatitis C virus infection in vivo.

Meuleman P, Hesselgesser J, son M, Vanwolleghem T, Desombere I, Reiser H,

Leroux-Roels G.

Center for Vaccinology, Ghent University and Hospital, Ghent, Belgium.

The viral life cycle of the hepatitis C virus (HCV) has been studied mainly

using different in vitro cell culture models. Studies using pseudoviral

particles (HCVpp) and more recently cell culture-derived virus (HCVcc) suggest

that at least three host cell molecules are important for HCV entry in vitro:

the tetraspanin CD81, the scavenger receptor class B member I, and the tight

junction protein Claudin-1. Whether these receptors are equally important for an

in vivo infection remains to be demonstrated. We show that CD81 is indispensable

for an authentic in vivo HCV infection. Prophylactic treatment with anti-CD81

antibodies completely protected human liver-uPA-SCID mice from a subsequent

challenge with HCV consensus strains of different genotypes. Administration of

anti-CD81 antibodies after viral challenge had no effect. Conclusion: Our

experiments provide evidence for the critical role of CD81 in a genuine HCV

infection in vivo and open new perspectives for the prevention of allograft

reinfection after orthotopic liver transplantation in chronically infected HCV

patients.

(HEPATOLOGY 2008;48:1761-1768.).

PMID: 19030166 [PubMed - as supplied by publisher]