Enchanced levels of Apolipoprotein M during HBV infection feedback suppresses HBV replication

[Guest guest]

http://www.lipidworld.com/content/10/1/154/abstract

Enchanced levels of Apolipoprotein M during HBV infection feedback suppresses

HBV replication

Jin-gang Gu, Cheng-liang Zhu, Duo-zhi Cheng, Yan Xie, Fang Liu and Xin Zhou

For all author emails, please log on.

Lipids in Health and Disease 2011, 10:154 doi:10.1186/1476-511X-10-154

Published: 29 August 2011

Abstract (provisional)

Background

Chronic liver diseases can interfere with hepatic metabolism of lipoproteins,

apolipoproteins. Hepatitis B virus (HBV) is a major etiological agent causing

acute and chronic liver diseases. Apolipoprotein M (ApoM) is a high-density

lipoprotein (HDL) apolipoprotein and exclusively expressed in the liver

parenchyma cells and in the tubular cells of the kidney. This study was to

determine the correlation between HBV infection and ApoM expression. Materials

and Methods: Serum ApoM levels in patients with HBV infection and in healthy

individuals were measured by ELISA, ApoM mRNA expression were determined by

RT-PCR, and the expression of S and E proteins of HBV, as well as the synthesis

of viral DNA were measured by ELISA and real-time PCR.

Results

The levels of serum ApoM was significantly elevated in patients as compared to

healthy individuals (P < 0.001), ApoM promoter activity, mRNA and protein

expression were all stimulated in cells transfected with infectious HBV clone.

In addition, ApoM decreases the expression of S and E proteins of HBV and the

synthesis of viral DNA.

Conclusion

Raised ApoM levels in HBV infection may in turn suppress HBV replication, one of

the protective mechanisms of nature.

The complete article is available as a provisional PDF. The fully formatted PDF

and HTML versions are in production.

[Guest guest]

http://www.lipidworld.com/content/10/1/154/abstract

Enchanced levels of Apolipoprotein M during HBV infection feedback suppresses

HBV replication

Jin-gang Gu, Cheng-liang Zhu, Duo-zhi Cheng, Yan Xie, Fang Liu and Xin Zhou

For all author emails, please log on.

Lipids in Health and Disease 2011, 10:154 doi:10.1186/1476-511X-10-154

Published: 29 August 2011

Abstract (provisional)

Background

Chronic liver diseases can interfere with hepatic metabolism of lipoproteins,

apolipoproteins. Hepatitis B virus (HBV) is a major etiological agent causing

acute and chronic liver diseases. Apolipoprotein M (ApoM) is a high-density

lipoprotein (HDL) apolipoprotein and exclusively expressed in the liver

parenchyma cells and in the tubular cells of the kidney. This study was to

determine the correlation between HBV infection and ApoM expression. Materials

and Methods: Serum ApoM levels in patients with HBV infection and in healthy

individuals were measured by ELISA, ApoM mRNA expression were determined by

RT-PCR, and the expression of S and E proteins of HBV, as well as the synthesis

of viral DNA were measured by ELISA and real-time PCR.

Results

The levels of serum ApoM was significantly elevated in patients as compared to

healthy individuals (P < 0.001), ApoM promoter activity, mRNA and protein

expression were all stimulated in cells transfected with infectious HBV clone.

In addition, ApoM decreases the expression of S and E proteins of HBV and the

synthesis of viral DNA.

Conclusion

Raised ApoM levels in HBV infection may in turn suppress HBV replication, one of

the protective mechanisms of nature.

The complete article is available as a provisional PDF. The fully formatted PDF

and HTML versions are in production.