Mutations in hepatitis B virus DNA from patients with coexisting HBsAg and anti-HBs

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http://www.journalofclinicalvirology.com/article/PIIS1386653211002861/abstract?r\

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Journal of Clinical Virology

Article in Press

Mutations in hepatitis B virus DNA from patients with coexisting HBsAg and

anti-HBs

Yu Chen, Fuchu Qian, Quan Yuan, Xuefen Li, Wei Wu, Xichao Guo, Lanjuan Li

Received 25 February 2011; received in revised form 6 May 2011; accepted 8 July

2011. published online 16 August 2011.

Corrected Proof

Abstract

Background

The serological markers with coexistence of hepatitis B surface antigen (HBsAg)

and antibody to HBsAg (anti-HBs) of hepatitis B virus (HBV) infection were rare

pattern. The virological significance, immune response and clinical outcome of

these patients remain largely unknown.

Objectives

This research explores the relationship between this serological profile and HBV

genome variants.

Study design

We studied 35 patients both carrying HBsAg and anti-HBs (group I), and 70

patients with HBsAg positive but anti-HBs negative (group II, served as

control). The HBV genome sequences were obtained by direct sequencing of

polymerase chain reaction (PCR) products.

Results

The amino acid (aa) variation within major hydrophilic region (MHR), especially

in the first loop (aa124-137) of “a” determinant in group I is significantly

higher than those in group II. The aa variation of cytotoxic lymphocyte (CTL)

epitope in HBsAg (aa87–aa95) in group I is also significantly higher than that

in group II. Interestingly, the basal core promoter (BCP) double mutations

(A1762T/G1764A) in group I is significantly higher than those in group II as

well.

Conclusions

In patients with HBV infection, the coexistence of HBsAg and anti-HBs is

associated with an increased aa variability in several key areas of HBV genome.

The molecular characteristic of HBV in HBsAg and anti-HBs positive patients is

distinct and worth further studies.

[Guest guest]

http://www.journalofclinicalvirology.com/article/PIIS1386653211002861/abstract?r\

ss=yes

Journal of Clinical Virology

Article in Press

Mutations in hepatitis B virus DNA from patients with coexisting HBsAg and

anti-HBs

Yu Chen, Fuchu Qian, Quan Yuan, Xuefen Li, Wei Wu, Xichao Guo, Lanjuan Li

Received 25 February 2011; received in revised form 6 May 2011; accepted 8 July

2011. published online 16 August 2011.

Corrected Proof

Abstract

Background

The serological markers with coexistence of hepatitis B surface antigen (HBsAg)

and antibody to HBsAg (anti-HBs) of hepatitis B virus (HBV) infection were rare

pattern. The virological significance, immune response and clinical outcome of

these patients remain largely unknown.

Objectives

This research explores the relationship between this serological profile and HBV

genome variants.

Study design

We studied 35 patients both carrying HBsAg and anti-HBs (group I), and 70

patients with HBsAg positive but anti-HBs negative (group II, served as

control). The HBV genome sequences were obtained by direct sequencing of

polymerase chain reaction (PCR) products.

Results

The amino acid (aa) variation within major hydrophilic region (MHR), especially

in the first loop (aa124-137) of “a” determinant in group I is significantly

higher than those in group II. The aa variation of cytotoxic lymphocyte (CTL)

epitope in HBsAg (aa87–aa95) in group I is also significantly higher than that

in group II. Interestingly, the basal core promoter (BCP) double mutations

(A1762T/G1764A) in group I is significantly higher than those in group II as

well.

Conclusions

In patients with HBV infection, the coexistence of HBsAg and anti-HBs is

associated with an increased aa variability in several key areas of HBV genome.

The molecular characteristic of HBV in HBsAg and anti-HBs positive patients is

distinct and worth further studies.