Virologic characteristics of hepatitis B virus in patients infected via maternal-fetal transmission

[Guest guest]

http://www.wjgnet.com/1007-9327/abstract_en.asp?f=5674 & v=14

World J Gastroenterol 2008 October;14(37):5674-5682

Virologic characteristics of hepatitis B virus in patients infected via

maternal-fetal transmission

Shen T, Yan XM, Zou YL, Gao JM, Dong H.

Institute of Basic Medicine of the First People's Hospital of Yunnan Province,

157 Jinbi Road, Kunming 650032, Yunnan Province, China. yxmin08@...

AIM: To determine whether HBV with the same characteristics causes dissimilar

mutations in different hosts. METHODS: Full-length HBV genome was amplified and

linked with pMD T18 vector. Positive clones were selected by double-restriction

endonuclease digestion (EcoRI and HindIII) and PCR. Twenty seven clones were

randomly selected from an asymptomatic mother [at two time points: 602 (1 d) and

6022 (6 mo)] and her son [602 (S)], and the phylogenetic and mutational analysis

was performed using BioEditor, Clustal X and MEGA software. Potential immune

epitopes were determined by the Stabilized Matrix Method (SMM), SMM-Align Method

and Emini Surface Accessibility Prediction. RESULTS: All of the 27 sequences

were genotype C, the divergence between the mother and son was 0%-0.8%. Compared

with another 50 complete sequences of genotype C, the mother and her son each

had 13 specific nucleotides that differed from the other genotype C isolates. AA

1-11 deletion in preS1 was the dominant mutation in the mother (14/18). The

1762T/1764A double mutation existed in all clones of the mother, 3 of them were

also coupled with G1896A mutation, but none were found in the son. 17 bp

deletion starting at nucleotide 2330 was the major mutation (5/9) in the son,

which caused seven potential HLA class I epitopes and one B cell epitope

deletion, and produced a presumptive new start codon, downstream from the

original one of the P gene. CONCLUSION: The HBV strain in the son came from his

mother, and discrepant mutation occurred in the mother and her son during

infection.

[Guest guest]

http://www.wjgnet.com/1007-9327/abstract_en.asp?f=5674 & v=14

World J Gastroenterol 2008 October;14(37):5674-5682

Virologic characteristics of hepatitis B virus in patients infected via

maternal-fetal transmission

Shen T, Yan XM, Zou YL, Gao JM, Dong H.

Institute of Basic Medicine of the First People's Hospital of Yunnan Province,

157 Jinbi Road, Kunming 650032, Yunnan Province, China. yxmin08@...

AIM: To determine whether HBV with the same characteristics causes dissimilar

mutations in different hosts. METHODS: Full-length HBV genome was amplified and

linked with pMD T18 vector. Positive clones were selected by double-restriction

endonuclease digestion (EcoRI and HindIII) and PCR. Twenty seven clones were

randomly selected from an asymptomatic mother [at two time points: 602 (1 d) and

6022 (6 mo)] and her son [602 (S)], and the phylogenetic and mutational analysis

was performed using BioEditor, Clustal X and MEGA software. Potential immune

epitopes were determined by the Stabilized Matrix Method (SMM), SMM-Align Method

and Emini Surface Accessibility Prediction. RESULTS: All of the 27 sequences

were genotype C, the divergence between the mother and son was 0%-0.8%. Compared

with another 50 complete sequences of genotype C, the mother and her son each

had 13 specific nucleotides that differed from the other genotype C isolates. AA

1-11 deletion in preS1 was the dominant mutation in the mother (14/18). The

1762T/1764A double mutation existed in all clones of the mother, 3 of them were

also coupled with G1896A mutation, but none were found in the son. 17 bp

deletion starting at nucleotide 2330 was the major mutation (5/9) in the son,

which caused seven potential HLA class I epitopes and one B cell epitope

deletion, and produced a presumptive new start codon, downstream from the

original one of the P gene. CONCLUSION: The HBV strain in the son came from his

mother, and discrepant mutation occurred in the mother and her son during

infection.