Circulating microRNAs in hepatitis B virus–infected patients

[Guest guest]

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2893.2011.01443.x/abstract

Circulating microRNAs in hepatitis B virus–infected patients

F. Ji1, B. Yang1, X. Peng2, H. Ding3, H. You4, P. Tien1

Article first published online: 7 MAR 2011

DOI: 10.1111/j.1365-2893.2011.01443.x

© 2011 Blackwell Publishing Ltd

Issue

Journal of Viral Hepatitis

Early View (Articles online in advance of print)

Summary.  MicroRNAs (miRNAs) are stably present in human serum. The

relationship between circulating miRNAs and hepatitis B virus (HBV) infected

liver disease has not been previously reported. Applied Biosystems array-based

miRNA expression profiling was performed on pooled sera obtained from identified

groups of chronic asymptomatic carriers (ASC), patients with chronic hepatitis B

(CHB) and HBV-associated acute-on-chronic liver failure (ACLF), as well as

healthy controls (HC). Nine miRNAs were verified in more clinical samples by

RT-PCR. The correlation between miRNAs expression and the relationship between

miRNA levels and clinical characteristics was analysed. Results showed that

circulating miRNAs were detected in all disease and control samples, and their

numbers increased with symptom severity, from 37 in HC, 77 in ASC, 101 in CHB,

to 135 in ACLF. The expression levels of most miRNAs were also up-regulated in

HBV-infected patients when compared to HC. Expression of the liver-specific

miR-122 was significantly up-regulated in HBV-infected patients. Concomitant

regulation of miRNAs not in clusters was disrupted by HBV infection. However,

such disruption was not observed for miRNAs in paralogous clusters. Furthermore,

the level of miRNAs in the CHB serum was up-regulated most in hepatitis B e

antigen–positive patients. The expression levels of miR-122 and miR-194

correlated negatively with the age of patients with CHB or ACLF. Functional

analysis showed that miR-122 could inhibit HBV replication in Huh7 and HepG2

cells. In all, our study revealed that a number of miRNAs were differentially

expressed during HBV infection and underscored the potential importance of

miR-122 in the infection process.

[Guest guest]

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2893.2011.01443.x/abstract

Circulating microRNAs in hepatitis B virus–infected patients

F. Ji1, B. Yang1, X. Peng2, H. Ding3, H. You4, P. Tien1

Article first published online: 7 MAR 2011

DOI: 10.1111/j.1365-2893.2011.01443.x

© 2011 Blackwell Publishing Ltd

Issue

Journal of Viral Hepatitis

Early View (Articles online in advance of print)

Summary.  MicroRNAs (miRNAs) are stably present in human serum. The

relationship between circulating miRNAs and hepatitis B virus (HBV) infected

liver disease has not been previously reported. Applied Biosystems array-based

miRNA expression profiling was performed on pooled sera obtained from identified

groups of chronic asymptomatic carriers (ASC), patients with chronic hepatitis B

(CHB) and HBV-associated acute-on-chronic liver failure (ACLF), as well as

healthy controls (HC). Nine miRNAs were verified in more clinical samples by

RT-PCR. The correlation between miRNAs expression and the relationship between

miRNA levels and clinical characteristics was analysed. Results showed that

circulating miRNAs were detected in all disease and control samples, and their

numbers increased with symptom severity, from 37 in HC, 77 in ASC, 101 in CHB,

to 135 in ACLF. The expression levels of most miRNAs were also up-regulated in

HBV-infected patients when compared to HC. Expression of the liver-specific

miR-122 was significantly up-regulated in HBV-infected patients. Concomitant

regulation of miRNAs not in clusters was disrupted by HBV infection. However,

such disruption was not observed for miRNAs in paralogous clusters. Furthermore,

the level of miRNAs in the CHB serum was up-regulated most in hepatitis B e

antigen–positive patients. The expression levels of miR-122 and miR-194

correlated negatively with the age of patients with CHB or ACLF. Functional

analysis showed that miR-122 could inhibit HBV replication in Huh7 and HepG2

cells. In all, our study revealed that a number of miRNAs were differentially

expressed during HBV infection and underscored the potential importance of

miR-122 in the infection process.