Studies on tissue expression of HBV in children with chronic hepatitis type B using Immunomax technique.

[Guest guest]

Pol J Pathol 1999;50(4):249-58

Studies on tissue expression of HBV in children with chronic hepatitis type

B using Immunomax technique.

Kasprzak A, Biczysko W, Zabel M, Wysocki J, Surdyk-Zasada J

Department of Histology and Embryology, K. Marcinkowski University of

Medical Sciences, Poznan.

The study aimed at employing the Immunomax technique to detect the markers

of HBV replication (HBcAg and HBV-DNA) in liver biopsy material, obtained

from children with chronic hepatitis type B. In line with the currently

modified classification of chronic hepatitis and with the increasing

potential of antiviral therapy it seemed purposeful to supplement routine

staining techniques with studies at the molecular level. Our studies

demonstrated the effective detection of both the core antigen and HBV-DNA in

liver tissue in children using immunocytochemical techniques and in situ

hybridization, amplified with the Immunomax technique. HBcAg was detected in

26 out of 27 liver biopsies from patients with chronic hepatitis type B and

with replication of the virus. HBV-DNA was detected in all study children

with HBV infection and in 2 out of 5 cases of chronic hepatitis of a

distinct etiology. No significant relationships could be found between the

detection of tissue HBV markers on the one hand and the intensity of

inflammatory lesions or severity of fibrosis on the other.

PMID: 10721265, UI: 20186043

------------------------

[Guest guest]

Pol J Pathol 1999;50(4):249-58

Studies on tissue expression of HBV in children with chronic hepatitis type

B using Immunomax technique.

Kasprzak A, Biczysko W, Zabel M, Wysocki J, Surdyk-Zasada J

Department of Histology and Embryology, K. Marcinkowski University of

Medical Sciences, Poznan.

The study aimed at employing the Immunomax technique to detect the markers

of HBV replication (HBcAg and HBV-DNA) in liver biopsy material, obtained

from children with chronic hepatitis type B. In line with the currently

modified classification of chronic hepatitis and with the increasing

potential of antiviral therapy it seemed purposeful to supplement routine

staining techniques with studies at the molecular level. Our studies

demonstrated the effective detection of both the core antigen and HBV-DNA in

liver tissue in children using immunocytochemical techniques and in situ

hybridization, amplified with the Immunomax technique. HBcAg was detected in

26 out of 27 liver biopsies from patients with chronic hepatitis type B and

with replication of the virus. HBV-DNA was detected in all study children

with HBV infection and in 2 out of 5 cases of chronic hepatitis of a

distinct etiology. No significant relationships could be found between the

detection of tissue HBV markers on the one hand and the intensity of

inflammatory lesions or severity of fibrosis on the other.

PMID: 10721265, UI: 20186043

------------------------