Correlation between serum hepatitis B virus core-related antigen and intrahepatic covalently closed circular DNA in chronic hepatitis B patients

[Guest guest]

J Med Virol. 2009 Jan;81(1):27-33.

Correlation between serum hepatitis B virus core-related antigen and

intrahepatic covalently closed circular DNA in chronic hepatitis B patients.

Suzuki F, Miyakoshi H, Kobayashi M, Kumada H.

Department of Hepatology, Toranomon Hospital, Tokyo, Japan.

Nucleos(t)ide analogues are utilized for the treatment of chronic HBV infection,

and HBe seroconversion and HBV DNA levels are commonly used as markers of viral

status and as primary treatment endpoints. Recently, a new assay was prepared

for the detection of serum HBV core-related antigen (HBcrAg), consisting of

HBcAg, HBeAg, and p22cr, which is a precore protein from amino acid -28 to at

least amino acid 150, by coding the precore/core region. In this study, we

examined the correlation between serum HBcrAg concentration and viral status by

the analysis of serum HBeAg, HBsAg, peripheral HBV DNA, and intrahepatic

covalently closed circular DNA (cccDNA) in 57 chronic hepatitis B patients.

Intrahepatic cccDNA was detected in all 57 patients, 42 patients were

HBcrAg-positive, and serum HBcrAg concentration level was closely correlated

with cccDNA. Additionally, positive HBcrAg concentration level results were

observed in 6 out of 13 HBsAg seroclearance patients and 20 out of 31 HBV

DNA-negative patients. Moreover, the correlation between HBcrAg and cccDNA in

these 31 HBV DNA-negative patients was statistically significant (r = 0.482, P =

0.006). These data suggest that serum HBcrAg concentration is well correlated

with intrahepatic cccDNA level, and that the measurement of serum HBcrAg may be

clinically useful for monitoring intrahepatic HBV viral status, especially in

patients under treatment with nucleos(t)ide analogues. J. Med. Virol. 81:27-33,

2009. © 2008 Wiley-Liss, Inc.

PMID: 19031469 [PubMed - in process]

[Guest guest]

J Med Virol. 2009 Jan;81(1):27-33.

Correlation between serum hepatitis B virus core-related antigen and

intrahepatic covalently closed circular DNA in chronic hepatitis B patients.

Suzuki F, Miyakoshi H, Kobayashi M, Kumada H.

Department of Hepatology, Toranomon Hospital, Tokyo, Japan.

Nucleos(t)ide analogues are utilized for the treatment of chronic HBV infection,

and HBe seroconversion and HBV DNA levels are commonly used as markers of viral

status and as primary treatment endpoints. Recently, a new assay was prepared

for the detection of serum HBV core-related antigen (HBcrAg), consisting of

HBcAg, HBeAg, and p22cr, which is a precore protein from amino acid -28 to at

least amino acid 150, by coding the precore/core region. In this study, we

examined the correlation between serum HBcrAg concentration and viral status by

the analysis of serum HBeAg, HBsAg, peripheral HBV DNA, and intrahepatic

covalently closed circular DNA (cccDNA) in 57 chronic hepatitis B patients.

Intrahepatic cccDNA was detected in all 57 patients, 42 patients were

HBcrAg-positive, and serum HBcrAg concentration level was closely correlated

with cccDNA. Additionally, positive HBcrAg concentration level results were

observed in 6 out of 13 HBsAg seroclearance patients and 20 out of 31 HBV

DNA-negative patients. Moreover, the correlation between HBcrAg and cccDNA in

these 31 HBV DNA-negative patients was statistically significant (r = 0.482, P =

0.006). These data suggest that serum HBcrAg concentration is well correlated

with intrahepatic cccDNA level, and that the measurement of serum HBcrAg may be

clinically useful for monitoring intrahepatic HBV viral status, especially in

patients under treatment with nucleos(t)ide analogues. J. Med. Virol. 81:27-33,

2009. © 2008 Wiley-Liss, Inc.

PMID: 19031469 [PubMed - in process]

[Guest guest]

J Med Virol. 2009 Jan;81(1):27-33.

Correlation between serum hepatitis B virus core-related antigen and

intrahepatic covalently closed circular DNA in chronic hepatitis B patients.

Suzuki F, Miyakoshi H, Kobayashi M, Kumada H.

Department of Hepatology, Toranomon Hospital, Tokyo, Japan.

Nucleos(t)ide analogues are utilized for the treatment of chronic HBV infection,

and HBe seroconversion and HBV DNA levels are commonly used as markers of viral

status and as primary treatment endpoints. Recently, a new assay was prepared

for the detection of serum HBV core-related antigen (HBcrAg), consisting of

HBcAg, HBeAg, and p22cr, which is a precore protein from amino acid -28 to at

least amino acid 150, by coding the precore/core region. In this study, we

examined the correlation between serum HBcrAg concentration and viral status by

the analysis of serum HBeAg, HBsAg, peripheral HBV DNA, and intrahepatic

covalently closed circular DNA (cccDNA) in 57 chronic hepatitis B patients.

Intrahepatic cccDNA was detected in all 57 patients, 42 patients were

HBcrAg-positive, and serum HBcrAg concentration level was closely correlated

with cccDNA. Additionally, positive HBcrAg concentration level results were

observed in 6 out of 13 HBsAg seroclearance patients and 20 out of 31 HBV

DNA-negative patients. Moreover, the correlation between HBcrAg and cccDNA in

these 31 HBV DNA-negative patients was statistically significant (r = 0.482, P =

0.006). These data suggest that serum HBcrAg concentration is well correlated

with intrahepatic cccDNA level, and that the measurement of serum HBcrAg may be

clinically useful for monitoring intrahepatic HBV viral status, especially in

patients under treatment with nucleos(t)ide analogues. J. Med. Virol. 81:27-33,

2009. © 2008 Wiley-Liss, Inc.

PMID: 19031469 [PubMed - in process]

[Guest guest]

J Med Virol. 2009 Jan;81(1):27-33.

Correlation between serum hepatitis B virus core-related antigen and

intrahepatic covalently closed circular DNA in chronic hepatitis B patients.

Suzuki F, Miyakoshi H, Kobayashi M, Kumada H.

Department of Hepatology, Toranomon Hospital, Tokyo, Japan.

Nucleos(t)ide analogues are utilized for the treatment of chronic HBV infection,

and HBe seroconversion and HBV DNA levels are commonly used as markers of viral

status and as primary treatment endpoints. Recently, a new assay was prepared

for the detection of serum HBV core-related antigen (HBcrAg), consisting of

HBcAg, HBeAg, and p22cr, which is a precore protein from amino acid -28 to at

least amino acid 150, by coding the precore/core region. In this study, we

examined the correlation between serum HBcrAg concentration and viral status by

the analysis of serum HBeAg, HBsAg, peripheral HBV DNA, and intrahepatic

covalently closed circular DNA (cccDNA) in 57 chronic hepatitis B patients.

Intrahepatic cccDNA was detected in all 57 patients, 42 patients were

HBcrAg-positive, and serum HBcrAg concentration level was closely correlated

with cccDNA. Additionally, positive HBcrAg concentration level results were

observed in 6 out of 13 HBsAg seroclearance patients and 20 out of 31 HBV

DNA-negative patients. Moreover, the correlation between HBcrAg and cccDNA in

these 31 HBV DNA-negative patients was statistically significant (r = 0.482, P =

0.006). These data suggest that serum HBcrAg concentration is well correlated

with intrahepatic cccDNA level, and that the measurement of serum HBcrAg may be

clinically useful for monitoring intrahepatic HBV viral status, especially in

patients under treatment with nucleos(t)ide analogues. J. Med. Virol. 81:27-33,

2009. © 2008 Wiley-Liss, Inc.

PMID: 19031469 [PubMed - in process]