Genotypic Distribution of Hepatitis B Virus (HBV) Among Acute Cases of HBV Infection, Selected United States Counties, 1999–2005

[Guest guest]

http://cid.oxfordjournals.org/content/53/8/751.abstract?etoc

Genotypic Distribution of Hepatitis B Virus (HBV) Among Acute Cases of HBV

Infection, Selected United States Counties, 1999–2005

Eyasu H. Teshalea, Sumathi Ramachandrana, Guo-liang Xia, Henry , a

Groeger, Vaughn Barry, Dale J. Hu, D. Holmberg, Deborah Holtzman, W.

Ward, Chong-Gee Teoa, and Yuri Khudyakov

+

Author Affiliations

Division of Viral Hepatitis, National Center for HIV, Hepatitis, TB and STD

Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia

Correspondence: Eyasu Teshale, MD, Division of Viral Hepatitis, National Center

for HIV, Hepatitis, TB and STD Prevention, Centers for Disease Control and

Prevention, 1600 Clifton Rd, Atlanta, GA 30333 (eht4@...).

Abstract

Background. Knowledge of the genotypic distribution of hepatitis B virus (HBV)

facilitates epidemiologic tracking and surveillance of HBV infection as well as

prediction of its disease burden. In the United States, HBV genotyping studies

have been conducted for chronic but not acute hepatitis B.

Methods. Serum samples were collected from patients with acute hepatitis B

cases reported from the 6 counties that participated in the Sentinel Counties

Study of Acute Viral Hepatitis from 1999 through 2005. Polymerase chain reaction

followed by nucleotide sequencing of a 435–base pair segment of the HBV S gene

was performed, and the sequences were phylogenetically analyzed.

Results. Of 614 patients identified with available serum samples, 75% were

infected with genotype A HBV and 18% were infected with genotype D HBV.

Thirty-two percent of genotype A sequences constituted a single subgenotype A2

cluster. The odds of infection with genotype A (vs with genotype D) were 5 times

greater among black individuals than among Hispanic individuals (odds ratio

[OR], 5; 95% confidence interval [CI], 2.3–10.7). The odds of infection with

genotype A were 49, 8, and 4 times greater among patients from Jefferson County

(Alabama), Pinellas County (Florida), and San Francisco (California),

respectively, than among those living in Denver County (Colorado). Genotype A

was less common among recent injection drug users than it was among

non–injection drug users (OR, 0.2; 95% CI, 0.1–0.4).

Conclusions. HBV genotype distribution was significantly associated with

ethnicity, place of residence, and risk behavior.

Received March 9, 2011.

Accepted June 28, 2011.

Published by Oxford University Press on behalf of the Infectious Diseases

Society of America.

[Guest guest]

http://cid.oxfordjournals.org/content/53/8/751.abstract?etoc

Genotypic Distribution of Hepatitis B Virus (HBV) Among Acute Cases of HBV

Infection, Selected United States Counties, 1999–2005

Eyasu H. Teshalea, Sumathi Ramachandrana, Guo-liang Xia, Henry , a

Groeger, Vaughn Barry, Dale J. Hu, D. Holmberg, Deborah Holtzman, W.

Ward, Chong-Gee Teoa, and Yuri Khudyakov

+

Author Affiliations

Division of Viral Hepatitis, National Center for HIV, Hepatitis, TB and STD

Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia

Correspondence: Eyasu Teshale, MD, Division of Viral Hepatitis, National Center

for HIV, Hepatitis, TB and STD Prevention, Centers for Disease Control and

Prevention, 1600 Clifton Rd, Atlanta, GA 30333 (eht4@...).

Abstract

Background. Knowledge of the genotypic distribution of hepatitis B virus (HBV)

facilitates epidemiologic tracking and surveillance of HBV infection as well as

prediction of its disease burden. In the United States, HBV genotyping studies

have been conducted for chronic but not acute hepatitis B.

Methods. Serum samples were collected from patients with acute hepatitis B

cases reported from the 6 counties that participated in the Sentinel Counties

Study of Acute Viral Hepatitis from 1999 through 2005. Polymerase chain reaction

followed by nucleotide sequencing of a 435–base pair segment of the HBV S gene

was performed, and the sequences were phylogenetically analyzed.

Results. Of 614 patients identified with available serum samples, 75% were

infected with genotype A HBV and 18% were infected with genotype D HBV.

Thirty-two percent of genotype A sequences constituted a single subgenotype A2

cluster. The odds of infection with genotype A (vs with genotype D) were 5 times

greater among black individuals than among Hispanic individuals (odds ratio

[OR], 5; 95% confidence interval [CI], 2.3–10.7). The odds of infection with

genotype A were 49, 8, and 4 times greater among patients from Jefferson County

(Alabama), Pinellas County (Florida), and San Francisco (California),

respectively, than among those living in Denver County (Colorado). Genotype A

was less common among recent injection drug users than it was among

non–injection drug users (OR, 0.2; 95% CI, 0.1–0.4).

Conclusions. HBV genotype distribution was significantly associated with

ethnicity, place of residence, and risk behavior.

Received March 9, 2011.

Accepted June 28, 2011.

Published by Oxford University Press on behalf of the Infectious Diseases

Society of America.

[Guest guest]

http://cid.oxfordjournals.org/content/53/8/751.abstract?etoc

Genotypic Distribution of Hepatitis B Virus (HBV) Among Acute Cases of HBV

Infection, Selected United States Counties, 1999–2005

Eyasu H. Teshalea, Sumathi Ramachandrana, Guo-liang Xia, Henry , a

Groeger, Vaughn Barry, Dale J. Hu, D. Holmberg, Deborah Holtzman, W.

Ward, Chong-Gee Teoa, and Yuri Khudyakov

+

Author Affiliations

Division of Viral Hepatitis, National Center for HIV, Hepatitis, TB and STD

Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia

Correspondence: Eyasu Teshale, MD, Division of Viral Hepatitis, National Center

for HIV, Hepatitis, TB and STD Prevention, Centers for Disease Control and

Prevention, 1600 Clifton Rd, Atlanta, GA 30333 (eht4@...).

Abstract

Background. Knowledge of the genotypic distribution of hepatitis B virus (HBV)

facilitates epidemiologic tracking and surveillance of HBV infection as well as

prediction of its disease burden. In the United States, HBV genotyping studies

have been conducted for chronic but not acute hepatitis B.

Methods. Serum samples were collected from patients with acute hepatitis B

cases reported from the 6 counties that participated in the Sentinel Counties

Study of Acute Viral Hepatitis from 1999 through 2005. Polymerase chain reaction

followed by nucleotide sequencing of a 435–base pair segment of the HBV S gene

was performed, and the sequences were phylogenetically analyzed.

Results. Of 614 patients identified with available serum samples, 75% were

infected with genotype A HBV and 18% were infected with genotype D HBV.

Thirty-two percent of genotype A sequences constituted a single subgenotype A2

cluster. The odds of infection with genotype A (vs with genotype D) were 5 times

greater among black individuals than among Hispanic individuals (odds ratio

[OR], 5; 95% confidence interval [CI], 2.3–10.7). The odds of infection with

genotype A were 49, 8, and 4 times greater among patients from Jefferson County

(Alabama), Pinellas County (Florida), and San Francisco (California),

respectively, than among those living in Denver County (Colorado). Genotype A

was less common among recent injection drug users than it was among

non–injection drug users (OR, 0.2; 95% CI, 0.1–0.4).

Conclusions. HBV genotype distribution was significantly associated with

ethnicity, place of residence, and risk behavior.

Received March 9, 2011.

Accepted June 28, 2011.

Published by Oxford University Press on behalf of the Infectious Diseases

Society of America.

[Guest guest]

http://cid.oxfordjournals.org/content/53/8/751.abstract?etoc

Genotypic Distribution of Hepatitis B Virus (HBV) Among Acute Cases of HBV

Infection, Selected United States Counties, 1999–2005

Eyasu H. Teshalea, Sumathi Ramachandrana, Guo-liang Xia, Henry , a

Groeger, Vaughn Barry, Dale J. Hu, D. Holmberg, Deborah Holtzman, W.

Ward, Chong-Gee Teoa, and Yuri Khudyakov

+

Author Affiliations

Division of Viral Hepatitis, National Center for HIV, Hepatitis, TB and STD

Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia

Correspondence: Eyasu Teshale, MD, Division of Viral Hepatitis, National Center

for HIV, Hepatitis, TB and STD Prevention, Centers for Disease Control and

Prevention, 1600 Clifton Rd, Atlanta, GA 30333 (eht4@...).

Abstract

Background. Knowledge of the genotypic distribution of hepatitis B virus (HBV)

facilitates epidemiologic tracking and surveillance of HBV infection as well as

prediction of its disease burden. In the United States, HBV genotyping studies

have been conducted for chronic but not acute hepatitis B.

Methods. Serum samples were collected from patients with acute hepatitis B

cases reported from the 6 counties that participated in the Sentinel Counties

Study of Acute Viral Hepatitis from 1999 through 2005. Polymerase chain reaction

followed by nucleotide sequencing of a 435–base pair segment of the HBV S gene

was performed, and the sequences were phylogenetically analyzed.

Results. Of 614 patients identified with available serum samples, 75% were

infected with genotype A HBV and 18% were infected with genotype D HBV.

Thirty-two percent of genotype A sequences constituted a single subgenotype A2

cluster. The odds of infection with genotype A (vs with genotype D) were 5 times

greater among black individuals than among Hispanic individuals (odds ratio

[OR], 5; 95% confidence interval [CI], 2.3–10.7). The odds of infection with

genotype A were 49, 8, and 4 times greater among patients from Jefferson County

(Alabama), Pinellas County (Florida), and San Francisco (California),

respectively, than among those living in Denver County (Colorado). Genotype A

was less common among recent injection drug users than it was among

non–injection drug users (OR, 0.2; 95% CI, 0.1–0.4).

Conclusions. HBV genotype distribution was significantly associated with

ethnicity, place of residence, and risk behavior.

Received March 9, 2011.

Accepted June 28, 2011.

Published by Oxford University Press on behalf of the Infectious Diseases

Society of America.