Natural killer cells suppress full cycle HCV infection of human hepatocytes

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Natural killer cells suppress full cycle HCV infection of human hepatocytes

Authors: Wang, S.-H.; Huang, C.-X.1; Ye, L.2; Wang, X.2; Song, L.2; Wang,

Y.-J.2; Liang, H.2; Huang, X.-Y.1; Ho, W.-Z.2

Source: Journal of Viral Hepatitis, Volume 15, Number 12, December 2008 , pp.

855-864(10)

Publisher: Blackwell Publishing

Abstract:

Summary. 

The role of natural killer (NK) cells in controlling hepatitis C virus (HCV)

infection and replication has not been fully delineated. We examined NK

cell-mediated noncytolytic effect on full cycle HCV infection of human

hepatocytes. Human hepatocytes (Huh7.5.1 cells) co-cultured with NK cells or

treated with supernatants (SN) from NK cells cultures had significantly lower

levels of HCV RNA and protein than control cells. This NK cell-mediated anti-HCV

activity could be largely abolished by antibody to interferon-gamma (IFN-γ).

The investigation of the mechanisms for NK cell-mediated anti-HCV activity

showed that NK SN-treated hepatocytes expressed higher levels of IFN-α/β than

the control cells. NK SN also enhanced IFN regulatory factor-3 and 7 expression

in the hepatocytes. In addition, NK SN enhanced the expression of signal

transducer and activator of transcription 1 and 2, the nuclear factors that are

essential for the activation of IFN-mediated antiviral pathways. These data

provide direct evidence at cellular and molecular levels that NK cells have a

key role in suppressing HCV infection of and replication in human hepatocytes.

Document Type: Research article

DOI: 10.1111/j.1365-2893.2008.01014.x

Affiliations: 1: Renmin Hospital of Wuhan University, Wuhan University, Hubei,

China 2: Division of Allergy and Immunology, ph Stokes, Jr. Research

Institute at The Children's Hospital of Philadelphia, Department of Pediatrics,

University of Pennsylvania School of Medicine, Philadelphia, PA, USA

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Natural killer cells suppress full cycle HCV infection of human hepatocytes

Authors: Wang, S.-H.; Huang, C.-X.1; Ye, L.2; Wang, X.2; Song, L.2; Wang,

Y.-J.2; Liang, H.2; Huang, X.-Y.1; Ho, W.-Z.2

Source: Journal of Viral Hepatitis, Volume 15, Number 12, December 2008 , pp.

855-864(10)

Publisher: Blackwell Publishing

Abstract:

Summary. 

The role of natural killer (NK) cells in controlling hepatitis C virus (HCV)

infection and replication has not been fully delineated. We examined NK

cell-mediated noncytolytic effect on full cycle HCV infection of human

hepatocytes. Human hepatocytes (Huh7.5.1 cells) co-cultured with NK cells or

treated with supernatants (SN) from NK cells cultures had significantly lower

levels of HCV RNA and protein than control cells. This NK cell-mediated anti-HCV

activity could be largely abolished by antibody to interferon-gamma (IFN-γ).

The investigation of the mechanisms for NK cell-mediated anti-HCV activity

showed that NK SN-treated hepatocytes expressed higher levels of IFN-α/β than

the control cells. NK SN also enhanced IFN regulatory factor-3 and 7 expression

in the hepatocytes. In addition, NK SN enhanced the expression of signal

transducer and activator of transcription 1 and 2, the nuclear factors that are

essential for the activation of IFN-mediated antiviral pathways. These data

provide direct evidence at cellular and molecular levels that NK cells have a

key role in suppressing HCV infection of and replication in human hepatocytes.

Document Type: Research article

DOI: 10.1111/j.1365-2893.2008.01014.x

Affiliations: 1: Renmin Hospital of Wuhan University, Wuhan University, Hubei,

China 2: Division of Allergy and Immunology, ph Stokes, Jr. Research

Institute at The Children's Hospital of Philadelphia, Department of Pediatrics,

University of Pennsylvania School of Medicine, Philadelphia, PA, USA

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essionid=berbitciulg8e.alice

Natural killer cells suppress full cycle HCV infection of human hepatocytes

Authors: Wang, S.-H.; Huang, C.-X.1; Ye, L.2; Wang, X.2; Song, L.2; Wang,

Y.-J.2; Liang, H.2; Huang, X.-Y.1; Ho, W.-Z.2

Source: Journal of Viral Hepatitis, Volume 15, Number 12, December 2008 , pp.

855-864(10)

Publisher: Blackwell Publishing

Abstract:

Summary. 

The role of natural killer (NK) cells in controlling hepatitis C virus (HCV)

infection and replication has not been fully delineated. We examined NK

cell-mediated noncytolytic effect on full cycle HCV infection of human

hepatocytes. Human hepatocytes (Huh7.5.1 cells) co-cultured with NK cells or

treated with supernatants (SN) from NK cells cultures had significantly lower

levels of HCV RNA and protein than control cells. This NK cell-mediated anti-HCV

activity could be largely abolished by antibody to interferon-gamma (IFN-γ).

The investigation of the mechanisms for NK cell-mediated anti-HCV activity

showed that NK SN-treated hepatocytes expressed higher levels of IFN-α/β than

the control cells. NK SN also enhanced IFN regulatory factor-3 and 7 expression

in the hepatocytes. In addition, NK SN enhanced the expression of signal

transducer and activator of transcription 1 and 2, the nuclear factors that are

essential for the activation of IFN-mediated antiviral pathways. These data

provide direct evidence at cellular and molecular levels that NK cells have a

key role in suppressing HCV infection of and replication in human hepatocytes.

Document Type: Research article

DOI: 10.1111/j.1365-2893.2008.01014.x

Affiliations: 1: Renmin Hospital of Wuhan University, Wuhan University, Hubei,

China 2: Division of Allergy and Immunology, ph Stokes, Jr. Research

Institute at The Children's Hospital of Philadelphia, Department of Pediatrics,

University of Pennsylvania School of Medicine, Philadelphia, PA, USA

[Guest guest]

http://www.ingentaconnect.com/content/bsc/jvh/2008/00000015/00000012/art00002;js\

essionid=berbitciulg8e.alice

Natural killer cells suppress full cycle HCV infection of human hepatocytes

Authors: Wang, S.-H.; Huang, C.-X.1; Ye, L.2; Wang, X.2; Song, L.2; Wang,

Y.-J.2; Liang, H.2; Huang, X.-Y.1; Ho, W.-Z.2

Source: Journal of Viral Hepatitis, Volume 15, Number 12, December 2008 , pp.

855-864(10)

Publisher: Blackwell Publishing

Abstract:

Summary. 

The role of natural killer (NK) cells in controlling hepatitis C virus (HCV)

infection and replication has not been fully delineated. We examined NK

cell-mediated noncytolytic effect on full cycle HCV infection of human

hepatocytes. Human hepatocytes (Huh7.5.1 cells) co-cultured with NK cells or

treated with supernatants (SN) from NK cells cultures had significantly lower

levels of HCV RNA and protein than control cells. This NK cell-mediated anti-HCV

activity could be largely abolished by antibody to interferon-gamma (IFN-γ).

The investigation of the mechanisms for NK cell-mediated anti-HCV activity

showed that NK SN-treated hepatocytes expressed higher levels of IFN-α/β than

the control cells. NK SN also enhanced IFN regulatory factor-3 and 7 expression

in the hepatocytes. In addition, NK SN enhanced the expression of signal

transducer and activator of transcription 1 and 2, the nuclear factors that are

essential for the activation of IFN-mediated antiviral pathways. These data

provide direct evidence at cellular and molecular levels that NK cells have a

key role in suppressing HCV infection of and replication in human hepatocytes.

Document Type: Research article

DOI: 10.1111/j.1365-2893.2008.01014.x

Affiliations: 1: Renmin Hospital of Wuhan University, Wuhan University, Hubei,

China 2: Division of Allergy and Immunology, ph Stokes, Jr. Research

Institute at The Children's Hospital of Philadelphia, Department of Pediatrics,

University of Pennsylvania School of Medicine, Philadelphia, PA, USA