Antibiotics disrupt gut ecology, metabolism

[Guest guest]

from Binstock

http://www.sciencedaily.com/releases/2011/04/110419214734.htm

This study used a single high dose of streptomycin orally in one arm,

and low doses of several antibiotics in drinking water in another

arm. The results are quite dramatic:

" The most profoundly altered pathways involved steroid

hormones, eicosanoid hormones, sugar, fatty acid, and bile

acid "

Antibiotics Disrupt Gut Ecology,

MetabolismScienceDaily (Apr. 19, 2011) — Humans

carry several pounds of microbes in our gastro-intestinal tracts. Recent

research suggests that this microbial ecosystem plays a variety of

critical roles in our health. Now, working in a mouse model, researchers

from Canada describe many of the interactions between the intestinal

microbiota and host, and show that antibiotics profoundly disrupt

intestinal homeostasis. The research is published in the April 2011 issue

of the journal Antimicrobial Agents and Chemotherapy.

" Intestinal microbes help us digest our food, provide us with

vitamins that we cannot make on our own, and protect us from microbes

that make us sick, amongst other things, " says L Caetano M. Antunes

of the University of British Columbia, a researcher on the study. In this

study, the investigators used powerful mass spectrometry techniques to

detect, identify, and quantify more than two thousand molecules which

they extracted from mouse feces. They then administered antibiotics to

the mice, to kill off most of their gut microbiota, and analyzed the

feces anew.

The second round of mass spectroscopy revealed a very different metabolic

landscape. The levels of 87 percent of the molecules detected had been

shifted up or down by factors ranging from 2-fold to

10,000-fold.

The most profoundly altered pathways involved steroid hormones,

eicosanoid hormones, sugar, fatty acid, and bile acid. " These

hormones have very important functions in our health, " says Antunes.

" They control our immune system, reproductive functions, mineral

balance, sugar metabolism, and many other important aspects of human

metabolism. "

The findings have two important implications, says Antunes. " First,

our work shows that the unnecessary use of antibiotics has deleterious

effects on human health that were previously unappreciated. Also, the

fact that our gut microbes control these important molecules raises the

possibility that manipulating these microbes could be used to modulate

diseases that have hormonal or metabolic origins (such as

inmmunodeficiency, depression, diabetes and others). However, further

studies will be required to understand exactly how our microbial partners

function to modulate human physiology, and to devise ways of using this

information to improve human health. "

(L.C.M. Antunes, J. Han, R.B.R. Ferreira, P. Lolic, C.H. Borchers, and

B.B. Finlay, 2011. Effect of antibiotic treatment on the intestinal

metabolome. Antim. Agents Chemother. 55:1494-1503.)

Email or share this story:

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More

Story Source:

The above story is reprinted (with editorial adaptations by

ScienceDaily staff) from materials provided by

American Society for Microbiology,

via EurekAlert!, a service of

AAAS.

Journal Reference:

L. C. M. Antunes, J. Han, R. B. R. Ferreira, P. Lolic, C. H.

Borchers, B. B. Finlay. Effect of Antibiotic Treatment on the

Intestinal Metabolome. Antimicrobial Agents and Chemotherapy,

2011; 55 (4): 1494 DOI:

10.1128/AAC.01664-10

===========

Antimicrobial Agents and Chemotherapy, April 2011, p. 1494-1503, Vol. 55,

No. 4

0066-4804/11/$12.00+0

doi:10.1128/AAC.01664-10

Copyright © 2011,

American Society for Microbiology. All

Rights Reserved.

Effect of Antibiotic Treatment on the

Intestinal

Metabolome

L. Caetano M. Antunes,1

Jun Han,2 na B. R. Ferreira,1 Petra

Loli

,1,3 Christoph H. Borchers,2, and B. Brett

Finlay1,3*

Laboratories, The University of British Columbia,

Vancouver, British Columbia V6T 1Z4, Canada,1 University of

Genome BC Proteomics Centre, University of , ,

British Columbia V8Z 7X8, Canada,2 Department of Microbiology

and Immunology, The University of British Columbia, Vancouver, British

Columbia V6T 1Z3, Canada3

Received 1 December 2010/ Returned for modification 8 January 2011/

Accepted 21 January 2011

The importance of the mammalian intestinal microbiota to human

health has been intensely studied over the past few years. It

is now clear that the interactions between human hosts and

their associated microbial communities need to be

characterized in molecular detail if we are to truly

understand human physiology. Additionally, the study of such

host-microbe interactions is likely to provide us with new

strategies to manipulate these complex systems to maintain or

restore homeostasis in order to prevent or cure pathological

states. Here, we describe the use of high-throughput

metabolomics to shed light on the interactions between the

intestinal microbiota and the host. We show that antibiotic

treatment disrupts intestinal homeostasis and has a profound

impact on the intestinal metabolome, affecting the levels of

over 87% of all metabolites detected. Many metabolic pathways

that are critical for host physiology were affected, including

bile acid, eicosanoid, and steroid hormone synthesis.

Dissecting the molecular mechanisms involved in the impact of

beneficial microbes on some of these pathways will be

instrumental in understanding the interplay between the host

and its complex resident microbiota and may aid in the design

of new therapeutic strategies that target these

interactions.

Sheri Nakken, former R.N., MA, Hahnemannian

Homeopath

Vaccination Information & Choice Network, Washington State, USA

Vaccines -

http://vaccinationdangers.wordpress.com/ Homeopathy

http://homeopathycures.wordpress.com

Vaccine Dangers, Childhood Disease Classes & Homeopathy

Online/email courses - next classes start April 22

[Guest guest]

from Binstock

http://www.sciencedaily.com/releases/2011/04/110419214734.htm

This study used a single high dose of streptomycin orally in one arm,

and low doses of several antibiotics in drinking water in another

arm. The results are quite dramatic:

" The most profoundly altered pathways involved steroid

hormones, eicosanoid hormones, sugar, fatty acid, and bile

acid "

Antibiotics Disrupt Gut Ecology,

MetabolismScienceDaily (Apr. 19, 2011) — Humans

carry several pounds of microbes in our gastro-intestinal tracts. Recent

research suggests that this microbial ecosystem plays a variety of

critical roles in our health. Now, working in a mouse model, researchers

from Canada describe many of the interactions between the intestinal

microbiota and host, and show that antibiotics profoundly disrupt

intestinal homeostasis. The research is published in the April 2011 issue

of the journal Antimicrobial Agents and Chemotherapy.

" Intestinal microbes help us digest our food, provide us with

vitamins that we cannot make on our own, and protect us from microbes

that make us sick, amongst other things, " says L Caetano M. Antunes

of the University of British Columbia, a researcher on the study. In this

study, the investigators used powerful mass spectrometry techniques to

detect, identify, and quantify more than two thousand molecules which

they extracted from mouse feces. They then administered antibiotics to

the mice, to kill off most of their gut microbiota, and analyzed the

feces anew.

The second round of mass spectroscopy revealed a very different metabolic

landscape. The levels of 87 percent of the molecules detected had been

shifted up or down by factors ranging from 2-fold to

10,000-fold.

The most profoundly altered pathways involved steroid hormones,

eicosanoid hormones, sugar, fatty acid, and bile acid. " These

hormones have very important functions in our health, " says Antunes.

" They control our immune system, reproductive functions, mineral

balance, sugar metabolism, and many other important aspects of human

metabolism. "

The findings have two important implications, says Antunes. " First,

our work shows that the unnecessary use of antibiotics has deleterious

effects on human health that were previously unappreciated. Also, the

fact that our gut microbes control these important molecules raises the

possibility that manipulating these microbes could be used to modulate

diseases that have hormonal or metabolic origins (such as

inmmunodeficiency, depression, diabetes and others). However, further

studies will be required to understand exactly how our microbial partners

function to modulate human physiology, and to devise ways of using this

information to improve human health. "

(L.C.M. Antunes, J. Han, R.B.R. Ferreira, P. Lolic, C.H. Borchers, and

B.B. Finlay, 2011. Effect of antibiotic treatment on the intestinal

metabolome. Antim. Agents Chemother. 55:1494-1503.)

Email or share this story:

|

More

Story Source:

The above story is reprinted (with editorial adaptations by

ScienceDaily staff) from materials provided by

American Society for Microbiology,

via EurekAlert!, a service of

AAAS.

Journal Reference:

L. C. M. Antunes, J. Han, R. B. R. Ferreira, P. Lolic, C. H.

Borchers, B. B. Finlay. Effect of Antibiotic Treatment on the

Intestinal Metabolome. Antimicrobial Agents and Chemotherapy,

2011; 55 (4): 1494 DOI:

10.1128/AAC.01664-10

===========

Antimicrobial Agents and Chemotherapy, April 2011, p. 1494-1503, Vol. 55,

No. 4

0066-4804/11/$12.00+0

doi:10.1128/AAC.01664-10

Copyright © 2011,

American Society for Microbiology. All

Rights Reserved.

Effect of Antibiotic Treatment on the

Intestinal

Metabolome

L. Caetano M. Antunes,1

Jun Han,2 na B. R. Ferreira,1 Petra

Loli

,1,3 Christoph H. Borchers,2, and B. Brett

Finlay1,3*

Laboratories, The University of British Columbia,

Vancouver, British Columbia V6T 1Z4, Canada,1 University of

Genome BC Proteomics Centre, University of , ,

British Columbia V8Z 7X8, Canada,2 Department of Microbiology

and Immunology, The University of British Columbia, Vancouver, British

Columbia V6T 1Z3, Canada3

Received 1 December 2010/ Returned for modification 8 January 2011/

Accepted 21 January 2011

The importance of the mammalian intestinal microbiota to human

health has been intensely studied over the past few years. It

is now clear that the interactions between human hosts and

their associated microbial communities need to be

characterized in molecular detail if we are to truly

understand human physiology. Additionally, the study of such

host-microbe interactions is likely to provide us with new

strategies to manipulate these complex systems to maintain or

restore homeostasis in order to prevent or cure pathological

states. Here, we describe the use of high-throughput

metabolomics to shed light on the interactions between the

intestinal microbiota and the host. We show that antibiotic

treatment disrupts intestinal homeostasis and has a profound

impact on the intestinal metabolome, affecting the levels of

over 87% of all metabolites detected. Many metabolic pathways

that are critical for host physiology were affected, including

bile acid, eicosanoid, and steroid hormone synthesis.

Dissecting the molecular mechanisms involved in the impact of

beneficial microbes on some of these pathways will be

instrumental in understanding the interplay between the host

and its complex resident microbiota and may aid in the design

of new therapeutic strategies that target these

interactions.

Sheri Nakken, former R.N., MA, Hahnemannian

Homeopath

Vaccination Information & Choice Network, Washington State, USA

Vaccines -

http://vaccinationdangers.wordpress.com/ Homeopathy

http://homeopathycures.wordpress.com

Vaccine Dangers, Childhood Disease Classes & Homeopathy

Online/email courses - next classes start April 22

[Guest guest]

from Binstock

http://www.sciencedaily.com/releases/2011/04/110419214734.htm

This study used a single high dose of streptomycin orally in one arm,

and low doses of several antibiotics in drinking water in another

arm. The results are quite dramatic:

" The most profoundly altered pathways involved steroid

hormones, eicosanoid hormones, sugar, fatty acid, and bile

acid "

Antibiotics Disrupt Gut Ecology,

MetabolismScienceDaily (Apr. 19, 2011) — Humans

carry several pounds of microbes in our gastro-intestinal tracts. Recent

research suggests that this microbial ecosystem plays a variety of

critical roles in our health. Now, working in a mouse model, researchers

from Canada describe many of the interactions between the intestinal

microbiota and host, and show that antibiotics profoundly disrupt

intestinal homeostasis. The research is published in the April 2011 issue

of the journal Antimicrobial Agents and Chemotherapy.

" Intestinal microbes help us digest our food, provide us with

vitamins that we cannot make on our own, and protect us from microbes

that make us sick, amongst other things, " says L Caetano M. Antunes

of the University of British Columbia, a researcher on the study. In this

study, the investigators used powerful mass spectrometry techniques to

detect, identify, and quantify more than two thousand molecules which

they extracted from mouse feces. They then administered antibiotics to

the mice, to kill off most of their gut microbiota, and analyzed the

feces anew.

The second round of mass spectroscopy revealed a very different metabolic

landscape. The levels of 87 percent of the molecules detected had been

shifted up or down by factors ranging from 2-fold to

10,000-fold.

The most profoundly altered pathways involved steroid hormones,

eicosanoid hormones, sugar, fatty acid, and bile acid. " These

hormones have very important functions in our health, " says Antunes.

" They control our immune system, reproductive functions, mineral

balance, sugar metabolism, and many other important aspects of human

metabolism. "

The findings have two important implications, says Antunes. " First,

our work shows that the unnecessary use of antibiotics has deleterious

effects on human health that were previously unappreciated. Also, the

fact that our gut microbes control these important molecules raises the

possibility that manipulating these microbes could be used to modulate

diseases that have hormonal or metabolic origins (such as

inmmunodeficiency, depression, diabetes and others). However, further

studies will be required to understand exactly how our microbial partners

function to modulate human physiology, and to devise ways of using this

information to improve human health. "

(L.C.M. Antunes, J. Han, R.B.R. Ferreira, P. Lolic, C.H. Borchers, and

B.B. Finlay, 2011. Effect of antibiotic treatment on the intestinal

metabolome. Antim. Agents Chemother. 55:1494-1503.)

Email or share this story:

|

More

Story Source:

The above story is reprinted (with editorial adaptations by

ScienceDaily staff) from materials provided by

American Society for Microbiology,

via EurekAlert!, a service of

AAAS.

Journal Reference:

L. C. M. Antunes, J. Han, R. B. R. Ferreira, P. Lolic, C. H.

Borchers, B. B. Finlay. Effect of Antibiotic Treatment on the

Intestinal Metabolome. Antimicrobial Agents and Chemotherapy,

2011; 55 (4): 1494 DOI:

10.1128/AAC.01664-10

===========

Antimicrobial Agents and Chemotherapy, April 2011, p. 1494-1503, Vol. 55,

No. 4

0066-4804/11/$12.00+0

doi:10.1128/AAC.01664-10

Copyright © 2011,

American Society for Microbiology. All

Rights Reserved.

Effect of Antibiotic Treatment on the

Intestinal

Metabolome

L. Caetano M. Antunes,1

Jun Han,2 na B. R. Ferreira,1 Petra

Loli

,1,3 Christoph H. Borchers,2, and B. Brett

Finlay1,3*

Laboratories, The University of British Columbia,

Vancouver, British Columbia V6T 1Z4, Canada,1 University of

Genome BC Proteomics Centre, University of , ,

British Columbia V8Z 7X8, Canada,2 Department of Microbiology

and Immunology, The University of British Columbia, Vancouver, British

Columbia V6T 1Z3, Canada3

Received 1 December 2010/ Returned for modification 8 January 2011/

Accepted 21 January 2011

The importance of the mammalian intestinal microbiota to human

health has been intensely studied over the past few years. It

is now clear that the interactions between human hosts and

their associated microbial communities need to be

characterized in molecular detail if we are to truly

understand human physiology. Additionally, the study of such

host-microbe interactions is likely to provide us with new

strategies to manipulate these complex systems to maintain or

restore homeostasis in order to prevent or cure pathological

states. Here, we describe the use of high-throughput

metabolomics to shed light on the interactions between the

intestinal microbiota and the host. We show that antibiotic

treatment disrupts intestinal homeostasis and has a profound

impact on the intestinal metabolome, affecting the levels of

over 87% of all metabolites detected. Many metabolic pathways

that are critical for host physiology were affected, including

bile acid, eicosanoid, and steroid hormone synthesis.

Dissecting the molecular mechanisms involved in the impact of

beneficial microbes on some of these pathways will be

instrumental in understanding the interplay between the host

and its complex resident microbiota and may aid in the design

of new therapeutic strategies that target these

interactions.

Sheri Nakken, former R.N., MA, Hahnemannian

Homeopath

Vaccination Information & Choice Network, Washington State, USA

Vaccines -

http://vaccinationdangers.wordpress.com/ Homeopathy

http://homeopathycures.wordpress.com

Vaccine Dangers, Childhood Disease Classes & Homeopathy

Online/email courses - next classes start April 22

[Guest guest]

from Binstock

http://www.sciencedaily.com/releases/2011/04/110419214734.htm

This study used a single high dose of streptomycin orally in one arm,

and low doses of several antibiotics in drinking water in another

arm. The results are quite dramatic:

" The most profoundly altered pathways involved steroid

hormones, eicosanoid hormones, sugar, fatty acid, and bile

acid "

Antibiotics Disrupt Gut Ecology,

MetabolismScienceDaily (Apr. 19, 2011) — Humans

carry several pounds of microbes in our gastro-intestinal tracts. Recent

research suggests that this microbial ecosystem plays a variety of

critical roles in our health. Now, working in a mouse model, researchers

from Canada describe many of the interactions between the intestinal

microbiota and host, and show that antibiotics profoundly disrupt

intestinal homeostasis. The research is published in the April 2011 issue

of the journal Antimicrobial Agents and Chemotherapy.

" Intestinal microbes help us digest our food, provide us with

vitamins that we cannot make on our own, and protect us from microbes

that make us sick, amongst other things, " says L Caetano M. Antunes

of the University of British Columbia, a researcher on the study. In this

study, the investigators used powerful mass spectrometry techniques to

detect, identify, and quantify more than two thousand molecules which

they extracted from mouse feces. They then administered antibiotics to

the mice, to kill off most of their gut microbiota, and analyzed the

feces anew.

The second round of mass spectroscopy revealed a very different metabolic

landscape. The levels of 87 percent of the molecules detected had been

shifted up or down by factors ranging from 2-fold to

10,000-fold.

The most profoundly altered pathways involved steroid hormones,

eicosanoid hormones, sugar, fatty acid, and bile acid. " These

hormones have very important functions in our health, " says Antunes.

" They control our immune system, reproductive functions, mineral

balance, sugar metabolism, and many other important aspects of human

metabolism. "

The findings have two important implications, says Antunes. " First,

our work shows that the unnecessary use of antibiotics has deleterious

effects on human health that were previously unappreciated. Also, the

fact that our gut microbes control these important molecules raises the

possibility that manipulating these microbes could be used to modulate

diseases that have hormonal or metabolic origins (such as

inmmunodeficiency, depression, diabetes and others). However, further

studies will be required to understand exactly how our microbial partners

function to modulate human physiology, and to devise ways of using this

information to improve human health. "

(L.C.M. Antunes, J. Han, R.B.R. Ferreira, P. Lolic, C.H. Borchers, and

B.B. Finlay, 2011. Effect of antibiotic treatment on the intestinal

metabolome. Antim. Agents Chemother. 55:1494-1503.)

Email or share this story:

|

More

Story Source:

The above story is reprinted (with editorial adaptations by

ScienceDaily staff) from materials provided by

American Society for Microbiology,

via EurekAlert!, a service of

AAAS.

Journal Reference:

L. C. M. Antunes, J. Han, R. B. R. Ferreira, P. Lolic, C. H.

Borchers, B. B. Finlay. Effect of Antibiotic Treatment on the

Intestinal Metabolome. Antimicrobial Agents and Chemotherapy,

2011; 55 (4): 1494 DOI:

10.1128/AAC.01664-10

===========

Antimicrobial Agents and Chemotherapy, April 2011, p. 1494-1503, Vol. 55,

No. 4

0066-4804/11/$12.00+0

doi:10.1128/AAC.01664-10

Copyright © 2011,

American Society for Microbiology. All

Rights Reserved.

Effect of Antibiotic Treatment on the

Intestinal

Metabolome

L. Caetano M. Antunes,1

Jun Han,2 na B. R. Ferreira,1 Petra

Loli

,1,3 Christoph H. Borchers,2, and B. Brett

Finlay1,3*

Laboratories, The University of British Columbia,

Vancouver, British Columbia V6T 1Z4, Canada,1 University of

Genome BC Proteomics Centre, University of , ,

British Columbia V8Z 7X8, Canada,2 Department of Microbiology

and Immunology, The University of British Columbia, Vancouver, British

Columbia V6T 1Z3, Canada3

Received 1 December 2010/ Returned for modification 8 January 2011/

Accepted 21 January 2011

The importance of the mammalian intestinal microbiota to human

health has been intensely studied over the past few years. It

is now clear that the interactions between human hosts and

their associated microbial communities need to be

characterized in molecular detail if we are to truly

understand human physiology. Additionally, the study of such

host-microbe interactions is likely to provide us with new

strategies to manipulate these complex systems to maintain or

restore homeostasis in order to prevent or cure pathological

states. Here, we describe the use of high-throughput

metabolomics to shed light on the interactions between the

intestinal microbiota and the host. We show that antibiotic

treatment disrupts intestinal homeostasis and has a profound

impact on the intestinal metabolome, affecting the levels of

over 87% of all metabolites detected. Many metabolic pathways

that are critical for host physiology were affected, including

bile acid, eicosanoid, and steroid hormone synthesis.

Dissecting the molecular mechanisms involved in the impact of

beneficial microbes on some of these pathways will be

instrumental in understanding the interplay between the host

and its complex resident microbiota and may aid in the design

of new therapeutic strategies that target these

interactions.

Sheri Nakken, former R.N., MA, Hahnemannian

Homeopath

Vaccination Information & Choice Network, Washington State, USA

Vaccines -

http://vaccinationdangers.wordpress.com/ Homeopathy

http://homeopathycures.wordpress.com

Vaccine Dangers, Childhood Disease Classes & Homeopathy

Online/email courses - next classes start April 22