Spindler's Late-Age Mouse Chow Formula for 42% Life Extension

[Guest guest]

Spindler’s late-age mice (60 to 65 human years) on 43.9% CR

had a mean lifespan increase of 42% (1). Below is the

composition of the mouse chow fed to the two groups of mice.

(Thanks Rodney!)

The long-lived CR mice had the same amount of protein and fat

in their diet as the control mice.

The only way the two diets differed was that the calories from

carbohydrates were reduced for the CR mice.

When you pursue your own human CR diet, and consider what

macronutrients (C:P:F carbohydrates, proteins, fats) you

wish to limit to reduce your calories, this information

about the mouse chow formulas for Spindler’s long-lived

late-age cohort mice may possibly influence your thinking.

-- Warren

1. Proc Natl Acad Sci U S A. 2004 Mar 25 [Epub ahead of print]

ph M. Dhahbi, Hyon-Jeen Kim, L. Mote, J. Beaver,

and R. Spindler, " Temporal Linkage Between the Phenotypic

and Genomic Responses to Caloric Restriction " , PMID: 15044709,

[PubMed - as supplied by publisher]

==================================

On 16 Apr 2004, Rodney Nicholson wrote:

Hi folks:

[The CR mouse] diet F05314 is designed in such a way that, in

comparison with mice fed [standard mouse chow] F05312, mice fed 40%

less of F05314 will consume 40% fewer calories but very nearly

identical amounts of all nutrients except carbohydrates.

And the entire reduction in calories is achieved by a reduction

in the amount of carbohydrate.

Further, in summary, for almost every nutrient the CR feed, F05314,

contains 67% more grams of nutrient per kilogram of feed than does

the control feed, # F05312. This includes 67% more casein (the major

protein source) and 67% more soybean oil (the principal source of

fat). The result is that when one feeds 40% less (grams) of F05314

the mice are eating the same amounts of all the above nutrients as

the control mice.

There are four nutrients which, in the CR feed, are not 67% higher

(grams of nutrient per kilogram of feed) than in the control diet.

They are starch, dextrin, fiber and sucrose. To cut a longish story

short it appears that the CR mice consumed 98% less starch; 55% less

dextrin; 14% less fibre; and 20% more (sic) sucrose (compared with

the control mice, in absolute terms).

As a reminder ......... after going on this 43.9% CR diet at

age 60’-65', in a two step process, the mice lived 42% longer than

those eating a 10% CR diet - equivalent, perhaps, to a ~twelve year

difference in humans.

Quite interesting.

Rodney.

=========================

On 16 Apr 2004, Rodney Nicholson wrote:

Hi folks:

OK. I got permission to reproduce the data here. So here they are

for a start. More information (analysis) in subsequent posts.

Rodney.

The following data, for the control and CR diets in Dr. Spindler's

very recently published study, is very kindly provided by:

Yisheng Bai. Ph.D.

Director of Nutrition

Bio-Serv

One 8th Street, Suite One

Frenchtown, NJ 08825

PH: 908-996-2155 ext. 117

PH: 800-996-9908 ext. 117

FAX: 908-996-4123

Ingredient List

Bio-Servís Formulas of F05312, F05314, F05553

Dustless Precision Pellets, 1 gm, Rodent Diet, AIN-93M

Control (F05312), 40% DR (F05314)

F05312 F05314

Ingredient gm/kg gm/kg

Casein 140.00 233.33

Sucrose 100.00 200.00

Fiber 40.00 57.80

Dextrin 470.00 355.00

Starch 160.70 5.00

Salt Mix (AIN-93MMX) 35.00 58.30

Soybean oil 40.00 66.70

Vitamin Mix (AIN-93MVX)10.00 16.70

L-Cystine 1.80 3.00

Choline Bitartrate 2.50 4.20

-------------------------

1. Mineral Premix, AIN-93MMX 2. Vitamin Premix, AIN-93MVX

Ingredient (g/kg mix) Ingredient (g/kg mix)

Calcium carbonate, anhydrous 357.00 Nicotinic acid 3.000

Potassium phosphat, monobasic 250.00 Calcium pantothenate 1.600

Potassium sulfate 46.60 Pyridoxine-HCl 0.700

Potassium citrate tri-K, H2O 28.00 Thiamin-HCl 0.600

Sodium chloride 74.00 Riboflavin 0.600

Magnesium oxide 24.00 Folic acid 0.200

Ferric citrate 6.06 d-Biotin 0.020

Zinc carbonate 1.65 Vitamin B12(0.1% mannitol)

Manganous carbonate 0.63 2.500

Cupric carbonate 0.30 Vitamin E acetat(500 IU/g)

15.000

Potasium iodate 0.01 Vitamin A palmitate

Sodium selenate, anhydrate 0.01025 (500K IU/g) 0.800

Ammonium paramolybdate, 4H2O 0.00795 Vitamin D3(400K IU/g)0.250

Sodium meta-silicate, 9H2O 1.4500 Vitamin K1 0.075

Chromium potassium sulfate, 0.2750 Powdered sucrose 974.655

Lithium chloride 0.0174

Boric acid 0.0815

Sodium fluoride 0.0635

Nickel carbonate 0.0318

Ammonium vanadate 0.0066

Powdered sucrose 209.8060

Prepared by Dr. Yisheng Bai, Bio-Serv

Revised date: 2/7/02

Reproduced with permission from the CR Support List at:

/

[Guest guest]

Hi Warren:

Thanks for that editing, which I can see makes things a bit clearer!

My next post will try to clarify what is in the mouse diet data

tables.

Rodney.

>

> Hi folks:

>

> OK. I got permission to reproduce the data here. So here they

are

> for a start. More information (analysis) in subsequent posts.

>

> Rodney.

>

> The following data, for the control and CR diets in Dr. Spindler's

> very recently published study, is very kindly provided by:

>

> Yisheng Bai. Ph.D.

> Director of Nutrition

> Bio-Serv

> One 8th Street, Suite One

> Frenchtown, NJ 08825

> PH: 908-996-2155 ext. 117

> PH: 800-996-9908 ext. 117

> FAX: 908-996-4123

>

> Ingredient List

> Bio-Servís Formulas of F05312, F05314, F05553

>

> Dustless Precision Pellets, 1 gm, Rodent Diet, AIN-93M

> Control (F05312), 40% DR (F05314)

>

> F05312 F05314

> Ingredient gm/kg gm/kg

> Casein 140.00 233.33

> Sucrose 100.00 200.00

> Fiber 40.00 57.80

> Dextrin 470.00 355.00

> Starch 160.70 5.00

> Salt Mix (AIN-93MMX) 35.00 58.30

> Soybean oil 40.00 66.70

> Vitamin Mix (AIN-93MVX)10.00 16.70

> L-Cystine 1.80 3.00

> Choline Bitartrate 2.50 4.20

>

> -------------------------

> 1. Mineral Premix, AIN-93MMX 2. Vitamin Premix, AIN-93MVX

> Ingredient (g/kg mix) Ingredient (g/kg mix)

> Calcium carbonate, anhydrous 357.00 Nicotinic acid 3.000

> Potassium phosphat, monobasic 250.00 Calcium pantothenate 1.600

> Potassium sulfate 46.60 Pyridoxine-HCl 0.700

> Potassium citrate tri-K, H2O 28.00 Thiamin-HCl 0.600

> Sodium chloride 74.00 Riboflavin 0.600

> Magnesium oxide 24.00 Folic acid 0.200

> Ferric citrate 6.06 d-Biotin 0.020

> Zinc carbonate 1.65 Vitamin B12(0.1% mannitol)

> Manganous carbonate 0.63 2.500

> Cupric carbonate 0.30 Vitamin E acetat(500 IU/g)

> 15.000

> Potasium iodate 0.01 Vitamin A palmitate

> Sodium selenate, anhydrate 0.01025 (500K IU/g) 0.800

> Ammonium paramolybdate, 4H2O 0.00795 Vitamin D3(400K IU/g)0.250

> Sodium meta-silicate, 9H2O 1.4500 Vitamin K1 0.075

> Chromium potassium sulfate, 0.2750 Powdered sucrose 974.655

> Lithium chloride 0.0174

> Boric acid 0.0815

> Sodium fluoride 0.0635

> Nickel carbonate 0.0318

> Ammonium vanadate 0.0066

> Powdered sucrose 209.8060

>

> Prepared by Dr. Yisheng Bai, Bio-Serv

>

> Revised date: 2/7/02

>

> Reproduced with permission from the CR Support List at:

> /

[Guest guest]

Hi Warren/folks:

This is an attempt to clarify the data tables. And guess what? I

have just discovered that the way to see the tables much better

formatted is NOT to read them in the regular window, BUT INSTEAD TO

CLICK THE REPLY BUTTON AND READ THEM IN THE REPLY WINDOW AS IF YOU

ARE ABOUT TO SEND IN A RESPONSE!!!

Try it (try clicking the reply button before reading the post) and

see if it works (i.e. is much better formatted) the same way for you

as it does for me.

Rodney.