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Proteomics. 2006 Jan 10; [Epub ahead of print] Related Articles, Links

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Comparative proteomic analysis of Helicobacter pylori strains associated

with iron deficiency anemia.

Park SA, Lee HW, Hong MH, Choi YW, Choe YH, Ahn BY, Cho YJ, Su Kim D,

Lee NG.

Department of Bioscience and Biotechnology, Institute of Bioscience,

Sejong University, Seoul, Korea.

Helicobacter pylori is known to cause

chronic gastritis,

peptic ulcer, and

gastric cancer, and has also been linked to

iron deficiency anemia (IDA).

To determine whether H. pylori clinical isolates correlate with the

prevalence of H. pylori-associated iron deficiency anemia, we compared the

proteomic profiles of H. pylori strains isolated from antral biopsy

specimens of H. pylori-positive patients with or without iron deficiency

anemia. Fifteen strains, including eight non-iron deficiency anemia and

seven iron deficiency anemia strains, were cultured under iron-rich and

iron-depleted conditions and then analyzed for protein expression profiles

by 2-DE. The distances between two H. pylori strains were determined on the

basis of similarities between their expression patterns of 189 protein

spots, and a phylogenetic tree was constructed.

The results revealed that the iron deficiency anemia strains formed a

cluster separate from that of six non-iron deficiency anemia strains, with

two non-iron deficiency anemia strains between the clusters. H. pylori

strain 26695 was located in the non-iron deficiency anemia cluster. Protein

spots displaying similar expression patterns were clustered, and 18 spots

predominantly expressed in iron deficiency anemia strains were identified by

MALDI-TOF analysis.

These data indicate that the non-iron deficiency anemia and iron deficiency

anemia strains can be distinguished by their protein expression profiles,

suggesting that the polymorphism of H. pylori strains may be one of the

factors determining the occurrence of H. pylori-associated iron deficiency

anemia.

PMID: 16404725 [PubMed - as supplied by publisher]