Inhibition of patulin production by Penicillium expansum cultured with neem

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http://www3.interscience.wiley.com/cgi-

bin/abstract/107642618/ABSTRACT

Journal of Basic Microbiology

Volume 44, Issue 2 , Pages 106 - 113

Published Online: 1 Apr 2004

Copyright © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Original Paper

Inhibition of patulin production by Penicillium expansum cultured

with neem (Azadirachta indica) leaf extracts

Simone Aparecida Galerani Mossini, Kesia Pires De Oliveira,

Kemmelmeier, Dr. *

Department of Biochemistry, Universidade Estadual de Maringá,

Maringá PR Brazil

email: Kemmelmeier (ckemmelmeier@...)

*Correspondence to Kemmelmeier, Fax: (+5544) 263-3655

Abstract

Aqueous extract of the leaves of neem [Azadirachta indica A. Juss

(Meliaceae)] was tested in vitro for antifungal activity against

Penicillium expansum. Patulin production was inhibited during

cultivation, when concentrations higher than 50 mg/ml of neem leaf

extract was added to the culture medium. Analyses of mycotoxin

production were performed by TLC[Note ][Abbreviations used: TLC,

Thin layer chromatography; HPLC, High-performance liquid

chromatography; UEM, Universidade Estadual de Maringá [state

University of Maringá]; IAPAR, Instituto Agrônomico do Paraná

[Agronomy Institute of Paraná].] and HPLC. Fungal growth and colony

characteristics, in the presence of the extract, were investigated

and compared with extract-free cultures. (© 2004 WILEY-VCH Verlag

GmbH & Co. KGaA, Weinheim)

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