Detection methods of ZAP-70 in chronic lymphocytic leukemia

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BlankDetection methods of ZAP-70 in chronic lymphocytic leukemia.

YH Wang, L Fan, W Xu, and JY Li

Clin Exp Med, June 21, 2011;

Department of Hematology, The First Affiliated Hospital of Nanjing Medical

University, Jiangsu Province Hospital, 300 Guangzhou Road, Nanjing, 210029,

China.

The clinical course of patients with chronic lymphocytic leukemia (CLL) is

highly heterogeneous, with some patients experiencing rapid disease progression

and others living for decades without requiring treatment. Immunoglobulin heavy

chain variable region (IGHV) mutation status is a powerful prognostic factor in

patients with CLL. The presence or absence of IGHV mutation status is currently

the gold-standard prognostic factor, but this technique is labor-intensive and

costly. The expression of ?-chain-associated protein kinase 70?kDa (ZAP-70),

detected by flow cytometry, has been served as a potential surrogate for the

absence of IGHV mutation status since 2003. Given the current problems with the

standardization of ZAP-70 assessment by flow cytometry, this chapter reviews the

analysis of ZAP-70 expression by flow cytometry and the relative experimental

conditions using the associated publications for " ZAP-70 " and " detection method "

on Medline since 2003. Thus, mean fluorescence intensity (MFI) ratio method

yielded a more reproducible and easily adaptable method to routine use in the

clinical diagnostic laboratory. In conclusion, though ZAP-70 is a valuable

prognostic factor in CLL, detection methods of ZAP-70 remain to be fully

standardized.

PMID: 21691729