Apoptosis induced by Magnolia Grandi?ora extract in chlorambucil-resistant B-chronic lymphocytic leukemia cells

[Guest guest]

BlankApoptosis induced by Magnolia Grandi?ora extract in chlorambucil-resistant

B-chronic lymphocytic leukemia cells.

GH Marin and E Mansilla

J Cancer Res Ther, October 1, 2010; 6(4): 463-5.

Department of Pharmacology and Clinical, Faculty of Medical Sciences, National

University of La Plata, Buenos Aires, Argentina.

Background: B-cell chronic lymphocitic leukemia (B-CLL) still remains as an

uncurable disease. Even the newest antineoplastic agents have demonstrated

limitations in their efficacy. For this reason, further research of new

compounds must be done. New pharmacological properties can be obtained from a

great diversity botanical species. Among these products, Magnolia Grandiflora

receives our attention since it mainly contains Honokiol which had demonstrated

effect against B-CLL cells activating different cell death pathways. Aim: To

test the ability of Magnolia Grandiflora extracts to induce apoptosis of B-CLL

cells in vitro. Materials and Methods: Herb's extraction: Twenty grams of

powdered material were submitted to three consecutives decoctions with 500 ml of

distilled water (96 ?C), filtered and followed by ultrafiltration with cellulose

membrane, lyophilized and reconstituted in AIM-V medium at a final concentration

of 10 mg/ml solution. B-CLL chlorambucil- resistant cells were separated and

cultivated in the presence of Magnolia's extract. Samples of cells were taken

from the cultures at 24, 48 and 72 h for apoptosis analysis by flow cytometry

measuring positive annexin V (0.1 ?g/ml) cells. Statistics: Apoptosis values

were represented by the mean plus or minus SD (? SD) for five independent

experiments. Statistical significance was determined by Student's t -test. A P

value of 0.05 or less was considered as significant. Results and Conclusion:

This article discusses the apoptosis properties of Magnolia on B-CLL cells. The

evidence suggests a potentially effective repertoire for B-CLL treatment. This

herb extract might have promising therapy strategies in treating B-CLL or other

hematological disease resistant to alkylating agents in clinical practice.

PMID: 21358081