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Hi All,

Hi All,

Cat intermittent feeding is studied in the pdf-available below paper.

Impact of time-limited feeding and dietary carbohydrate content on weight loss

in

group-housed cats

Journal of Feline Medicine & Surgery, In Press, Corrected Proof, Available

online 22

June 2005,

E. Michel, Amy Bader, Frances S. Shofer, Barbera, Donna A.

Oakley

and Urs Giger

SummaryPlus | Full Text + Links | PDF (149 K)

Excessive weight gain is a major problem for the pet cat population and

considered

the most common nutritionally related disease in this species (Lund et al 1999).

Two

epidemiological studies conducted in the United States in the 1990s estimated

that

approximately 25% of the feline population was overweight or obese based on body

condition scoring (BCS) (Scarlett and Donoghue, 1996 and Lund et al., 1999). A

number of factors are thought to contribute to weight gain in pet cats.

Neutering, a

common practice particularly in the United States, has been found by several

investigators to predispose cats to gain weight due to a decrease in energy

expenditure and an increase in food intake (Flynn et al., 1996 and Fettman et

al.,

1997). Indoor confinement is also thought to lead to further decreased energy

expenditure because of exercise restriction. The feeding of commercial pet foods

has

also been implicated, as pet food manufacturers have been successful in

producing

highly palatable commercial cat foods. Free choice feeding of dry (extruded) cat

food is a common practice, especially in multi-cat households, as it facilitates

the

natural feeding behavior of the domestic cat, which is to eat many small meals

dispersed throughout the day and night (Mungford and Thorne, 1980 and Kane et

al.,

1981). Hence, the ad libitum availability of palatable, often energy-dense dry

cat

foods may allow cats to consume energy in excess of their actual needs leading

to

progressive weight gain. Furthermore, weight control in multi-cat households can

be

problematic as it is often not possible to feed cats separately. Thus, it is not

feasible to feed different diets to different cats or control food intake on an

individual basis.

Recently, there has been speculation that the relatively high carbohydrate

content

of dry cat foods, typically 30–40% of calories, could also be a contributing

factor

to weight gain in pet cats. Low carbohydrate diets as a means of promoting

weight

reduction for overweight people are currently receiving much attention although

the

efficacy of this approach remains unproven (Bravata et al 2003). While the

actual

mechanism by which carbohydrate intake would facilitate weight gain in cats has

not

been elucidated, it is well known that feline energy metabolism is uniquely

adapted

to a diet that contains little, if any, carbohydrate. Whether feeding a low

carbohydrate diet would protect against weight gain in cats by inducing satiety

or

altering metabolism has not been demonstrated, although anecdotal reports

suggest

this might be the case.

This study was intended to investigate two interventions meant to prevent weight

gain and promote weight loss in a colony of group-housed cats. The principal aim

was

to investigate the effect of feeding a dry therapeutic feline diet that

contained

less carbohydrate than typical commercial dry cat foods. A secondary aim was to

investigate the feasibility of transitioning these group-housed cats from ad

libitum

feeding to time-limited feeding, where access to food was restricted to 4 h a

day.

The impact of these interventions on body weight (BW) and body condition was

evaluated.

Materials and methods

.... The cats had been fed a commercial HC, high fiber cat food (HC: Hill's

Science

Diet Feline Light Adult-Dry, Hill's Pet Nutrition) (Table 1) and water ad

libitum.

Food was offered in multiple large bowls to provide ample access to all cats.

The

cat colony was gradually transitioned over 2 weeks to have food access

restricted to

4 h/day. Initially, this process involved making food available in the morning

and

then removing any remaining food 12 h later. After 4 days, food availability was

limited to 8 h/day for another 4 days before the final transition to 4 h/day

access.

After the cats were acclimatized to 4 h/day food access, they were randomized

into

two groups of 12 each based on BW, BCS and length of time in the colony. The

cats in

the HC group were continued on the reduced energy, high fiber diet while the

cats in

the LC group were transitioned to a dry therapeutic low carbohydrate cat food

(LC:

Purina DM Feline Formula Dry, Nestle-Purina Co) over the course of 3 weeks

(Table

1). ... all cats were monitored to insure that they were eating and not losing

weight at a too rapid rate (>3% BW/week).

Table 1. Nutrient composition of study dietsa

............................................

Diet Protein (g/kg) Fat (g/kg) NFEb (g/kg) Crude fiber (g/kg) Energy (MJ/kg)

............................................

HCc 333 87 378 58 13.4

LCd 533 165 138 11.5 17.2

...........................................

a Manufacturer's data.

b NFE; Nitrogen-free extract

c Hill's Science Diet Feline Light Adult-Dry, Hill's Pet Nutrition, Inc, Topeka,

KS.

d Purina DM Feline Formula Dry, Nestle-Purina Co, St. Louis, MO.

Immediately following the transition period, Trial 1 was begun. Both groups of

cats

were offered equal amounts of food, by weight, each morning at approximately

08.00

and any remaining food was removed 4 h later and weighed on a gram scale. Daily

food

intake for each group was the difference between the food offered and the food

remaining after 4 h of access. ... Each cat was also assigned a BCS using a

9-point

system based on assessment of body silhouette and adipose tissue by palpation at

the

time of weighing (Laflamme 1997). The amount of food the cats had consumed on a

daily basis prior to the initiation of this investigation was unknown as they

had

been fed ad libitum and also the number of cats in the colony was increased by

six

just before the study began. Therefore, the amount of food the cats were offered

each day was based on the quantity they had consumed in the 12-h period of

time-limited feeding plus an additional 15% by weight. Trial 1 lasted 13 weeks.

Within 2 weeks of the onset of Trial 1 the cats were consuming all of the food

that

was offered in less than 4 h. Consequently, because of the difference in the

energy

density of the two diets, the cats in the LC group received on an average 0.703

MJ/BWkg2/3, while the cats of the HC group received an average of 0.574

MJ/BWkg2/3.

This discrepancy prompted a second phase of investigation, where both groups of

cats

were fed the LC diet. Prior to the initiation of Trial 2, the HC group cats were

accustomed to the LC diet over a 2-week period. Then this group, now referred to

as

the LC/High Calorie group, was offered the same amount of the LC diet, by

weight,

which the LC group had received during Trial 1; the result was that they

received on

an average 0.749 MJ/BWkg2/3. In contrast, the cats in the LC group had their

food

portion restricted to reflect the same amount of energy the HC group had

received

during Trial 1. They were renamed as the LC/Low Calorie group and received on an

average 0.574 MJ/BWkg2/3. The cats were fed and monitored in the same manner as

in

Trial 1 for 12 weeks. Fig 1 shows a schematic representation of the study

design.

.... Results

At the beginning of the investigation, the 18 cats who had been members of the

colony for 2–3 years weighed 7.23±1.29 kg (mean±SD) and had a median BCS of 7/9

(range: 5–9). Based on previously recorded weights, the cats had gained an

average

of 0.1 kg/month up until the commencement of the investigation. The six newly

introduced cats weighed 5.11±0.48 kg (mean±SD) and had a median BCS of 5.5/9

(range:

5–6). There were no statistically significant differences in BW, BCS, or time in

the

colony between the two diet groups at baseline (Table 2).

Table 2. Characteristics of cats in the LC and HC groups at time of study entry

..........................................

----LC group HC group

..........................................

Body weight (kg)a 6.45±1.36 6.44±1.51

Body condition score (BCS)b 7.0 (5–9) 7.0 (5–9)

Time in the colony of resident cats (months)a 30±3 31±1

Cats

Total 12 12

Resident cats 9 9

New cats 3 3

.......................................

LC = a dry low carbohydrate diet; HC = a dry reduced energy diet.

a Mean±SD.

b Median (range).

During the transition to time-limited feeding cats gradually adjusted their food

consumption so that by the end of the 2 weeks they were consuming greater

amounts of

food in 4 h than they initially did in 12 h (Fig 2). All but two cats

experienced

weight loss with an average loss of 1.9±1.7% or 0.13±0.11 kg of BW/week

(mean±SD)

(Fig 3). Only one cat lost weight in excess of 3% BW/week. The cats in this

investigation appeared to tolerate this feeding practice in that they all

readily

adapted by eating as soon as food was made available and remained in good health

throughout the study.

At the end of Trial 1, the cats in the LC group did not experience a significant

change in weight compared to baseline (-0.05±0.09 kg, P = 0.61, vs baseline),

whereas cats in the HC group lost 0.35±0.09 kg (P = 0.03, vs baseline) (Fig 4).

Additionally, the LC and HC diet groups differed from one another with regard to

BW

change from week 2 onwards (P = 0.01 for week 2 and P < 0.0001 for weeks 3–13,

between group comparisons). When cats were stratified based on body condition,

normal condition cats in the LC group (BCS = 6) gained 0.11±0.13 kg (P = 0.43,

vs

baseline), while overweight cats in the LC group (BCS > 6) lost 0.16±0.11 kg (P

=

0.20, vs baseline). These changes, however, were not significantly different

from

baseline for either body condition subgroup. For the cats in the HC group, the

normal condition cats lost 0.1±0.13 kg (P = 0.50, vs baseline) while the

overweight

cats lost 0.52±0.08 kg (P = 0.0005, vs baseline). Only the overweight cats in

the HC

group differed significantly in BW change from the other three body

condition/diet

groups (P < 0.01 for all comparisons with other diet groups) (Fig 5). Neither

group

experienced a significant change in median BCS from the start of Trial 1.

During Trial 2, LC/Low Calorie cats lost 0.37±0.12 kg (P = 0.0087, vs baseline),

whereas LC/High Calorie cats gained 0.24±0.11 kg (P = 0.044, vs baseline). The

low

and high energy intake groups differed from one another with regard to BW

changes

from week 1 onwards (P = 0.005 for week 1 and P < 0.0001 for weeks 2–12,

comparisons

between groups) (Fig 6). When cats were stratified based on body condition, the

weight of the normal condition cats in LC/Low Calorie group remained unchanged

(-0.01±0.11 kg (P = 0.96, vs baseline)), while overweight LC/Low Calorie cats

lost

0.62±0.10 kg (P = 0.0008, vs baseline). For the LC/High Calorie cats, the normal

condition cats gained 0.68±0.05 kg (P = 0.0007, vs baseline), while the

overweight

cats remained unchanged (0.02±0.08 kg (P = 0.77, vs baseline)). Within the

LC/High

Calorie group, normal condition cats differed significantly from overweight cats

with regard to BW change beginning in week 1 (P < 0.0001 for all weeks,

comparisons

between groups). Similarly, within the LC/Low Calorie group, normal condition

cats

differed significantly from overweight cats with regard to BW change beginning

in

week 2 (P < 0.0004 for weeks 2–3 and P < 0.0001 for weeks 4–12, comparisons

between

groups) (Fig 7). Neither group experienced a significant change in median BCS

from

the start of Trial 2.

Discussion

In this investigation all cats initially lost weight when their access to food

was

restricted from ad libitum feeding to 4 h/day. However, they quickly adapted to

the

time-limited feeding and further weight changes reflected the total energy

offered/day. On an average, when these cats had their energy intake restricted

to

the same degree, they lost weight at the same rate regardless of whether they

received the conventional high fiber, low energy food or the low carbohydrate

formulation. No change in BCS was noted. This was likely because the magnitude

of

the weight loss seen within the relatively brief duration of the trial was not

sufficient for most cats to experience a change in their BCS.

It has been reported that about 25% of cats in the United States have an

overweight

or obese body condition (BCS > 6/9) and that for cats between the age of 5 and

12

years, the prevalence of this problem increases to 45% (Armstrong and Lund,

1996,

Scarlett and Donoghue, 1996 and Lund et al., 1999). An overweight or obese body

condition has been associated with increased risk for health problems in cats

including diabetes mellitus, idiopathic hepatic lipidosis, dermatological

disorders,

and lameness (Burrows et al., 1981 and Scarlett and Donoghue, 1996).

There has been much speculation about the causes of weight gain with neutering,

confinement to indoor housing, and ad libitum feeding of palatable cat food

commonly

cited as contributing factors. Ad libitum feeding offers convenience to cat

owners,

particularly in multi-cat households, and also permits a more natural feeding

behavior in that cats typically eat 10–12 small meals spaced throughout a 24-h

period (Mungford and Thorne, 1980 and Kane et al., 1981). However, ad libitum

feeding also permits a cat to consume energy in excess of its daily requirement.

Furthermore, the ad libitum feeding method relies on dry cat foods as canned cat

foods are not stabilized or preserved for prolonged exposure at room

temperature.

Nearly all dry cat foods are manufactured by extrusion, a process that typically

makes use of a formulation containing carbohydrate in the form of starches.

To date, despite great popularity, the efficacy of low carbohydrate diets for

weight

loss in humans remains unproven, particularly over the long term (>6 months)

(

et al 2003). A recent meta-analysis of 94 investigations of low carbohydrate

diets

in humans concluded that participant weight loss was principally associated with

decreased energy intake and increased diet duration but not with reduced dietary

carbohydrate content (Bravata et al 2003). Aside from the current popularity of

low

carbohydrate diets for weight reduction in humans, recognition that the domestic

cat, due to its metabolic adaptations as a strict carnivore, has no dietary

requirement for carbohydrate has led to growing speculation that conventional

dry

cat foods may predispose to weight gain and that a low carbohydrate diet might

facilitate weight loss in this species. Potential mechanisms by which a low

carbohydrate diet might facilitate weight loss in cats include the simulation of

a

fasting condition, whereby blood glucose concentrations are maintained through

hepatic glucose synthesis (a process that requires energy) resulting in energy

wasting. Alternatively some have speculated that low carbohydrate diets may

promote

greater satiety, thus decreasing voluntary food intake. However, the authors are

unaware of any investigations of the efficacy of low carbohydrate diets for

promoting weight loss or satiety in cats.

A limitation of this investigation was that the food intake of each individual

cat

could not be measured because the cats were group-housed and could not be fed

separately. This constraint, however, is commonly encountered in households with

more than one pet cat. Therefore, while this aspect of the study design limited

interpretation of our observations, it did allow evaluation of the practicality

and

impact of time-limited feeding the various diets in a setting more analogous to

multi-cat households than that of cats housed individually in cages. While this

study was not designed to evaluate the impact of time-limited feeding on feline

behavior, the cats in this investigation appeared to tolerate this feeding

practice

in that they all readily adapted by eating as soon as food was made available

and

remained in good health throughout the study.

Satiety cannot be assessed in a straightforward and objective fashion in

animals. It

can be inferred by measuring food intake in ad libitum feeding situations or

measuring consumption of a test meal offered in addition to the normal diet

(Jewell

and Toll, 1996, Butterwick and Markwell, 1997 and et al., 1997). The

cats in

this study were not offered food in excess of what they were willing to consume

and,

therefore, we were unable to draw any conclusions about any impact of dietary

formulation on satiety.

When the cats were stratified according to body condition, cats with normal BCS

either maintained or gained weight depending on the amount of energy offered. In

contrast, the overweight cats lost weight when their energy intake was reduced

regardless of which diet was fed. Because the individual food intake of each cat

was

not measured, the reason for this discrepancy is not known. However, assuming

that

on an average, the cats were able to consume similar amounts of food during the

time

it was available, the overweight cats would have received fewer MJ/BWkg2/3 than

the

normal condition cats. While it is true that the overweight cats should require

fewer MJ/BWkg2/3 because much of their excess weight is less metabolically

active

adipose tissue, the difference was sufficient to lead to weight loss when the

total

energy provided was restricted.

The study design allowed for crossover in energy intake between the two groups

of

cats but not diet. Neither group was fed the HC diet in energy equivalent to the

amount that the LC group in Trial 1 and LC/High Calorie group in Trial 2

received.

However, historically, the long-term members of the colony had gained weight on

this

diet when fed ad libitum. There does remain the possibility that the diet

sequence

may have affected our findings. While the normal weight cats in the HC group did

maintain weight during Trial 1, they did experience energy restriction compared

to

the cats in the LC group. Therefore, it is possible that they adapted to that

level

of energy intake, and when their diet was liberalized, this resulted in the

weight

gain that was observed.

One final limitation in the study design was that the diets used were

commercially

available cat foods and differed in both nutrient profile and ingredients.

Therefore, in addition to dissimilar carbohydrate content, these diets also

contained differing amounts of protein, fat and fiber. The diets also differed

in

energy density. Therefore, the observations of this investigation must be viewed

within the context of the diets used. Specifically formulated test diets and

appropriate study design could overcome the disparity in ingredients and control

for

nutrient profile.

In conclusion, body condition and energy intake but not type of diet influenced

weight loss in this cohort of group-housed cats. Future studies to investigate

whether dietary carbohydrate intake affects food intake and feeding behavior

including satiety are warranted.

Al Pater, PhD; email: old542000@...

__________________________________________________

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