Guest guest Posted June 14, 2005 Report Share Posted June 14, 2005 No. But do a medline search for yourself. You'll find stuff like this: Mol Cancer. 2005 Jan 11;4(1):1. Related Articles, Links Dietary exposure to soy or whey proteins alters colonic global gene expression profiles during rat colon tumorigenesis. Xiao R, Badger TM, Simmen FA. Arkansas Children's Nutrition Center, 1120 Marshall Street, Little Rock, AR 72202, USA. XiaoRijin@... BACKGROUND: We previously reported that lifetime consumption of soy proteins or whey proteins reduced the incidence of azoxymethane (AOM)-induced colon tumors in rats. To obtain insights into these effects, global gene expression profiles of colons from rats with lifetime ingestion of casein (CAS, control diet), soy protein isolate (SPI), and whey protein hydrolysate (WPH) diets were determined. RESULTS: Male Sprague Dawley rats, fed one of the three purified diets, were studied at 40 weeks after AOM injection and when tumors had developed in some animals of each group. Total RNA, purified from non-tumor tissue within the proximal half of each colon, was used to prepare biotinylated probes, which were hybridized to Affymetrix RG_U34A rat microarrays containing probes sets for 8799 rat genes. Microarray data were analyzed using DMT (Affymetrix), SAM (Stanford) and pair-wise comparisons. Differentially expressed genes (SPI and/or WPH vs. CAS) were found. We identified 31 induced and 49 repressed genes in the proximal colons of the SPI-fed group and 44 induced and 119 repressed genes in the proximal colons of the WPH-fed group, relative to CAS. Hierarchical clustering identified the co-induction or co-repression of multiple genes by SPI and WPH. The differential expression of I-FABP (2.92-, 3.97-fold down-regulated in SPI and WPH fed rats; P = 0.023, P = 0.01, respectively), cyclin D1 (1.61-, 2.42-fold down-regulated in SPI and WPH fed rats; P = 0.033, P = 0.001, respectively), and the c-neu proto-oncogene (2.46-, 4.10-fold down-regulated in SPI and WPH fed rats; P < 0.001, P < 0.001, respectively) mRNAs were confirmed by real-time quantitative RT-PCR. SPI and WPH affected colonic neuro-endocrine gene expression: peptide YY (PYY) and glucagon mRNAs were down-regulated in WPH fed rats, whereas somatostatin mRNA and corresponding circulating protein levels, were enhanced by SPI and WPH. CONCLUSIONS: The identification of transcripts co- or differentially-regulated by SPI and WPH diets suggests common as well as unique anti-tumorigenesis mechanisms of action which may involve growth factor, neuroendocrine and immune system genes. SPI and WPH induction of somatostatin, a known anti-proliferative agent for colon cancer cells, would inhibit tumorigenesis. PMID: 15644144 [PubMed - in process] On 6/14/05, Francesca Skelton <fskelton@...> wrote: > Where did this come from? Sounds like something that might have been > written by Whey Protein Manufacturers.......... > > > on 6/14/2005 10:09 AM, Dowling at > christopher.a.dowling@... wrote: > > > Casein may be bad stuff, on its own, but its bad effects may not apply > > to other proteins: > > > > > > " Fighting Cancer With Whey " > > > > Studies on whey demonstrate it's an even better protein supplement > > than previously thought. Although whey protein's health benefits have > > only recently been elucidated, the use of whey protein for medicinal > > purposes has been prescribed since the time of Hippocrates. In fact, > > there are two ancient proverbs from the Italian city of Florence that > > say, " If you want to live a healthy and active life, drink whey, " and, > > " If everyone were raised on whey, doctors would be bankrupt. " > > > > > > > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted June 14, 2005 Report Share Posted June 14, 2005 And this: Int J Toxicol. 2001 May-Jun;20(3):165-74. Related Articles, Links Developmental effects and health aspects of soy protein isolate, casein, and whey in male and female rats. Badger TM, Ronis MJ, Hakkak R. Arkansas Children's Nutrition Center and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock 72202, USA. badgerthomasm@... Dietary factors other than the traditional nutrients are found in the so-called functional foods. They are becoming increasingly recognized as potentially important for maintaining good health. Soybeans are rich in such factors thought to help prevent certain chronic diseases. Soy protein isolate (SPI) is one of the three major proteins used in infant formulas sold in the United States, with casein (CAS) and whey (WPH) proteins being the others. We have been studying the health effects of these proteins. Safety concerns have developed over the consumption of soy-based infant formula, partly because of the high circulating levels of the total isoflavones (phytoestrogens) during " critical periods of infant development. " There is a paucity of data on developmental, physiological, neurophysiological, behavioral, metabolic, or molecular effects of soy phytochemicals in humans, especially during pregnancy and infancy. We have studied the effects of CAS, SPI, and WPH in short-term, long-term, and multigenerational studies in rats. Aside from minor differences in body weight gain profiles, CAS-, SPI- or WPH-fed rats did not differ in development, organ weights, in vitro hepatic metabolism of testosterone (T), or reproductive performance. However, some endocrine-related functions differed between rats fed these proteins. We found that SPI accelerated puberty in female rats (p < .05) and WPH delayed puberty in males and females, as compared with CAS (p < .05). Gender differences were also found in gonadectomy-induced steroid responses. Male rats had normal serum T levels, but female rats fed SPI had reduced serum 17beta-estradiol concentrations and a blunted 17beta-estradiol response to ovariectomy, as compared to rats fed CAS or WHP (p < .05). Female rats fed SPI or WHP or treated with genistein had reduced incidence of chemically induced mammary cancers (p < .05) compared to CAS controls, with WHP reducing tumor incidence by as much as 50%, findings that replicate previous results from our laboratory. Together, these results suggest gender-specific differences in development and certain endocrine responses among rats fed diets composed of a single protein source such as those used in infant formulas. Whether similar developmental effects occur in human infants is unknown, but unlikely because (1) most infants do not consume such diets throughout life as these rats did, and (2) no such effects have been reported in millions of American infants fed infant formula containing these proteins. The long-term health consequence implications of early diet exposure to SPI and WPH, such as reduced breast cancer incidence, are likely to be very positive. PMID: 11488559 [PubMed - indexed for MEDLINE] On 6/14/05, Dowling <christopher.a.dowling@...> wrote: > No. But do a medline search for yourself. You'll find stuff like this: > > Mol Cancer. 2005 Jan 11;4(1):1. Related Articles, Links > > > Dietary exposure to soy or whey proteins alters colonic global gene > expression profiles during rat colon tumorigenesis. > > Xiao R, Badger TM, Simmen FA. > > Arkansas Children's Nutrition Center, 1120 Marshall Street, Little > Rock, AR 72202, USA. XiaoRijin@... > > BACKGROUND: We previously reported that lifetime consumption of soy > proteins or whey proteins reduced the incidence of azoxymethane > (AOM)-induced colon tumors in rats. To obtain insights into these > effects, global gene expression profiles of colons from rats with > lifetime ingestion of casein (CAS, control diet), soy protein isolate > (SPI), and whey protein hydrolysate (WPH) diets were determined. > RESULTS: Male Sprague Dawley rats, fed one of the three purified > diets, were studied at 40 weeks after AOM injection and when tumors > had developed in some animals of each group. Total RNA, purified from > non-tumor tissue within the proximal half of each colon, was used to > prepare biotinylated probes, which were hybridized to Affymetrix > RG_U34A rat microarrays containing probes sets for 8799 rat genes. > Microarray data were analyzed using DMT (Affymetrix), SAM (Stanford) > and pair-wise comparisons. Differentially expressed genes (SPI and/or > WPH vs. CAS) were found. We identified 31 induced and 49 repressed > genes in the proximal colons of the SPI-fed group and 44 induced and > 119 repressed genes in the proximal colons of the WPH-fed group, > relative to CAS. Hierarchical clustering identified the co-induction > or co-repression of multiple genes by SPI and WPH. The differential > expression of I-FABP (2.92-, 3.97-fold down-regulated in SPI and WPH > fed rats; P = 0.023, P = 0.01, respectively), cyclin D1 (1.61-, > 2.42-fold down-regulated in SPI and WPH fed rats; P = 0.033, P = > 0.001, respectively), and the c-neu proto-oncogene (2.46-, 4.10-fold > down-regulated in SPI and WPH fed rats; P < 0.001, P < 0.001, > respectively) mRNAs were confirmed by real-time quantitative RT-PCR. > SPI and WPH affected colonic neuro-endocrine gene expression: peptide > YY (PYY) and glucagon mRNAs were down-regulated in WPH fed rats, > whereas somatostatin mRNA and corresponding circulating protein > levels, were enhanced by SPI and WPH. CONCLUSIONS: The identification > of transcripts co- or differentially-regulated by SPI and WPH diets > suggests common as well as unique anti-tumorigenesis mechanisms of > action which may involve growth factor, neuroendocrine and immune > system genes. SPI and WPH induction of somatostatin, a known > anti-proliferative agent for colon cancer cells, would inhibit > tumorigenesis. > > PMID: 15644144 [PubMed - in process] > > > On 6/14/05, Francesca Skelton <fskelton@...> wrote: > > Where did this come from? Sounds like something that might have been > > written by Whey Protein Manufacturers.......... > > > > > > on 6/14/2005 10:09 AM, Dowling at > > christopher.a.dowling@... wrote: > > > > > Casein may be bad stuff, on its own, but its bad effects may not apply > > > to other proteins: > > > > > > > > > " Fighting Cancer With Whey " > > > > > > Studies on whey demonstrate it's an even better protein supplement > > > than previously thought. Although whey protein's health benefits have > > > only recently been elucidated, the use of whey protein for medicinal > > > purposes has been prescribed since the time of Hippocrates. In fact, > > > there are two ancient proverbs from the Italian city of Florence that > > > say, " If you want to live a healthy and active life, drink whey, " and, > > > " If everyone were raised on whey, doctors would be bankrupt. " > > > > > > > > > > > > > Quote Link to comment Share on other sites More sharing options...
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