Chromosomal study for prognostic grouping in chronic lymphocytic leukemia.

[Guest guest]

BlankChromosomal study for prognostic grouping in chronic lymphocytic leukemia.

A Junaid, PN Rao, and MM Adil

J Coll Physicians Surg Pak, January 1, 2011; 21(1): 19-22.

Department of Pathology, Shifa Inernational Hospital, Islamabad.

Objective: To determine the frequency of various cytogenetic aberrations in

newly diagnosed chronic lymphocytic leukemia (CLL) patients, and their detection

rate by cytogenetic and fluorescent In situ hybridization (FISH) technique

separately. Study Design: A case series. Place and Duration of Study: Clinical

and Molecular Cytogenetics Laboratories, University of California, Los Angeles,

USA, from November 2007 to July 2008. Methodology: Analysis was made on 100

diagnosed chronic lymphocytic leukemia patients. Cytogenetics and FISH technique

were performed on blood or bone marrow samples. Results: Nineteen out of 100

cases (19%) showed karyotype abnormalities; whereas 55 showed abnormalities

using the CLL - specific FISH probes. The most frequent abnormality detected by

standard cytogenetics was trisomy 12. The most common abnormality detected by

FISH was a deletion of 13q14 (40 out of 55 cases; 72% of the abnormal).

Conclusion: For prognostic grouping of CLL patients, FISH must always be

requested which may even replace standard karyotyping. These chromosomal markers

help in choosing the therapeutic options.

PMID: 21276379