Anti-mutagenic and Pro-apoptotic Effects of Apigenin on Human Chronic Lymphocytic Leukemia Cells

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BlankAnti-mutagenic and Pro-apoptotic Effects of Apigenin on Human Chronic

Lymphocytic Leukemia Cells.

M Hashemi, M Nouri Long, M Entezari, S Nafisi, and H Nowroozii

Acta Med Iran, September 1, 2010; 48(5): 283-8.

Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran,

Iran.

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The purpose of this research is to examine anti-mutagenic and apoptotic effects

of apigenin in human lymphoma cells. In present study human chronic lymphocytic

leukemia (Eheb cell line) were cultured in RPMI 1640 (Sigma), supplemented with

10% fetal calf serum, penicillin-streptomycin, L-glutamine and incubated at 37

?C for 2 days. In addition cancer cell line was treated by and apigenin and

cellular vital capacity was determined by MTT assay. Then effect of apigenin in

human lymphoma B cells was examined by flow cytometry techniques. The apigenin

was subsequently evaluated in terms of anti-mutagenic properties by a standard

reverse mutation assay (Ames test). This was performed with histidine auxotroph

strain of Salmonella typhimurium (TA100). Thus, it requires histidine from a

foreign supply to ensure its growth. The aforementioned strain gives rise to

reverted colonies when expose to sodium azide as a carcinogen substance. During

MTT assay, human chronic lymphocytic leukemia revealed to have a meaningful cell

death when compared with controls (P<0.01) Apoptosis was induced suitably after

48 hours by flow cytometry assay. In Ames test apigenin prevented the reverted

mutations and the hindrance percent of apigenin was 98.17%.These results have

revealed apigenin induced apoptosis in human lymphoma B cells in vitro.

PMID: 21287458