Flow-cytometry detection of minimal DNA aneuploidy in mature lymphoid leukemias - comparison with metaphase and interphase cytogenetics.

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BlankFlow-cytometry detection of minimal DNA aneuploidy in mature lymphoid

leukemias - comparison with metaphase and interphase cytogenetics.

S Kamihira, E Matutes, J Marco, S Hoda, R Morilla, K Owusuankomah, A Crowford, J

Ellis, and D Catovsky

Int J Oncol, August 1, 1994; 5(2): 211-4.

INST CANC RES,LONDON SW3 6JJ,ENGLAND. ROYAL MARSDEN HOSP,ACAD DEPT HAEMATOL &

CYTOGENET,LONDON SW3 6JJ,ENGLAND.

The relative DNA content of peripheral blood cells from 79 cases with lymphoid

leukemias was analyzed by a dual-parameter flow cytometric analysis. The

leukemia samples corresponded to: chronic lymphocytic leukemia (CLL) 40, CLL

with more than 10% prolymphocytes (CLL/PL) 12, CLL mixed 9, prolymphocytic

leukemia (PLL) 5, and B-cell lymphoma in leukemic phase 13. DNA aneuploidy was

found overall in 26 (32.9%) of the cases and these corresponded to: 7 (17.5%)

with CLL, 7 (58.3%) with CLL/PL, 4 (44.4%) with CLL mixed, 2 (40%) with PLL and

6 (46.2%) with B-cell lymphoma. There was a good correlation between DNA content

and cytogenetics/fluorescent in situ hybridization in all but 2 cases as

follows: 6 of 7 cases with diploid DNA had normal karyotype and only one had

trisomy 12: 4 of 6 cases with hyperdiploid DNA had trisomy 12, one had

tetraploidy and only one had a normal karyotype. Two cases were hypodiploid both

by DNA and cytogenetic analysis. Our findings demonstrate a higher incidence of

DNA aneuploidy in B-cell lymphoma in leukemic phase, PLL, and atypical CLL in

comparison with typical CLL and a good correlation with cytogenetics. We

conclude that flow cytometric DNA analysis represents a useful, sensitive, and

rapid method to detect and monitor minimal changes of DNA content in leukemic

lymphocytes without the need of short-term cultures.

PMID: 21559577

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On May 21, 2011, at 9:31 AM, Gach wrote:

BlankFlow-cytometry detection of minimal DNA aneuploidy in mature

lymphoid leukemias - comparison with metaphase and interphase

cytogenetics.

S Kamihira, E Matutes, J Marco, S Hoda, R Morilla, K Owusuankomah, A

Crowford, J Ellis, and D Catovsky

Int J Oncol, August 1, 1994; 5(2): 211-4.

INST CANC RES,LONDON SW3 6JJ,ENGLAND. ROYAL MARSDEN HOSP,ACAD DEPT

HAEMATOL & CYTOGENET,LONDON SW3 6JJ,ENGLAND.

The relative DNA content of peripheral blood cells from 79 cases with

lymphoid leukemias was analyzed by a dual-parameter flow cytometric

analysis. The leukemia samples corresponded to: chronic lymphocytic

leukemia (CLL) 40, CLL with more than 10% prolymphocytes (CLL/PL) 12,

CLL mixed 9, prolymphocytic leukemia (PLL) 5, and B-cell lymphoma in

leukemic phase 13. DNA aneuploidy was found overall in 26 (32.9%) of

the cases and these corresponded to: 7 (17.5%) with CLL, 7 (58.3%)

with CLL/PL, 4 (44.4%) with CLL mixed, 2 (40%) with PLL and 6 (46.2%)

with B-cell lymphoma. There was a good correlation between DNA content

and cytogenetics/fluorescent in situ hybridization in all but 2 cases

as follows: 6 of 7 cases with diploid DNA had normal karyotype and

only one had trisomy 12: 4 of 6 cases with hyperdiploid DNA had

trisomy 12, one had tetraploidy and only one had a normal karyotype.

Two cases were hypodiploid both by DNA and cytogenetic analysis. Our

findings demonstrate a higher incidence of DNA aneuploidy in B-cell

lymphoma in leukemic phase, PLL, and atypical CLL in comparison with

typical CLL and a good correlation with cytogenetics. We conclude that

flow cytometric DNA analysis represents a useful, sensitive, and rapid

method to detect and monitor minimal changes of DNA content in

leukemic lymphocytes without the need of short-term cultures.

PMID: 21559577