Re: Lactoferrin is low in chronic rhino sinusitis, especially w/biofilms

[Guest guest]

Lactoferrin (LF), also known as lactotransferrin (LTF), is a globular multifunctional protein with antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. Lactoferrin is found in milk and many mucosal secretions such as tears and saliva. Lactoferrin is also present in secondary granules of PMN and also is secreted by some acinar cells. Lactoferrin can be purified from milk or produced recombinantly. Human colostrum has the highest concentration, followed by human milk, then cow milk. This is cut from the wikipedia definition.

I had been dairy free since I was 20 (44 now). I read that yogurt had very little lactose, so this year I started making and eating it daily. I have been feeling well but there are other changes that could be credited as well, (aciphex and aspirin).

Good information, thank you,

Pam

On 5/4/08 2:06 PM, " asfyso " <asfyso@...> wrote:

This abstract says that patients with chronic rhinosinusitis have low lactoferrin levels, and even more so if they have biofilms in their sinuses. The authors conclude that some people may have naturally low lactoferrin levels, which might predispose them to biofilms (but the abstract doesn't say if, on the other hand, biofilm colonization reduces lactoferrin levels). Anyway, this may provide a rationale for supplementing in lactoferrin (check for side effects first).

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Reduced Levels of Lactoferrin in Biofilm-Associated Chronic Rhinosinusitis.

Sinonasal

Laryngoscope. 118(5):895-901, May 2008.

Psaltis, Alkis J. MBBS; Wormald, - MD; Ha, Kien R. MBBS; Tan, Lor Wai PhD

Abstract:

Objective/Hypothesis: The diverse antipathogenic action of lactoferrin has been well characterized. In addition, it is the human body's only known antimicrobial peptide with antibiofilm properties. The purpose of this study was to examine the nasal mucosal expression of lactoferrin in the biofilm-mediated disease, chronic rhinosinusitis (CRS).

Study Design/Methods: Nasal biopsies from 41 CRS patients and 21 healthy controls were analyzed using confocal scanning laser microscopy (CSLM) and scanning electron microscopy (SEM) for the presence of biofilms. The messenger ribonucleic acid (mRNA) and protein level of lactoferrin in this tissue were also determined by quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) and enzyme linked immunosorbent assay (ELISA), respectively.

Results: Lactoferrin expression in chronic rhinosinusitis patients at both mRNA and protein level was downregulated relative to controls. Biofilm-positive CRS patients showed a much greater reduction in lactoferrin expression than biofilm-negative patients; mRNA median fold change biofilm positive = 0.03 (interquartile range 0.005-0.15) and biofilm-negative CRS median fold change = 0.49 (interquartile range 0.15-0.81) with median lactoferrin protein expression biofilm-positive patients' median lactoferrin protein expression = 32.58 ng/mL (interquartile range 8.67-59.9 ng/mL) and biofilm-negative patients' median lactoferrin expression = 114.40 ng/mL (interquartile range 75.41-163.1 ng/mL).

Conclusions: Genetic, transcriptional, or translational deficiencies in lactoferrin synthesis may reduce the functional level of this important antimicrobial/antibiofilm peptide in the nasal secretions of CRS patients, predisposing certain individuals to bacterial colonization, biofilm development, and recalcitrant sinus disease.

© The American Laryngological, Rhinological & Otological Society, Inc.

[Guest guest]

To the mothers-to-be, this is another reason to breast-feed!

On 5/4/08 2:44 PM, " Pam " <pam@...> wrote:

Lactoferrin (LF), also known as lactotransferrin (LTF), is a globular multifunctional protein with antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. Lactoferrin is found in milk and many mucosal secretions such as tears and saliva. Lactoferrin is also present in secondary granules of PMN and also is secreted by some acinar cells. Lactoferrin can be purified from milk or produced recombinantly. Human colostrum has the highest concentration, followed by human milk, then cow milk. This is cut from the wikipedia definition.

I had been dairy free since I was 20 (44 now). I read that yogurt had very little lactose, so this year I started making and eating it daily. I have been feeling well but there are other changes that could be credited as well, (aciphex and aspirin).

Good information, thank you,

Pam

On 5/4/08 2:06 PM, " asfyso " <asfyso@...> wrote:

This abstract says that patients with chronic rhinosinusitis have low lactoferrin levels, and even more so if they have biofilms in their sinuses. The authors conclude that some people may have naturally low lactoferrin levels, which might predispose them to biofilms (but the abstract doesn't say if, on the other hand, biofilm colonization reduces lactoferrin levels). Anyway, this may provide a rationale for supplementing in lactoferrin (check for side effects first).

--------

Reduced Levels of Lactoferrin in Biofilm-Associated Chronic Rhinosinusitis.

Sinonasal

Laryngoscope. 118(5):895-901, May 2008.

Psaltis, Alkis J. MBBS; Wormald, - MD; Ha, Kien R. MBBS; Tan, Lor Wai PhD

Abstract:

Objective/Hypothesis: The diverse antipathogenic action of lactoferrin has been well characterized. In addition, it is the human body's only known antimicrobial peptide with antibiofilm properties. The purpose of this study was to examine the nasal mucosal expression of lactoferrin in the biofilm-mediated disease, chronic rhinosinusitis (CRS).

Study Design/Methods: Nasal biopsies from 41 CRS patients and 21 healthy controls were analyzed using confocal scanning laser microscopy (CSLM) and scanning electron microscopy (SEM) for the presence of biofilms. The messenger ribonucleic acid (mRNA) and protein level of lactoferrin in this tissue were also determined by quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) and enzyme linked immunosorbent assay (ELISA), respectively.

Results: Lactoferrin expression in chronic rhinosinusitis patients at both mRNA and protein level was downregulated relative to controls. Biofilm-positive CRS patients showed a much greater reduction in lactoferrin expression than biofilm-negative patients; mRNA median fold change biofilm positive = 0.03 (interquartile range 0.005-0.15) and biofilm-negative CRS median fold change = 0.49 (interquartile range 0.15-0.81) with median lactoferrin protein expression biofilm-positive patients' median lactoferrin protein expression = 32.58 ng/mL (interquartile range 8.67-59.9 ng/mL) and biofilm-negative patients' median lactoferrin expression = 114.40 ng/mL (interquartile range 75.41-163.1 ng/mL).

Conclusions: Genetic, transcriptional, or translational deficiencies in lactoferrin synthesis may reduce the functional level of this important antimicrobial/antibiofilm peptide in the nasal secretions of CRS patients, predisposing certain individuals to bacterial colonization, biofilm development, and recalcitrant sinus disease.

© The American Laryngological, Rhinological & Otological Society, Inc.