Re: Researchers working on the identification of biofilms

[Guest guest]

Has anyone used H and E stains with a light microscope to look at microorganisms

in sinus samples(ie from colored or unusual discharge when a sinus is cleared).

>

> Eur Arch Otorhinolaryngol. <http://www.ncbi.nlm.nih.gov/pubmed/21547386>

> 2011 May 6. [Epub ahead of print]Biofilm detection in chronic

> rhinosinusitis by combined application of hematoxylin-eosin and gram

> staining.Tóth L

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22T%C3%B3th%20L%22%5BAuthor%5D\

> > , Csomor P

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Csomor%20P%22%5BAuthor%5D> ,

> Sziklai I

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Sziklai%20I%22%5BAuthor%5D>

> , Karosi T

> <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Karosi%20T%22%5BAuthor%5D>

> .Source

> Department of Otolaryngology and Head and Neck Surgery, University of

> Debrecen, Medical and Health Science Center, Nagyerdei Krt. 98,

> Debrecen, 4032, Hungary.

> Abstract

> The pathomechanism of chronic rhinosinusitis with nasal polyposis

> (CRS/NP) seems to be unclear. Bacterial-, fungal- and combined biofilms

> might play a potential role in the pathogenesis of various inflammatory

> diseases and recently in CRS/NP. A prospective, blinded observational

> study was performed to confirm that the combination of conventional

> hematoxylin-eosin (HE) and Gram staining protocols could be used to

> detect bacterial and fungal biofilms in patients with CRS/NP. A total of

> 50 patients with CRS/NP undergoing endoscopic sinus surgery (ESS) were

> analyzed. The negative control group consisted of 12 patients undergoing

> septoplasty for nasal obstruction without CRS/NP. The nasal polyps and

> inferior turbinate mucosa specimens applied as negative controls were

> processed to HE and Gram staining. Biofilm was detected in 44 of 50

> patients with CRS/NP and in none of 12 negative controls. In our series,

> HE method showed an obvious correlation with the results of Gram

> staining and was allocated to be a good predictor of biofilm existence.

> It was found that the microscopic structure and thickness of biofilms

> were strongly associated with the integrity of nasal mucosa and with the

> characteristics of subepithelial cellular infiltration. This study

> confirmed the presence of bacterial and fungal biofilms on the surface

> of NPs obtained from patients with CRS. Since biofilms may affect the

> severity and recurrence rate of CRS treated by ESS they should be

> detected histologically. In conclusion, HE staining combined with Gram

> protocol is a robust and reliable method for the detection of bacterial

> and fungal biofilms in CRS/NP.

>