Re: My Latest F.I.S.H results

[Guest guest]

Gale,

I am so happy to see a FISH report like this. Your are blessed.

The only problem is...I am somewhat envious..my last test two months

ago was at 13% (working for ZERO)...have my fingers and toes crossed.

I am awaiting for the results from my last test...hope to have it

today or no later than monday, April 4th. My prayers for you will

be to remain in this condition until a complete cure comes in the

form of pill....to seek out those last few lurking leukie cells.

Warm Regards,

Jim Beck - Pennsylvania, USA

dx 8/2001

chronic - WBC 497,000 - 100% PH+

Interferon, Hydrea

IM - 2/2002 - 400, 500, 600mg Up & down

currently 400mg w/pancytopenia

FISH 13% - Jan 2005

>

> Hello All,

>

> Here's my latest FISH report. The details are here in case

someone

> hasn't seen this before:

>

> BCR-ABL T(9,22) FUSION 0.000

> The real time assay shows no evidence of expression of the BCR-ABL

> fusion transcript which usually results from t(9,22) (q34;q11)

> translocation. This assay is a real-time quantitative RT-PCR

assay.

> Extracted sample RNA is subjected to a real-time RT-PCR reaction

to

> measure the quantity of the two types of BCR-ABL fusion

transcripts

> (b2a2/b3a2 and e1a2). An additional amplification for ABL gene is

> performed as a control for sample RNA quality.

> <blah, blah, blah ...>

> Then, it says: The analytical sensitivity of this test is 1 tumor

> cell in 100,000 normal cells.

>

> So, I'm happy to report that I'm still a big fat zero too!

> (I'm sure there are a few lurking leukemia around, but I'm glad

> they are just lurking...)

>

> To your good blood,

> Gale Bacon

[Guest guest]

Hi Gale,

These results are actually from a PCR test and not a FISH test but all

the more encredible since PCR's are more sensitive than FISH's.

Congratulations,

Tracey

[Guest guest]

Congratulations, Gale. Celebrate.

Annette

>

> Hello All,

>

> Here's my latest FISH report. The details are here in case

someone

> hasn't seen this before:

>

> BCR-ABL T(9,22) FUSION 0.000

> The real time assay shows no evidence of expression of the BCR-ABL

> fusion transcript which usually results from t(9,22) (q34;q11)

> translocation. This assay is a real-time quantitative RT-PCR

assay.

> Extracted sample RNA is subjected to a real-time RT-PCR reaction

to

> measure the quantity of the two types of BCR-ABL fusion

transcripts

> (b2a2/b3a2 and e1a2). An additional amplification for ABL gene is

> performed as a control for sample RNA quality.

> <blah, blah, blah ...>

> Then, it says: The analytical sensitivity of this test is 1 tumor

> cell in 100,000 normal cells.

>

> So, I'm happy to report that I'm still a big fat zero too!

> (I'm sure there are a few lurking leukemia around, but I'm glad

> they are just lurking...)

>

> To your good blood,

> Gale Bacon

[Guest guest]

Hi Gale,

Great to hear your last test came back at zero. It looks like one

more thing we have in common, still Zero's. Keep up the good work!

Take care,

#425 Zavie's Zero Club

>

> Hello All,

>

> Here's my latest FISH report. The details are here in case

someone

> hasn't seen this before:

>

> BCR-ABL T(9,22) FUSION 0.000

> The real time assay shows no evidence of expression of the BCR-ABL

> fusion transcript which usually results from t(9,22) (q34;q11)

> translocation. This assay is a real-time quantitative RT-PCR

assay.

> Extracted sample RNA is subjected to a real-time RT-PCR reaction

to

> measure the quantity of the two types of BCR-ABL fusion

transcripts

> (b2a2/b3a2 and e1a2). An additional amplification for ABL gene is

> performed as a control for sample RNA quality.

> <blah, blah, blah ...>

> Then, it says: The analytical sensitivity of this test is 1 tumor

> cell in 100,000 normal cells.

>

> So, I'm happy to report that I'm still a big fat zero too!

> (I'm sure there are a few lurking leukemia around, but I'm glad

> they are just lurking...)

>

> To your good blood,

> Gale Bacon

[Guest guest]

Hi Gale,

That's great news.

Here's to lot's more big fat zeros!!!

Sharon

-- In , " Gale " <divergal@a...> wrote:

>

> Hello All,

>

> Here's my latest FISH report. The details are here in case

someone

> hasn't seen this before:

>

> BCR-ABL T(9,22) FUSION 0.000

> The real time assay shows no evidence of expression of the BCR-ABL

> fusion transcript which usually results from t(9,22) (q34;q11)

> translocation. This assay is a real-time quantitative RT-PCR

assay.

> Extracted sample RNA is subjected to a real-time RT-PCR reaction

to

> measure the quantity of the two types of BCR-ABL fusion

transcripts

> (b2a2/b3a2 and e1a2). An additional amplification for ABL gene is

> performed as a control for sample RNA quality.

> <blah, blah, blah ...>

> Then, it says: The analytical sensitivity of this test is 1 tumor

> cell in 100,000 normal cells.

>

> So, I'm happy to report that I'm still a big fat zero too!

> (I'm sure there are a few lurking leukemia around, but I'm glad

> they are just lurking...)

>

> To your good blood,

> Gale Bacon

[Guest guest]

Hello Gale,

How nice to see you are still PCRU!

I was thinking about SCUBA diving today - still way too cold for that

here, but it is nice to think about it!

Cheers,

Cheryl-Anne

>

> Hello All,

>

> Here's my latest FISH report. The details are here in case someone

> hasn't seen this before:

>

> BCR-ABL T(9,22) FUSION 0.000

> The real time assay shows no evidence of expression of the BCR-ABL

> fusion transcript which usually results from t(9,22) (q34;q11)

> translocation. This assay is a real-time quantitative RT-PCR

assay.

> Extracted sample RNA is subjected to a real-time RT-PCR reaction to

> measure the quantity of the two types of BCR-ABL fusion transcripts

> (b2a2/b3a2 and e1a2). An additional amplification for ABL gene is

> performed as a control for sample RNA quality.

> <blah, blah, blah ...>

> Then, it says: The analytical sensitivity of this test is 1 tumor

> cell in 100,000 normal cells.

>

> So, I'm happy to report that I'm still a big fat zero too!

> (I'm sure there are a few lurking leukemia around, but I'm glad

> they are just lurking...)

>

> To your good blood,

> Gale Bacon