Nasal absorption and biodistribution of plasmid DNA: an alternative route of DNA vaccine delivery

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I send these out as information only, not as saying I agree - just so you

know what they are doing out there.

Soon they won't even need your permission (and probably now) - just spray

your neighborhood, your school, whatever.

Sheri

http://www.elsevier.nl/gej-ng/10/42/35/40/47/43/abstract.html

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Vaccine, Vol. 19 (31) (2001) pp. 4519 - 4525

=A9 2001 Elsevier Science Ltd. All rights reserved.=20

PII: S0264-410X(01)00188-8

Nasal absorption and biodistribution of plasmid DNA: an alternative route

of DNA vaccine delivery

Yu-Kyoung Oh a * ohyk@... , Jong-Pil Kim a, Tae Sun Hwang b, Jung

Jae Ko a, Jung Mogg Kim c, Ji-Sun Yang d and Chong-Kook Kim e

a Department of Microbiology and Institute of Medical Research, College of

Medicine, Pochon CHA University, Kyonggi-do 487-800, South Korea

b Department of Anatomy, College of Medicine, Pochon CHA University,

Kyonggi-do 487-800, South Korea

c Department of Microbiology and Institute of Biomedical Science, College

of Medicine, Hanyang University, Seoul, South Korea

d Department of Pharmacology, Korea Food and Drug Administration, Seoul,

South Korea

e College of Pharmacy, Seoul National University, Seoul, South Korea=20

Received 1 February 2001; received in revised form 9 May 2001; accepted 9

May 2001

Abstract

Nasal administration is emerging as a new route of DNA vaccine delivery. We

aimed to study the extent of absorption and biodistribution of intranasally

administered plasmid DNA. After intranasal administration, the level of

plasmid DNA in the serum peaked at 1.5 h. The ratio of the area under the

concentration (AUC) after intranasal administration of DNA over the AUC

after intravenous administration was 0.14. At 15 min post inoculation, the

highest organ distribution was observed in the liver and the cervical lymph

nodes showed the highest level among the lymph nodes. At 24 h a higher

localization of plasmids to the brain than to the lung and spleen was

notable. A significant level of mRNA expression was observed in the lymph

nodes. These results suggest that plasmid DNA can be substantially absorbed

and distributed to the lymph nodes after intranasal administration, partly

explaining the systemic immunogenicity of intranasally administered plasmid

DNA vaccines.

Keywords: Plasmid DNA; Nasal absorption; Distribution

*Corresponding author. Tel.: +82-31-542-6671; fax: +82-31-543-2818=20

[Full text] (PDF 206.5 Kb)=20

=A9 Copyright 2001, Elsevier Science, All rights reserved.

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Sheri Nakken, R.N., MA=09

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http://www.nccn.net/~wwithin/vaccine.htm

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