Glutathione levels in antigen presenting cells modulates a Th1 versus Th2 response

[Guest guest]

This was posted some time ago, but I have only got

around to reading it recently.

http://www.pnas.org/cgi/content/full/95/6/3071?maxtoshow= & HITS=10 & hits=10 & RESULT\

FORMAT= & author1=%2C+J & searchid=QID_NOT_SET & FIRSTINDEX=

This paragraph seemed interesting, some apparently

irreparable damage due to only short term depleation

of GSH. What are the implications? Can one restore

IL-12 activity or, if not, try to compensate somehow?

" IL-12 Production Does Not Recover in Parallel with

IFN-g and GSH. The Th1 immune response pattern is

typified by T cell production of IFN-g and APC

production of IL-12. Consistent with this, we find

that loss of IFN-g production because of APC GSH

depletion is accompanied by loss of IL-12 production

(Fig. 3 and Table 2). Surprisingly, however,

we find that although recovery of APC GSH in

DEM-treated cells results in full recovery of support

for IFN-g production, IL-12 production does not

recover. Instead, IL-12 levels in cultures containing

DEM-treated APC that were allowed to recover before T

cell and antigen addition remain low whether tested at

12 (data not shown) or 72 h of culture (Fig. 3). "

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[Guest guest]

,

This IS interesting. Thanks for raising it. It seems to say that an

irreversible change occurred in the antigen-presenting cells that had

been treated with diethyl maleate. I think it's important to note

that this part of the work was done in vitro, with an established cell

population. In the body, there is the possibility of making new

cells. It would have been interesting to restore the glutathione

levels in the mice themselves, and then determine whether they were

able to produce IL-12 again, but it doesn't appear that that was done.

So it's difficult to say from this paper whether this loss of IL-12

production would really be irreversible in an intact body. I

suspect that it wouldn't.

Rich (Just dropping in, still not able to monitor the list

continuously)

> This was posted some time ago, but I have only got

> around to reading it recently.

>

>

http://www.pnas.org/cgi/content/full/95/6/3071?maxtoshow= & HITS=10 & hits

=10 & RESULTFORMAT= & author1=%2C+J & searchid=QID_NOT_SET & FIRSTINDE

X=

>

>

> This paragraph seemed interesting, some apparently

> irreparable damage due to only short term depleation

> of GSH. What are the implications? Can one restore

> IL-12 activity or, if not, try to compensate somehow?

>

> " IL-12 Production Does Not Recover in Parallel with

> IFN-g and GSH. The Th1 immune response pattern is

> typified by T cell production of IFN-g and APC

> production of IL-12. Consistent with this, we find

> that loss of IFN-g production because of APC GSH

> depletion is accompanied by loss of IL-12 production

> (Fig. 3 and Table 2). Surprisingly, however,

> we find that although recovery of APC GSH in

> DEM-treated cells results in full recovery of support

> for IFN-g production, IL-12 production does not

> recover. Instead, IL-12 levels in cultures containing

> DEM-treated APC that were allowed to recover before T

> cell and antigen addition remain low whether tested at

> 12 (data not shown) or 72 h of culture (Fig. 3). "

>

>

> __________________________________________________

>