Guest guest Posted June 10, 2012 Report Share Posted June 10, 2012 1999, Vol. 5, No. 3-4 , Pages 187-197 PDF (131 KB) PDF Plus (132 KB) Reprints Permissions Aristo Vojdani† and Al Franco Immunosciences Laboratory Inc., Beverly Hills, CA, USA Department of Medicine, Drew University School of Medicine and Science, Los Angeles, CA, USA Arthritis Center of Riverside, Riverside, CA, USA †Correspondence: Aristo Vojdani, 8730 Wilshire Boulevard, Suite 305, Beverly Hills, CA, 90211, USA immunsci@... Summary A multiplex polymerase chain reaction (PCR) was used to detect mycoplasma infection in human DNA samples of patients with CFS and related illnesses. One set of oligonucleotide primers which are specific for a highly conserved region among all members of the genusMycoplasma along with three other primer sets which are specific for Mycoplasma fermentans, M. hominis, and M. penetrans species were used in this assay. The sensitivity of detection was determined by adding known mycoplasma DNA copy numbers to 1 & #956;g of genomic DNA from healthy subjects. Each sample was subjected to 40 cycles of amplification. The detection level was determined to be 7, 7, 9, and 15 mycoplasma DNA copies per & #956;g of human genomic DNA forM. genus, M. fermentans, M. hominis, and M. penetrans, respectively. The assay was applied to DNA extracted from the PBMCs of individuals suffering from chronic fatigue syndrome (CFS) (n = 100), fibromyalgia (FMS) (n = 40), rheumatoid arthritis (RA) (n = 60), and gulf war syndrome (GWS) (n = 60) and compared to age- and sex-matched healthy individuals (n = 160). The percentage of M. genus infection detected in CFS, FMS, RA, and GWS was 52,54, 49, and 55%, respectively. M. fermentans was detected in 32, 35, 23, and 36%, M. hominiswas detected in 9, 8,11, and 5%, and M. penetrans was detected in 6, 4, 7, and 3% of CFS, FMS, RA, and GWS patients, respectively. M. genus, M. fermentans, M. hominis, and M. penetrans were detected in 15, 8, 3, and 2% of healthy matched controls. This assay provides a rapid and cost efficient procedure to screen clinical samples for the presence of three potentially pathogenic species of Mycoplasma with a high level of sensitivity and specificity. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted June 10, 2012 Report Share Posted June 10, 2012 Dear Group, Can somebody translate the medical English into everyday English for those of us who do not have a background in biology? I am writing about the summary that Beverly just posted. Mira Quote Link to comment Share on other sites More sharing options...
Recommended Posts
Join the conversation
You are posting as a guest. If you have an account, sign in now to post with your account.
Note: Your post will require moderator approval before it will be visible.