Type 2A overexpression research - from Japan

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Research abstract from Traffic. 2004 Mar;5(3):140-51.

A Novel Kinesin-Like Protein, KIF1Bbeta3 Is Involved in the Movement of

Lysosomes to the Cell Periphery in Non-Neuronal Cells.

Matsushita M, Tanaka S, Nakamura N, Inoue H, Kanazawa H.

Department of Biological Sciences, Graduate School of Science, Osaka

University, Machikaneyama-cho 1-16, Toyonaka City, Osaka, Japan

560-0043.

The kinesin superfamily protein, KIF1Bbeta, a splice variant of KIF1B,

is involved in the transport of synaptic vesicles in neuronal cells, and

is also expressed in various non-neuronal tissues. To elucidate the

functions of KIF1Bbeta in non-neuronal cells, we analyzed the

intracellular localization of KIF1Bbeta and characterized its isoform

expression profile. In COS-7 cells, KIF1B colocalized with lysosomal

markers and expression of a mutant form of KIF1Bbeta, lacking the motor

domain, impaired the intracellular distribution of lysosomes. A novel

isoform of the kinesin-like protein, KIF1Bbeta3, was identified in rat

and simian kidney. It lacks the 5th exon of the KIF1Bbeta-specific tail

region. Overexpression of KIF1Bbeta3 induced the translocation of

lysosomes to the cell periphery. However, overexpression of

KIF1Bbeta3-Q98L, which harbors a pathogenic mutation associated with a

familial neuropathy, Charcot-Marie-Tooth disease type 2 A, resulted in

the abnormal perinuclear clustering of lysosomes. These results indicate

that KIF1Bbeta3 is involved in the translocation of lysosomes from

perinuclear regions to the cell periphery.

PMID: 15086790 [PubMed - in process]