Guest guest Posted February 7, 2012 Report Share Posted February 7, 2012 coliac diseases.reference is skully. From: Areej Abbas <areej.aldura@...> " " < > Sent: Tuesday, 7 February 2012 1:49 PM Subject: which test ELISA test used commonly for the diagnosis of which disease? cheers Quote Link to comment Share on other sites More sharing options...
Guest guest Posted February 7, 2012 Report Share Posted February 7, 2012 Coliac diseases.reference scully From: Areej Abbas <areej.aldura@...> " " < > Sent: Tuesday, 7 February 2012 1:49 PM Subject: which test ELISA test used commonly for the diagnosis of which disease? cheers Quote Link to comment Share on other sites More sharing options...
Guest guest Posted February 7, 2012 Report Share Posted February 7, 2012 many thanks kanika . From: Kanika Kohli <kanika_sahil@...>" " < > Sent: Tuesday, 7 February 2012, 14:05Subject: Re: which test Coliac diseases. reference scully From: Areej Abbas <areej.aldura@...>" " < > Sent: Tuesday, 7 February 2012 1:49 PMSubject: which test ELISA test used commonly for the diagnosis of which disease? cheers Quote Link to comment Share on other sites More sharing options...
Guest guest Posted February 7, 2012 Report Share Posted February 7, 2012 hepatitis From: Kanika Kohli <kanika_sahil@...> " " < > Sent: Tuesday, 7 February 2012, 14:05 Subject: Re: which test Coliac diseases.reference scully From: Areej Abbas <areej.aldura@...> " " < > Sent: Tuesday, 7 February 2012 1:49 PM Subject: which test ELISA test used commonly for the diagnosis of which disease? cheers Quote Link to comment Share on other sites More sharing options...
Guest guest Posted February 7, 2012 Report Share Posted February 7, 2012 For HIV as well. From: Aqsa Fatima Burki <aqsaburki@...> " " < > Sent: Tuesday, 7 February 2012 4:33 PM Subject: Re: which test hepatitis From: Kanika Kohli <kanika_sahil@...> " " < > Sent: Tuesday, 7 February 2012, 14:05 Subject: Re: which test Coliac diseases.reference scully From: Areej Abbas <areej.aldura@...> " " < > Sent: Tuesday, 7 February 2012 1:49 PM Subject: which test ELISA test used commonly for the diagnosis of which disease? cheers Quote Link to comment Share on other sites More sharing options...
Guest guest Posted February 8, 2012 Report Share Posted February 8, 2012 Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool for determining serum antibody concentrations (such as with the HIV test[8] or West Nile Virus). It has also found applications in the food industry in detecting potential food allergens such as milk, peanuts, walnuts, almonds, and eggs.[9] ELISA can also be used in toxicology as a rapid presumptive screen for certain classes of drugs. ELISA (Enzyme-Linked Immunosorbent Assay) plate. The ELISA was the first screening test widely used for HIV because of its high sensitivity. In an ELISA, a person's serum is diluted 400-fold and applied to a plate to which HIV antigens are attached. If antibodies to HIV are present in the serum, they may bind to these HIV antigens. The plate is then washed to remove all other components of the serum. A specially prepared "secondary antibody" — an antibody that binds to other antibodies — is then applied to the plate, followed by another wash. This secondary antibody is chemically linked in advance to an enzyme. Thus, the plate will contain enzyme in proportion to the amount of secondary antibody bound to the plate. A substrate for the enzyme is applied, and catalysis by the enzyme leads to a change in color or fluorescence. ELISA results are reported as a number; the most controversial aspect of this test is determining the "cut-off" point between a positive and a negative result. A cut-off point may be determined by comparing it with a known standard. If an ELISA test is used for drug screening at workplace, a cut-off concentration, 50 ng/mL, for example, is established, and a sample that contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the ELISA test to detect various kind of diseases, such as Malaria, Chagas' disease, and e disease.[10] ELISA tests also are used as in in vitro diagnostics in medical laboratories. The other uses of ELISA include: § detection of mycobacterial antibodies in tuberculosis. § detection of rotavirus in feces. § detection of hepatitis B markers in the serum. § detection of enterotoxin of E. coli in feces. http://en.wikipedia.org/wiki/ELISA cheerskeerthi--- On Tue, 7/2/12, Areej Abbas <areej.aldura@...> wrote:From: Areej Abbas <areej.aldura@...>Subject: which test" " < >Date: Tuesday, 7 February, 2012, 13:49 ELISA test used commonly for the diagnosis of which disease? cheers Quote Link to comment Share on other sites More sharing options...
Guest guest Posted February 8, 2012 Report Share Posted February 8, 2012 cheers keerthi From: keerthi kumar <keerthikumarhs@...> Sent: Wednesday, 8 February 2012, 12:18Subject: Re: which test Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool for determining serum antibody concentrations (such as with the HIV test[8] or West Nile Virus). It has also found applications in the food industry in detecting potential food allergens such as milk, peanuts, walnuts, almonds, and eggs.[9] ELISA can also be used in toxicology as a rapid presumptive screen for certain classes of drugs. ELISA (Enzyme-Linked Immunosorbent Assay) plate. The ELISA was the first screening test widely used for HIV because of its high sensitivity. In an ELISA, a person's serum is diluted 400-fold and applied to a plate to which HIV antigens are attached. If antibodies to HIV are present in the serum, they may bind to these HIV antigens. The plate is then washed to remove all other components of the serum. A specially prepared "secondary antibody" — an antibody that binds to other antibodies — is then applied to the plate, followed by another wash. This secondary antibody is chemically linked in advance to an enzyme. Thus, the plate will contain enzyme in proportion to the amount of secondary antibody bound to the plate. A substrate for the enzyme is applied, and catalysis by the enzyme leads to a change in color or fluorescence. ELISA results are reported as a number; the most controversial aspect of this test is determining the "cut-off" point between a positive and a negative result. A cut-off point may be determined by comparing it with a known standard. If an ELISA test is used for drug screening at workplace, a cut-off concentration, 50 ng/mL, for example, is established, and a sample that contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the ELISA test to detect various kind of diseases, such as Malaria, Chagas' disease, and e disease.[10] ELISA tests also are used as in in vitro diagnostics in medical laboratories. The other uses of ELISA include: § detection of mycobacterial antibodies in tuberculosis. § detection of rotavirus in feces. § detection of hepatitis B markers in the serum. § detection of enterotoxin of E. coli in feces. http://en.wikipedia.org/wiki/ELISA cheers keerthi From: Areej Abbas <areej.aldura@...>Subject: which test" " < >Date: Tuesday, 7 February, 2012, 13:49 ELISA test used commonly for the diagnosis of which disease? cheers Quote Link to comment Share on other sites More sharing options...
Guest guest Posted February 8, 2012 Report Share Posted February 8, 2012 ELISA is a sensitive test for AIDS. is that the specific test?can anybody explain that in detailRegardspadma From: Areej Abbas <areej.aldura@...> " " < > Sent: Wednesday, 8 February 2012, 13:38 Subject: Re: which test cheers keerthi From: keerthi kumar <keerthikumarhs@...> Sent: Wednesday, 8 February 2012, 12:18Subject: Re: which test Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool for determining serum antibody concentrations (such as with the HIV test[8] or West Nile Virus). It has also found applications in the food industry in detecting potential food allergens such as milk, peanuts, walnuts, almonds, and eggs.[9] ELISA can also be used in toxicology as a rapid presumptive screen for certain classes of drugs. ELISA (Enzyme-Linked Immunosorbent Assay) plate. The ELISA was the first screening test widely used for HIV because of its high sensitivity. In an ELISA, a person's serum is diluted 400-fold and applied to a plate to which HIV antigens are attached. If antibodies to HIV are present in the serum, they may bind to these HIV antigens. The plate is then washed to remove all other components of the serum. A specially prepared "secondary antibody" — an antibody that binds to other antibodies — is then applied to the plate, followed by another wash. This secondary antibody is chemically linked in advance to an enzyme. Thus, the plate will contain enzyme in proportion to the amount of secondary antibody bound to the plate. A substrate for the enzyme is applied, and catalysis by the enzyme leads to a change in color or fluorescence. ELISA results are reported as a number; the most controversial aspect of this test is determining the "cut-off" point between a positive and a negative result. A cut-off point may be determined by comparing it with a known standard. If an ELISA test is used for drug screening at workplace, a cut-off concentration, 50 ng/mL, for example, is established, and a sample that contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the ELISA test to detect various kind of diseases, such as Malaria, Chagas' disease, and e disease.[10] ELISA tests also are used as in in vitro diagnostics in medical laboratories. The other uses of ELISA include: § detection of mycobacterial antibodies in tuberculosis. § detection of rotavirus in feces. § detection of hepatitis B markers in the serum. § detection of enterotoxin of E. coli in feces. http://en.wikipedia.org/wiki/ELISA cheers keerthi From: Areej Abbas <areej.aldura@...>Subject: which test" " < >Date: Tuesday, 7 February, 2012, 13:49 ELISA test used commonly for the diagnosis of which disease? cheers Quote Link to comment Share on other sites More sharing options...
Recommended Posts
Join the conversation
You are posting as a guest. If you have an account, sign in now to post with your account.
Note: Your post will require moderator approval before it will be visible.