Olive oil (oleate) saves cardiac cells, saturated fats kill 'em

[Guest guest]

Saturated fats *increase* oxidative stress in biological systems. This can promote apoptosis in susceptible cell populations.

Beyond that, who knows?

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Oleate prevents palmitate-induced cytotoxic stress in cardiac myocytes

Abstract

The cytotoxicity of saturated fatty acids has been implicated in the pathophysiology of cardiovascular disease, though their effects on cardiac myocytes are incompletely understood. We examined the effects of palmitate and the mono-unsaturated fatty acid oleate on neonatal rat ventricular myocyte cell biology. Palmitate (0.5 mM) increased oxidative stress, as well as activation of the stress-associated protein kinases (SAPK) p38, Erk1/2, and JNK, following 18 h and induced apoptosis in 20% of cells after 24 h. Neither antioxidants nor SAPK inhibitors prevented palmitate-induced apoptosis. Low concentrations of oleate (0.1 mM) completely inhibited palmitate-induced oxidative stress, SAPK activation, and apoptosis. Increasing mitochondrial uptake of palmitate with l-carnitine decreased apoptosis, while decreasing uptake with the

carnitine palmitoyl transferase-1 inhibitor perhexiline nearly doubled palmitate-induced apoptosis. These results support a model for palmitate-induced apoptosis, activation of SAPKs, and protein oxidative stress in myocytes that involves cytosolic accumulation of saturated fatty acids.

http://www.sciencedirect.com/science/journal/0006291X

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T. pct35768@...

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[Guest guest]

Here is a better link to this paper. (And by better, I mean actually functional)

http://www.sciencedirect.com/science/journal/0006291X

From there, go to "Articles in Press"

The article below should appear there, about the seventh one down from the top...

Oleate prevents palmitate-induced cytotoxic stress in cardiac myocytes

Abstract

The cytotoxicity of saturated fatty acids has been implicated in the pathophysiology of cardiovascular disease, though their effects on cardiac myocytes are incompletely understood. We examined the effects of palmitate and the mono-unsaturated fatty acid oleate on neonatal rat ventricular myocyte cell biology. Palmitate (0.5 mM) increased oxidative stress, as well as activation of the stress-associated protein kinases (SAPK) p38, Erk1/2, and JNK, following 18 h and induced apoptosis in 20% of cells after 24 h. Neither antioxidants nor SAPK inhibitors prevented palmitate-induced apoptosis. Low concentrations of oleate (0.1 mM) completely inhibited palmitate-induced oxidative stress, SAPK activation, and apoptosis. Increasing mitochondrial uptake of palmitate with l-carnitine decreased apoptosis, while decreasing uptake with the

carnitine palmitoyl transferase-1 inhibitor perhexiline nearly doubled palmitate-induced apoptosis. These results support a model for palmitate-induced apoptosis, activation of SAPKs, and protein oxidative stress in myocytes that involves cytosolic accumulation of saturated fatty acids. Keywords: Lipotoxicity; Palmitate; Apoptosis; Cardiomyocyte; SAPK; Oxidative stress; Oleate

T.

pct35768@...

Start your day with - make it your home page

[Guest guest]

Here is a better link to this paper. (And by better, I mean actually functional)

http://www.sciencedirect.com/science/journal/0006291X

From there, go to "Articles in Press"

The article below should appear there, about the seventh one down from the top...

Oleate prevents palmitate-induced cytotoxic stress in cardiac myocytes

Abstract

The cytotoxicity of saturated fatty acids has been implicated in the pathophysiology of cardiovascular disease, though their effects on cardiac myocytes are incompletely understood. We examined the effects of palmitate and the mono-unsaturated fatty acid oleate on neonatal rat ventricular myocyte cell biology. Palmitate (0.5 mM) increased oxidative stress, as well as activation of the stress-associated protein kinases (SAPK) p38, Erk1/2, and JNK, following 18 h and induced apoptosis in 20% of cells after 24 h. Neither antioxidants nor SAPK inhibitors prevented palmitate-induced apoptosis. Low concentrations of oleate (0.1 mM) completely inhibited palmitate-induced oxidative stress, SAPK activation, and apoptosis. Increasing mitochondrial uptake of palmitate with l-carnitine decreased apoptosis, while decreasing uptake with the

carnitine palmitoyl transferase-1 inhibitor perhexiline nearly doubled palmitate-induced apoptosis. These results support a model for palmitate-induced apoptosis, activation of SAPKs, and protein oxidative stress in myocytes that involves cytosolic accumulation of saturated fatty acids. Keywords: Lipotoxicity; Palmitate; Apoptosis; Cardiomyocyte; SAPK; Oxidative stress; Oleate

T.

pct35768@...

Start your day with - make it your home page