Fat intake counter-CR?

[Guest guest]

Hi All,

Is fat intake counter-CR?

As an introduction to the animal system used in the in press paper, see:

Alliot J, Boghossian S, Jourdan D, Veyrat-Durebex C, Pickering G, Meynial-Denis

D,

Gaumet N.

The LOU/c/jall rat as an animal model of healthy aging?

J Gerontol A Biol Sci Med Sci. 2002 Aug;57(8):B312-20.

PMID: 12145357

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve & db=pubmed & dopt=Abstra\

ct & list_uids=12145357 & query_hl=34

See the not pdf-available below paper.

Fat intake reverses the beneficial effects of low caloric intake on skeletal

muscle

mitochondrial H2O2 production

Free Radical Biology and Medicine, In Press, Corrected Proof, Available online 8

August 2005,

Blandine Garait, Karine Couturier, Stéphane Servais, Dominique Letexier,

Perrin, Cécile Batandier, Jean-Louis Rouanet, Brigitte Sibille, Rey,

Xavier

Leverve and Roland Favier

Abstract

Food restriction is the most effective modulator of oxidative stress and it

is

believed that a reduction in caloric intake per se is responsible for the

reduced

generation of reactive oxygen species (ROS) by mitochondria. Hydrogen peroxide

(H2O2) generation and oxygen consumption (O2) by skeletal muscle mitochondria

were

determined in a peculiar strain of rats (Lou/C) characterized by a

self-low-caloric

intake and a dietary preference for fat. These rats were fed either with a

standard

high-carbohydrate (HC) or a high-fat (HF) diet and the results were compared to

those measured in Wistar rats fed a HC diet. H2O2 production was significantly

reduced in Lou/C rats fed a HC diet; this effect was not due to a lower O2

consumption but rather to a decrease in rotenone-sensitive NADH-ubiquinone

oxidoreductase activity and increased expression of uncoupling proteins 2 and 3.

The

reduced H2O2 generation displayed by Lou/C rats was accompanied by a significant

inhibition of permeability transition pore (PTP) opening. H2O2 production was

restored and PTP inhibition was relieved when Lou/C rats were allowed to eat a

HF

diet, suggesting that the reduced oxidative stress provided by low caloric

intake is

lost when fat proportion in the diet is increased.

Keywords: Self-low-caloric intake; High-fat diet; Membrane potential; Uncoupling

proteins; Calcium retention capacity; Free radicals

Abbreviations: ROS, reactive oxygen species; HC, high carbohydrate; HF, high

fat;

ETC, electron transport chain; PTP, permeability transition pore; EE, energy

expenditure; TEE, total EE; EEdark, EE during the dark phase; BSA, bovine serum

albumin; BCA, bicinchoninic acid; TPMP+, triphenylmethylphosphonium cation;

RT-PCR,

reverse-transcription polymerase chain reaction; T3, triiodothyronine; T4,

tetraiodothyronine; Ca-RC, Ca2+ retention capacity; G/M, glutamate/malate; P/M,

pyruvate/malate; CsA, cyclosporin A; CR, caloric restriction

Al Pater, PhD; email: old542000@...

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