Fasting risk

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Hi All,

Another fasting risk factor may be in the not pdf-available below paper results.

Adachi J, Kudo R, Asano M, Ueno Y, Hunter R, Rajendram R, C, Preedy VR.

Skeletal muscle and liver oxysterols during fasting and alcohol exposure.

Metabolism. 2006 Jan;55(1):119-27.

PMID: 16324930

Oxysterols are cytotoxic agents that have a range of cellular actions, including

impairment of albumin synthesis, cell differentiation, and induction of

apoptosis.

Their regulations by nutritional factors are poorly described. Our objective was

to

test the hypothesis that the imposition of food withdrawal and alcohol exposure

increases tissue oxysterol concentrations. We measured the concentrations of the

oxysterols 7alpha-hydroxycholest-5-en-3beta-ol (7alpha-OH),

7beta-hydroxycholest-5-en-3beta-ol (7beta-OH), and

3beta-hydroxycholest-5-en-7-one

(7-keto) in liver and skeletal muscle of fed and fasted (food withdrawal for 1

and 2

days) male Wistar rats. Both oxidative (type I; soleus) and glycolytic (type II;

plantaris) muscles were analyzed. We also investigated the effects of a

nutritional

perturbant induced by a short-term bolus of ethanol (75 mmol/kg weight IP

administered 2.5 hours before sacrifice). The results showed that in response to

fasting there were significant increases in 7alpha-OH, 7beta-OH, and 7-keto in

liver

and both type I and II skeletal muscle (P < .001 in all instances). For skeletal

muscle, the increases were blunted or ameliorated after 2 days when compared

with

data from rats starved for 1 day. In contrast, the increases in liver after 1

day's

fasting were relatively sustained at 2 days. Short-term ethanol increased

7alpha-OH,

7beta-OH, and 7-keto in type I muscle of fed animals only (P < .001 in all

instances) with a significant interaction between fasting and alcohol (P < .001

in

all instances). For the first time, we have shown that oxysterols can increase

in

muscle and liver in response to food withdrawal and in response to an

immediately

imposed nutritional perturbant (ie, alcohol). Increased oxysterols represent

elevated oxidative stress and/or disturbances in their formation or clearance.

Because of the reported cytotoxic properties of oxysterols, these data are

important

in understanding cellular pathology because episodic anorexia and/or oxidative

stress occur in a variety of disease conditions including sepsis, cancer

cachexia,

ischemia, and hormonal imbalance.

Al Pater, PhD; email: old542000@...

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