Re: Re: My First PCR Results

[Guest guest]

Well, I may be wrong, as it is pretty unclear what the baseline, or

normalizing factor, is. If I have 2 percentages of ph+ cells in my

blood I can certainly compare them. I may be bit fuzzy about the ratio

given in the PCR corresponds really the ratio of ph+ cells in my

blood.

Here what is on my PCR test report (from Stanford Hospital) :

170 p210 copies/ug RNA eqv

Normalized copy number (ratio relative to a normalizing control signal

represents current expression of BCR-ABL in the patient) : 0.0059

log change from diag avg : -2.25

It also says that the log change from Stanford Lab is calculated with

a average for new dx of 1.04 p210 BCR-ABL signal/ABL normalizing

control signal.

This is where it is unclear. 2.25 log reduction is not inconsistent

with a reduction from 100% to 0.59% (2.22 log reduction), so I assumed

so far that it was equivalent to that, and that the 1.04 > 1 takes

into account the false positive. I had 100% ph+ cells at dx. Now,

reading your comment and thinking again about it, if I don't interpret

correctly the 1.04 then it may be a number corresponding to a lower

percentage of ph+ cells, some average of the patients they got, which

I don't know the value. Then my 0.0059 doesn't give directly the ratio

of ph+ cells in my blood and I was wrong, and the actual ratio of ph+

in my blood is lower than 0.0059. If you tell me I have less ph+ cells

in my body than I thought I really wouldn't mind being wrong, even if

I graduated in applied math Looks like I am still learning things

reading this list, over 2 years after beginning

Cheers,

Marcos

On 6/5/07, Tracey <traceyincanada@...> wrote:

>

>

>

>

>

>

>

> > (you didn't tell us your percentage on the FISH test at dx, so I

> can't tell

> > you the reduction).

>

> *******************************

>

> Marcos, how can you compare a FISH test from diagnosis to a PCR test 6

> months later? Isn't that like comparing chocolate muffins to chocolate

> bars? They both contain chocolate but other than that, you can't say

> much about them.

>

> Tracey