RE: Re: Interpretation of PCR Analysis

[Guest guest]

It states a sensitivity level of >1 in 100,000 transcripts. It says Method:

RNA is isolated from the sample provided and converted to cDNA using reverse

transcriptase. The cDNA is amplified by real time polymerase chain reaction

(PCR) for the major and minor BCR_ABL fusion genes.

Of course, this is way outside my scope of knowledge. I just wanted to

confirm this means instead of 100%PH+ chromosomes, I have .006%? Am I

understanding this correctly?

Thanks

Barbara Hundley

Fayetteville, GA

From: [mailto: ] On Behalf Of Tracey

Sent: Thursday, May 24, 2007 8:08 PM

Subject: [ ] Re: Interpretation of PCR Analysis

Hi Barbara,

It looks like the Gleevec is working great! It's hard to compare PCR

values because every lab uses independent methods to do the test but

generally speaking, anything that's as far as the third decimal point

is a GREAT result. You'll have to find out the lab's baseline before

you can be sure but from the surface, I think you should celebrate.

Congratulations,

Tracey

>

> I haven't seen my doctor yet, but did get a copy of my latest PCR

Analysis.

> Wondered if someone could confirm the analysis for me. I'm anxious

to know

> if the Gleevec is working. I've been on 400mg since diagnosed July

06.

>

>

>

> Results: Positive Major breakpoint (0.006%)

>

> Interpretation=this result is consistent with minimal residual

disease

>

>

>

> BCR_ABL transcript

>

> Major a13a2=.006%

>

> Major e14a2=not detected

>

> Major combined=.006%

>

> Minor e1a2=not detected

>

>

>

>

>

> Thanks

>

>

>

>

>

> Barbara

>

> Fayetteville, GA

>

>

>

>

[Guest guest]

0.006% = 0.00006 = 1 ph+ cell in 16666 cells. The text I think just

describes quantitative RT-PCR, the regular routine PCR. That's a

really good result at 1 year, I am jealous .

Marcos.

On 5/24/07, barbara <barb521@...> wrote:

> It states a sensitivity level of >1 in 100,000 transcripts. It says Method:

> RNA is isolated from the sample provided and converted to cDNA using reverse

> transcriptase. The cDNA is amplified by real time polymerase chain reaction

> (PCR) for the major and minor BCR_ABL fusion genes.

>

> Of course, this is way outside my scope of knowledge. I just wanted to

> confirm this means instead of 100%PH+ chromosomes, I have .006%? Am I

> understanding this correctly?

>

> Thanks

>

> Barbara Hundley

>

> Fayetteville, GA

>

>