Need more help in understanding entire test results

[Guest guest]

Hi Tracey,

I got a copy of my bone marrow biopsy test results (in addition to what

I posted here last week). Unfortunately, they are not " bid news " , but I

would be happy to understand what the results tell about the condition

of CML and if I'm moving into right direction and in appropriate pace

(I mean if the reduction that I have is good enough for three months of

treatment or it is kind of slow). This test was taken in three months

after the very first one. I'll really appreciate your feedback on this

one. Here is what I have written on the report:

Clinical Indication: R/O CML

Results: Female Karyotype and Abnormal FISH results positive for cryptic

BCR/ABL gene rearrangement

Interpretation: No clonal cytogenetic abnormalities were detected in

cells from short term unstimulated bone marrow aspirate

cultures at the resolution obtained.

FISH: Of a total of 600 interphase nuclei examined, 23.3% showed an

abnormal positive single fusion signal. The current FISH results

are consistent with continued involvement of bone marrow by the

previously identified cell clone containing cryptic BCR/ABL gene

rearrangement. Compared to the prior specimen (refer to 08/13/08,

abnormal cells = 82.2%), this current specimen shows a decreased

number of abnormal cells.

Cytogenetic Analysis Summary:

Number of Cells Analyzed: 20

Number of Cultures used for Analysis: 2

Number of Cells Karyotyped at Microscope: 14

Number of Hard Copy Karyotypes: 6

Banding Level: 350-450

Banding Method: GTW/G

FISH Analysis Summary:

Number of Cells Analyzed: 600

Cells Analyzed: Interphase

Probes Utilized: BCR/ABL1

Thank you in advance,

Lilia.

[Guest guest]

Hi Lilia,

This is excellent news. It is moving in the right direction and at a very

good pace according to the FISH test..

What I don't see is the cytogenic result. It is normally reported as the

number of cells that are +ive out of 20 cells tested.

What dose of Gleevec are you on?

Zavie

Zavie (age 70)

67 Shoreham Avenue

Ottawa, Canada, K2G 3X3

dxd AUG/99

INF OCT/99 to FEB/00, CHF

No meds FEB/00 to JAN/01

Gleevec since MAR/27/01 (400 mg)

CCR SEP/01. #102 in Zero Club

2.8 log reduction Sep/05

3.0 log reduction Jan/06

2.9 log reduction Feb/07

3.6 log reduction Apr/08

3.6 log reduction Sep/08

e-mail: zmiller@...

Tel: 613-726-1117

Fax: 309-296-0807

Cell: 613-282-0204

ID: zaviem

YM: zaviemiller

Skype: Zavie

_____

From: [mailto: ] On Behalf Of

llokl_777

Sent: November 25, 2008 4:10 PM

Subject: [ ] Need more help in understanding entire test results

Hi Tracey,

I got a copy of my bone marrow biopsy test results (in addition to what

I posted here last week). Unfortunately, they are not " bid news " , but I

would be happy to understand what the results tell about the condition

of CML and if I'm moving into right direction and in appropriate pace

(I mean if the reduction that I have is good enough for three months of

treatment or it is kind of slow). This test was taken in three months

after the very first one. I'll really appreciate your feedback on this

one. Here is what I have written on the report:

Clinical Indication: R/O CML

Results: Female Karyotype and Abnormal FISH results positive for cryptic

BCR/ABL gene rearrangement

Interpretation: No clonal cytogenetic abnormalities were detected in

cells from short term unstimulated bone marrow aspirate

cultures at the resolution obtained.

FISH: Of a total of 600 interphase nuclei examined, 23.3% showed an

abnormal positive single fusion signal. The current FISH results

are consistent with continued involvement of bone marrow by the

previously identified cell clone containing cryptic BCR/ABL gene

rearrangement. Compared to the prior specimen (refer to 08/13/08,

abnormal cells = 82.2%), this current specimen shows a decreased

number of abnormal cells.

Cytogenetic Analysis Summary:

Number of Cells Analyzed: 20

Number of Cultures used for Analysis: 2

Number of Cells Karyotyped at Microscope: 14

Number of Hard Copy Karyotypes: 6

Banding Level: 350-450

Banding Method: GTW/G

FISH Analysis Summary:

Number of Cells Analyzed: 600

Cells Analyzed: Interphase

Probes Utilized: BCR/ABL1

Thank you in advance,

Lilia.

[Guest guest]

Hi Zavie,

Thank you so much for your reply. I re-checked the copy. They didn't

specify the cytogenic result. I'll call to lab to clarify it. Do I

need to ask them: " How many positive cells were identified out of 20

cells tested? " Is this correct question to ask? Does it

mean " positive Ph chromosome " ?

I was on 400mlg of Gleevec for four weeks, then three weeks off

Gleevec, then about four weeks on 100mlg. Starting November 7th I'm

on 200mlg of Gleevec.

I'll email later today or tomorrow, as soon as I'll learn about the

cytogenic result.

Thank you,

Lilia.

>

> Hi Lilia,

>

> This is excellent news. It is moving in the right direction and at

a very

> good pace according to the FISH test..

>

> What I don't see is the cytogenic result. It is normally reported

as the

> number of cells that are +ive out of 20 cells tested.

>

> What dose of Gleevec are you on?

>

> Zavie

>

> Zavie (age 70)

> 67 Shoreham Avenue

> Ottawa, Canada, K2G 3X3

> dxd AUG/99

> INF OCT/99 to FEB/00, CHF

> No meds FEB/00 to JAN/01

> Gleevec since MAR/27/01 (400 mg)

> CCR SEP/01. #102 in Zero Club

> 2.8 log reduction Sep/05

> 3.0 log reduction Jan/06

> 2.9 log reduction Feb/07

> 3.6 log reduction Apr/08

> 3.6 log reduction Sep/08

> e-mail: zmiller@...

> Tel: 613-726-1117

> Fax: 309-296-0807

> Cell: 613-282-0204

> ID: zaviem

> YM: zaviemiller

> Skype: Zavie

>

> _____

>

> From: [mailto: ] On Behalf

Of

> llokl_777

> Sent: November 25, 2008 4:10 PM

>

> Subject: [ ] Need more help in understanding entire test results

>

> Hi Tracey,

>

> I got a copy of my bone marrow biopsy test results (in addition to

what

> I posted here last week). Unfortunately, they are not " bid news " ,

but I

> would be happy to understand what the results tell about the

condition

> of CML and if I'm moving into right direction and in appropriate

pace

> (I mean if the reduction that I have is good enough for three

months of

> treatment or it is kind of slow). This test was taken in three

months

> after the very first one. I'll really appreciate your feedback on

this

> one. Here is what I have written on the report:

>

> Clinical Indication: R/O CML

>

> Results: Female Karyotype and Abnormal FISH results positive for

cryptic

> BCR/ABL gene rearrangement

>

> Interpretation: No clonal cytogenetic abnormalities were detected in

> cells from short term unstimulated bone marrow aspirate

> cultures at the resolution obtained.

>

> FISH: Of a total of 600 interphase nuclei examined, 23.3% showed an

> abnormal positive single fusion signal. The current FISH results

> are consistent with continued involvement of bone marrow by the

> previously identified cell clone containing cryptic BCR/ABL gene

> rearrangement. Compared to the prior specimen (refer to 08/13/08,

> abnormal cells = 82.2%), this current specimen shows a decreased

> number of abnormal cells.

>

> Cytogenetic Analysis Summary:

> Number of Cells Analyzed: 20

> Number of Cultures used for Analysis: 2

> Number of Cells Karyotyped at Microscope: 14

> Number of Hard Copy Karyotypes: 6

> Banding Level: 350-450

> Banding Method: GTW/G

>

> FISH Analysis Summary:

> Number of Cells Analyzed: 600

> Cells Analyzed: Interphase

> Probes Utilized: BCR/ABL1

>

> Thank you in advance,

>

> Lilia.

>

>

>

>

[Guest guest]

Hi Lilia,

I agree with Zavie, these are very good FISH results for such a short

period of time. Let us know if you find out the cytogenetic results

as they don't seem to be here.

Also good news is that you don't appear to have any clonal evolutions

which can sometimes be trickier to treat and requires more frequent

monitoring for people who have them.

So far so good,

Tracey

> >

> > Hi Lilia,

> >

> > This is excellent news. It is moving in the right direction and

at

> a very

> > good pace according to the FISH test..

> >

> > What I don't see is the cytogenic result. It is normally reported

> as the

> > number of cells that are +ive out of 20 cells tested.

> >

> > What dose of Gleevec are you on?

> >

> > Zavie

> >

> > Zavie (age 70)

> > 67 Shoreham Avenue

> > Ottawa, Canada, K2G 3X3

> > dxd AUG/99

> > INF OCT/99 to FEB/00, CHF

> > No meds FEB/00 to JAN/01

> > Gleevec since MAR/27/01 (400 mg)

> > CCR SEP/01. #102 in Zero Club

> > 2.8 log reduction Sep/05

> > 3.0 log reduction Jan/06

> > 2.9 log reduction Feb/07

> > 3.6 log reduction Apr/08

> > 3.6 log reduction Sep/08

> > e-mail: zmiller@

> > Tel: 613-726-1117

> > Fax: 309-296-0807

> > Cell: 613-282-0204

> > ID: zaviem

> > YM: zaviemiller

> > Skype: Zavie

> >

> > _____

> >

> > From: [mailto: ] On

Behalf

> Of

> > llokl_777

> > Sent: November 25, 2008 4:10 PM

> >

> > Subject: [ ] Need more help in understanding entire test

results

> >

> > Hi Tracey,

> >

> > I got a copy of my bone marrow biopsy test results (in addition

to

> what

> > I posted here last week). Unfortunately, they are not " bid news " ,

> but I

> > would be happy to understand what the results tell about the

> condition

> > of CML and if I'm moving into right direction and in appropriate

> pace

> > (I mean if the reduction that I have is good enough for three

> months of

> > treatment or it is kind of slow). This test was taken in three

> months

> > after the very first one. I'll really appreciate your feedback on

> this

> > one. Here is what I have written on the report:

> >

> > Clinical Indication: R/O CML

> >

> > Results: Female Karyotype and Abnormal FISH results positive for

> cryptic

> > BCR/ABL gene rearrangement

> >

> > Interpretation: No clonal cytogenetic abnormalities were detected

in

> > cells from short term unstimulated bone marrow aspirate

> > cultures at the resolution obtained.

> >

> > FISH: Of a total of 600 interphase nuclei examined, 23.3% showed

an

> > abnormal positive single fusion signal. The current FISH results

> > are consistent with continued involvement of bone marrow by the

> > previously identified cell clone containing cryptic BCR/ABL gene

> > rearrangement. Compared to the prior specimen (refer to 08/13/08,

> > abnormal cells = 82.2%), this current specimen shows a decreased

> > number of abnormal cells.

> >

> > Cytogenetic Analysis Summary:

> > Number of Cells Analyzed: 20

> > Number of Cultures used for Analysis: 2

> > Number of Cells Karyotyped at Microscope: 14

> > Number of Hard Copy Karyotypes: 6

> > Banding Level: 350-450

> > Banding Method: GTW/G

> >

> > FISH Analysis Summary:

> > Number of Cells Analyzed: 600

> > Cells Analyzed: Interphase

> > Probes Utilized: BCR/ABL1

> >

> > Thank you in advance,

> >

> > Lilia.

> >

> >

> >

> >