amino acid disturbances in Chronic Fatigue Syndrome

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NMR metabolic profiling of serum identifies

amino acid disturbances in Chronic Fatigue Syndromehttp://bit.ly/O9izNJ

Clinica ChimicaActa

International Journal of Clinical Chemistry and

Diagnostic Laboratory Medicine

Available online 21 June 2012

In Press, Accepted Manuscript

Purchase $41.95:http://bit.ly/O9izNJ

NMR metabolicprofiling of serum identifies amino

acid disturbances in Chronic Fatigue Syndrome

W.Armstronga, b, Neil R.

McGregorc, R. Sheedyd, Ian Buttfielde,

Henry L. Buttb, f, R. Gooleya, b,

a Department ofBiochemistry and Molecular

Biology, University of Melbourne, Parkville,

3010, Australia

b Bio21 Molecular Science and Biotechnology

Institute, University of Melbourne, Parkville,

3010, Australia

c Faculty of Medicine, Dentistry & Health Sciences,

University of Melbourne, Parkville, 3010,

Australia

d Department of Zoology, The University of

Melbourne, Parkville, , 3010, Australia

e Child Development Centre, 90 Unley Road, Unley,

South Australia 5061, Australia

f Bioscreen, Building 404, Room G6, 2 Park Drive,

Parkville, 3010, Australia

Corresponding author at: 30 Flemington Road,

Parkville, VIC Australia 3010. Tel.: + 61 3 8344

2273; fax: + 61 3 9348 1421.

Received 27 April 2012. Revised 15 June 2012.

Accepted 15 June 2012. Available online 21 June 2012.

Abstract

Chronic fatiguesyndrome (CFS) is a debilitating multisystem disorder

characterized by long-term

fatigue with a variety of other symptoms including cognitive dysfunction,

unrefreshing sleep, muscle

pain, and post-exertional malaise.

It is a poorly understood condition that occurs in ~ 5 in every 1000

individuals.

We present here a preliminary study on the analysis of blood samples from 11 CFS

and 10

control subjects through NMR metabolic profiling.

Identified metabolites that were found to be significantly altered between the

groups were

subjected to correlation analysis to potentially elucidate disturbed metabolic

pathways.

Our results showed a significant reduction of glutamine (P = 0.002) and

ornithine (P & lt; 0.05) in

the blood of CFS samples.

Correlation analysis of glutamine and ornithine with other metabolites in the

CFS sera showed

relationships with glucogenic amino acids and metabolites that participate in

the urea cycle.

This indicates a possible disturbance to amino acid and nitrogen metabolism. It

would be beneficial to

identify any potential biomarkers of CFS for accurate diagnosis of the disorder.

Copyright © 2012 Published by Elsevier B.V.

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